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BxPC-3人原位胰腺腺癌细胞(STR鉴定)
英文名:BxPC-3
货号:ZQ0064
价格:¥1400.00
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BxPC-3人原位胰腺腺癌细胞(STR鉴定)

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BxPC-3人原位胰腺腺癌细胞专用培养基

¥350.00 ¥450.00

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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

BxPC-3人原位胰腺腺癌细胞

货号

ZQ0064

产品介绍

BxPC-3是一种人原位胰腺腺癌细胞系,于1980年从一名罹患胰腺癌的61岁女性的原位胰腺癌组织切片中分离建系,呈上皮细胞形态。该细胞是一种合适的转染宿主,可用于三维培养和癌症研究。该细胞表达粘蛋白(mucin)、胰腺癌相关抗原和癌胚抗原(CEA),但不表达囊性纤维化跨膜传导调节因子(CFTR)。据报道,该细胞可在裸鼠体内成瘤。BxPC-3细胞系是研究胰腺癌的重要工具,广泛用于胰腺癌的研究,尤其是在探讨肿瘤形成和治疗策略方面,提供了一个模型来研究癌症的生物学特性和测试新的治疗方法。

注意事项:
(1)BxPC-3细胞在高密度传代时细胞生长较快,低密度时细胞生长较慢。
(2)细胞传代后细胞培养需要24-48小时贴壁,48小时后再换液操作。

种属

性别/年龄

女/61岁

组织

胰腺

疾病

腺癌

细胞类型

肿瘤细胞 

形态学

上皮

生长方式

贴壁

倍增时间

大约48~60小时 

培养基和添加剂

RPMI-1640基础培养基(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500+1%P/S(中乔新舟 货号:CSP006

推荐完全培养基货号

ZM0064 

生物安全等级

BSL-1

STR位点信息

Amelogenin: X
D3S1358: 14,16
TH01: 9
D21S11: 29
D18S51: 12
Penta_E: 12,14
D5S818: 11
D13S317: 11
D7S820: 10,13
D16S539: 9,11
CSF1PO: 13
Penta_D: 14
vWA: 14,18
D8S1179: 13
TPOX: 8
FGA: 20,21
D19S433: 13,16.2
D2S1338: 17,19

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

*** 

基因表达

*** 

保藏机构

ATCC; CRL-1687 BCRC; 60283 BCRJ; 0056 DSMZ; ACC-760 ECACC; 93120816

供应限制

仅供科研使用

货号

ZQ0064

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


1、论文标题: Ångstrom‐Scale Silver Particles as a Promising Agent for Low‐Toxicity Broad‐Spectrum Potent Anticancer Therapy
DOI: 10.1002/adfm.201808556
发表时间: 2019-02-27
期刊: ADVANCED FUNCTIONAL MATERIALS
影响因子: 15.621
货号: ZQ0064
产品名称: BxPC-3 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/adfm.201808556


2、论文标题: The Role of Long Noncoding RNA AL161431.1 in the Development and Progression of Pancreatic Cancer
DOI: 10.3389/fonc.2021.666313
发表时间: 2021-07-30
期刊: Frontiers in Oncology
影响因子: 6.244
货号: ZQ0064
产品名称: BxPC-3 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8363261/


3、论文标题: NEAT1/miR-101-dependent Up-regulation of DNA-PKcs Enhances Malignant Behaviors of Pancreatic Ductal Adenocarcinoma Cells
DOI: 10.7150/jca.58824
发表时间: 2021-07-25
期刊: Journal of Cancer
影响因子: 4.207
货号: ZQ0064
产品名称: BxPC-3 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8364653/


4、论文标题: The biological behavior of tRNA-derived fragment tRF-Leu-AAG in pancreatic cancer cells
DOI: 10.1080/21655979.2022.2064206
发表时间: 2022-04-20
期刊: Bioengineered
影响因子: 3.269
货号: ZQ0064
产品名称: BxPC-3 cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/21655979.2022.2064206


5、论文标题: Osteoglycin (OGN) promotes tumorigenesis of pancreatic cancer cell via targeting ID4
DOI: 10.1016/j.tice.2022.101867
发表时间: 2022-07-16
期刊: TISSUE & CELL
影响因子: 2.586
货号: ZQ0064
产品名称: BxPC-3 cell line

原文链接: https://www.sciencedirect.com/science/article/pii/S0040816622001392


6、PubMed=27229158; DOI=10.1038/cgt.2016.23

Schreiber R., Mezencev R., Matyunina L.V., McDonald J.F.
Evidence for the role of microRNA 374b in acquired cisplatin resistance in pancreatic cancer cells.
Cancer Gene Ther. 23:241-245(2016)


7、PubMed=27259358; DOI=10.1074/mcp.M116.058313
Humphrey E.S., Su S.-P., Nagrial A.M., Hochgrafe F., Pajic M., Lehrbach G.M., Parton R.G., Yap A.S., Horvath L.G., Chang D.K., Biankin A.V., Wu J.-M., Daly R.J.
Resolution of novel pancreatic ductal adenocarcinoma subtypes by global phosphotyrosine profiling.
Mol. Cell. Proteomics 15:2671-2685(2016)


8、PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)


9、PubMed=27910856; DOI=10.1038/cgt.2016.71
Mezencev R., Matyunina L.V., Wagner G.T., McDonald J.F.
Acquired resistance of pancreatic cancer cells to cisplatin is multifactorial with cell context-dependent involvement of resistance genes.
Cancer Gene Ther. 23:446-453(2016)


10、PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)


11、PubMed=29444439; DOI=10.1016/j.celrep.2018.01.051
Yuan T.L., Amzallag A., Bagni R., Yi M., Afghani S., Burgan W., Fer N., Strathern L.A., Powell K., Smith B., Waters A.M., Drubin D.A., Thomson T., Liao R., Greninger P., Stein G.T., Murchie E., Cortez E., Egan R.K., Procter L., Bess M., Cheng K.T., Lee C.-S., Lee L.C., Fellmann C., Stephens R., Luo J., Lowe S.W., Benes C.H., McCormick F.
Differential effector engagement by oncogenic KRAS.
Cell Rep. 22:1889-1902(2018)


12、PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)


13、PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)


14、PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)


15、PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)


16、PubMed=32782605; DOI=10.3892/ol.2020.11825
Takagi K., Imura J., Shimomura A., Noguchi A., Minamisaka T., Tanaka S., Nishida T., Hatta H., Nakajima T.
Establishment of highly invasive pancreatic cancer cell lines and the expression of IL-32.
Oncol. Lett. 20:2888-2896(2020)


17、PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)

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