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RAMOS 人B淋巴细胞瘤细胞 (STR鉴定)
英文名:RAMOS human B lymphoblastoma cells
货号:ZQ1021
价格:¥1500.00
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推荐组合

RAMOS 人B淋巴细胞瘤细胞 (STR鉴定)

¥1500.00
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RAMOS人B淋巴细胞瘤细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

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细胞套餐惊爆价

¥1850 ¥1980.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

RAMOS 人B淋巴细胞瘤细胞

货号

ZQ1021

产品介绍

Ramos (RA 1) 是一种 B 淋巴细胞系,来源于一名患有伯基特 Burkitt 淋巴瘤的 3 岁男孩的腹水中建立的,是免疫学研究的重要资源。该细胞系以分泌 IgM 为特征,对于 B 细胞表面抗原分析、细胞毒性药物检测、突变分析和凋亡机制探索具有重要价值。


RAMOS 细胞表现出淋巴母细胞样形态,并以其体外强劲生长而闻名。它们在与 B 细胞发育、功能和恶性肿瘤相关的研究中特别有价值,包括研究 B 细胞受体 (BCR) 信号通路、基因表达以及正常 B 细胞转化为恶性细胞的潜在机制。

由于这些细胞具有 B 细胞谱系,因此它们也经常用于抗体生产研究,使研究人员能够探索 B 细胞对各种抗原的反应以及随后的抗体生成。RAMOS 细胞还用于药物发现和毒性研究。它们对各种化疗药物的敏感性使它们成为新癌症疗法临床前评估的宝贵工具。

值得注意的是,拉莫斯细胞系是 EBV 阴性的,为研究不受 Epstein-Barr 病毒影响的伯基特淋巴瘤提供了基线模型。是 B 细胞生物学和伯基特淋巴瘤研究中的宝贵资产,在探索 B 细胞发育、恶性肿瘤、抗体产生和新癌症疗法的疗效方面发挥着重要作用。

特别注意:该细胞为悬浮细胞,请注意离心收集细胞悬液。细胞形态有部分悬浮聚小团细胞是正常现象

种属

白人

性别/年龄

/3岁

组织

淋巴

疾病

伯基茨淋巴瘤

细胞类型

肿瘤细胞

形态学

圆形

生长方式

悬浮

倍增时间

20.4 +- 1.5 hours (PubMed=9225077); 23 hours (PubMed=8402660); 14 hours (PubMed=20922763); ~48 hours (DSMZ=ACC-603)

培养基和添加剂

RPMI-1640(品牌:中乔新舟 货号:ZQ-200+10%胎牛血清(中乔新舟  货号:ZQ500-A+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM1021

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37℃

STR位点信息

Amelogenin: X

CSF1PO 10,11

D1S1656 12,15.3

D2S441 11,14

D2S1338 20,23

D3S1358 14,15

D5S818 7,12

D6S1043 13,15

D7S820 11

D8S1179 13 (CLS=302007)

              :13,16 (ATCC=CRL-1596; CCRID; DepMap=ACH-001636;                                                DSMZ=ACC-603; Technion Genomics Center BCF;                                                PubMed=20922763; PubMed=25877200)

D10S1248 14,15

D12S391 19,22

D13S317 12,13,14 (CLS=302007)

              :13,14 (ATCC=CRL-1596; CCRID; COG; DepMap=ACH-001636;                                        DSMZ=ACC-603; ECACC=85030802; Technion Genomics                                    Center                                                                                                                    BCF; JCRB=JCRB9119; KCLB=21596; PubMed=20922763;                                    PubMed=25877200)

D16S539 10,13

D18S51 14,15 (CLS=302007; DSMZ=ACC-603)

             :15 (ATCC=CRL-1596; CCRID; DepMap=ACH-001636; Technion                                  Genomics Center BCF; PubMed=20922763; PubMed=25877200)

D19S433 14,15.2

D21S11 30

D22S1045 15

FGA 20,24

Penta D 10,13

Penta E 6,8,21 (CLS=302007)

