产品名称 |
NCI-H146人小细胞肺癌细胞 |
货号 |
ZQ0723 |
产品介绍 |
NCI-H146是一种人小细胞肺癌细胞系,最初由A.F. Gazdar等人于1979年从一位59岁男性小细胞肺癌患者骨髓转移灶中建立。细胞呈淋巴母细胞样,悬浮生长,易形成细胞聚团。具有近三倍体特性,主要染色体数为68,部分细胞染色体数目在66至71之间波动。细胞表达SCLC特有的4种生化标记物水平升高:神经元特异性烯醇化酶、肌酸激酶脑同功酶、左旋多巴脱羧酶和蛙皮素样免疫反应性;细胞角蛋白和波形蛋白染色阳性,而神经丝三联体蛋白染色阴性;细胞表达相对较高数量的c-myc mRNA,但未检测到该基因的扩增。 【注意事项】该细胞传代时可以通过加入新鲜培养基稀释或者通过离心收集并将细胞重悬浮在新鲜培养基中。 |
种属 |
人 |
性别/年龄 |
男/59岁 |
组织 |
肺 |
疾病 |
癌 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮样 |
生长方式 |
悬浮 |
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%胎牛血清10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
STR位点信息 |
Amelogenin: X CSF1PO: 11 D13S317: 11,12 D16S539: 11 D5S818: 12 D7S820: 9,10 THO1: 6,9.3 TPOX: 8,11 vWA: 14,16 |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; HTB-173;BCRC; 60280 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0723 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties.
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Monoclonal antibodies that demonstrate specificity for several types of human lung cancer.
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MicroRNA expression distinguishes SCLC from NSCLC lung tumor cells and suggests a possible pathological relationship between SCLCs and NSCLCs.
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Proteomic profiling identifies dysregulated pathways in small cell lung cancer and novel therapeutic targets including PARP1.
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