产品名称 |
OE19人食管癌细胞 |
货号 |
ZQ0552 |
产品介绍 |
细胞系 OE19,也称为 JROECL19,于 1993 年从一名 72 岁男性患者的贲门/食管胃交界处的腺癌中建立。肿瘤被确定为病理分期 III (UICC) 并显示出中度分化。细胞系 OE19 组成型表达 HLA-A、-B 和 -C 抗原(MHC I 类)。用干扰素-γ 处理诱导 ICAM-1 (CD54) 的表达。仅在 OE19 亚群中测量了添加干扰素-γ 时 HLA-DR(MHC II 类)的表达。这些细胞表达上皮细胞角蛋白,并且在裸鼠中具有致瘤性。在 ECACC 测试时,通过短串联重复序列 (STR)-PCR 分析无法在该细胞系中检测到 Y 染色体。众所周知,由于癌细胞系的遗传不稳定性增加,Y 染色体可能会重新排列或丢失,从而导致检测不到。根据 STR-PCR 分析,细胞系与保藏者提供的来源相同。 |
种属 |
人 |
性别/年龄 |
72岁/男性 |
组织 |
食道/胃贲门 |
疾病 |
食管癌 |
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮样 |
生长方式 |
贴壁生长为单层 |
倍增时间 |
~50-60 hours (DSMZ=ACC-700) |
培养基和添加剂 |
90%RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%双抗(品牌:中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
基因表达 |
*** |
保藏机构 |
DSMZ; ACC-700ECACC; 96071721 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0552 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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