            :8,21 (ATCC=CRL-1596; CCRID; DepMap=ACH-001636; DSMZ=ACC-                    603; Technion Genomics Center BCF; PubMed=25877200)

TH01 7,9.3

TPOX 8,9

vWA 15,16

抗原表达/受体表达

*** 

基因表达

*** 

保藏机构

ATCC; CRL-1596

供应限制

仅供科研使用


货号

ZQ1021

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

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An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics. EBV infectibility and permanent conversion into EBV-positive sublines by in vitro infection.
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Inducibility of the Epstein-Barr virus (EBV) cycle and surface marker properties of EBV-negative lymphoma lines and their in vitro EBV-converted sublines.
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PubMed=175026; DOI=10.1002/ijc.2910170203
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Establishment of EBNA-expressing cell lines by infection of Epstein-Barr virus (EBV)-genome-negative human lymphoma cells with different EBV strains.
Int. J. Cancer 17:161-166(1976)


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Povey S., Jeremiah S., Arthur E., Steel M., Klein G.
Differences in genetic stability between human cell lines from patients with and without lymphoreticular malignancy.
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PubMed=6286763; DOI=10.4049/jimmunol.129.3.1336
Benjamin D., Magrath I.T., Maguire R.T., Janus C., Todd H.D., Parsons R.G.
Immunoglobulin secretion by cell lines derived from African and American undifferentiated lymphomas of Burkitt's and non-Burkitt's type.
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PubMed=6231253; DOI=10.1002/ijc.2910330407
Ehlin-Henriksson B., Klein G.
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Int. J. Cancer 33:459-463(1984)


PubMed=2985879; DOI=10.1016/0145-2126(85)90084-0
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cell lines -- 1 carboxylic esterase.
Leuk. Res. 9:209-229(1985)


PubMed=2998993
Steel C.M., Morten J.E.N., Foster E.
The cytogenetics of human B lymphoid malignancy: studies in Burkitt's lymphoma and Epstein-Barr virus-transformed lymphoblastoid cell lines.
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PubMed=3159941; DOI=10.1016/0145-2126(85)90134-1
Drexler H.G., Gaedicke G., Minowada J.
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Leuk. Res. 9:549-559(1985)


PubMed=3874327; DOI=10.1016/0145-2126(85)90133-x
Drexler H.G., Gaedicke G., Minowada J.
Isoenzyme studies in human leukemia-lymphoma cells lines -- II. Acid phosphatase.
Leuk. Res. 9:537-548(1985)


PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
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PubMed=2835030; DOI=10.1016/S0385-8146(87)80025-1
Takimoto T., Sato H., Ogura H., Miyazaki T.
Establishment of an Epstein-Barr virus (EBV) genome-positive subline of Ramos (Ramos/NPC) following infection of Ramos with nasopharyngeal carcinoma (NPC)-derived EBV.
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PubMed=3026973; DOI=10.1002/ijc.2910390215
Ehlin-Henriksson B., Manneborg-Sandlund A., Klein G.
Expression of B-cell-specific markers in different Burkitt lymphoma subgroups.
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PubMed=1850347; DOI=10.1210/endo-128-5-2266
Baglia L.A., Cruz D., Shaw J.E.
An Epstein-Barr virus-negative Burkitt lymphoma cell line (sfRamos) secretes a prolactin-like protein during continuous growth in serum-free medium.
Endocrinology 128:2266-2272(1991)


PubMed=1915267; DOI=10.1002/j.1460-2075.1991.tb07837.x
Farrell P.J., Allan G.J., Shanahan F., Vousden K.H., Crook T.
p53 is frequently mutated in Burkitt's lymphoma cell lines.
EMBO J. 10:2879-2887(1991)


PubMed=2052620; DOI=10.1073/pnas.88.12.5413
Gaidano G., Ballerini P., Gong J.Z., Inghirami G., Neri A., Newcomb E.W., Magrath I.T., Knowles D.M., Dalla-Favera R.
p53 mutations in human lymphoid malignancies: association with Burkitt lymphoma and chronic lymphocytic leukemia.
Proc. Natl. Acad. Sci. U.S.A. 88:5413-5417(1991)


PubMed=8316623; DOI=10.2307/3578190
Evans H.H., Ricanati M., Horng M.-F., Jiang Q.-Y., Mencl J., Olive P.L.
DNA double-strand break rejoining deficiency in TK6 and other human B-lymphoblast cell lines.
Radiat. Res. 134:307-315(1993)


PubMed=8344493; DOI=10.1096/fasebj.7.10.8344493
Bhatia K.G., Goldschmidts W., Gutierrez M.I., Gaidano G., Dalla-Favera R., Magrath I.T.
Hemi- or homozygosity: a requirement for some but not other p53 mutant proteins to accumulate and exert a pathogenetic effect.
FASEB J. 7:951-956(1993)


PubMed=8402660
O'Connor P.M., Jackman J., Jondle D., Bhatia K.G., Magrath I.T., Kohn K.W.
Role of the p53 tumor suppressor gene in cell cycle arrest and radiosensitivity of Burkitt's lymphoma cell lines.
Cancer Res. 53:4776-4780(1993)


PubMed=8515068; DOI=10.4049/jimmunol.150.12.5418
Jain V.K., Judde J.-G., Max E.E., Magrath I.T.
Variable IgH chain enhancer activity in Burkitt's lymphomas suggests an additional, direct mechanism of c-myc deregulation.
J. Immunol. 150:5418-5428(1993)


PubMed=7757991
Bae I., Fan S.-J., Bhatia K.G., Kohn K.W., Fornace A.J. Jr., O'Connor P.M.
Relationships between G1 arrest and stability of the p53 and p21Cip1/Waf1 proteins following gamma-irradiation of human lymphoma cells.
Cancer Res. 55:2387-2393(1995)


PubMed=8896424; DOI=10.1182/blood.V88.9.3562.bloodjournal8893562
Chapman C.J., Zhou J.X., Gregory C.D., Rickinson A.B., Stevenson F.K.
VH and VL gene analysis in sporadic Burkitt's lymphoma shows somatic hypermutation, intraclonal heterogeneity, and a role for antigen selection.
Blood 88:3562-3568(1996)


PubMed=9192833
Cherney B.W., Bhatia K.G., Sgadari C., Gutierrez M.I., Mostowski H.S., Pike S.E., Gupta G., Magrath I.T., Tosato G.
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Cancer Res. 57:2508-2515(1997)


PubMed=9225077; DOI=10.1016/S0145-2126(97)00126-4
Okano M.
High susceptibility of an Epstein-Barr virus-converted Burkitt's lymphoma cell line to cytotoxic drugs.
Leuk. Res. 21:469-471(1997)


PubMed=9473234; DOI=10.1182/blood.V91.5.1680
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p16/INK4a and p15/INK4b gene methylation and absence of p16/INK4a mRNA and protein expression in Burkitt's lymphoma.
Blood 91:1680-1687(1998)


PubMed=9973220
Gutierrez M.I., Cherney B.W., Hussain A., Mostowski H.S., Tosato G., Magrath I.T., Bhatia K.G.
Bax is frequently compromised in Burkitt's lymphomas with irreversible resistance to Fas-induced apoptosis.
Cancer Res. 59:696-703(1999)


PubMed=10739008; DOI=10.1016/S0145-2126(99)00182-4
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The c-MYC allele that is translocated into the IgH locus undergoes constitutive hypermutation in a Burkitt's lymphoma line.
Oncogene 19:3404-3410(2000)


PubMed=11226526; DOI=10.1016/S0145-2126(00)00121-1
Inoue K., Kohno T., Takakura S., Hayashi Y., Mizoguchi H., Yokota J.
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PubMed=12967475; DOI=10.1111/j.1349-7006.2003.tb01518.x
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PubMed=18211290; DOI=10.1111/j.1365-2184.2007.00500.x
Zander Balderud L., Bemark M.
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Cell Prolif. 41:136-155(2008)


PubMed=20922763; DOI=10.1002/pbc.22801
Kang M.H., Smith M.A., Morton C.L., Keshelava N., Houghton P.J., Reynolds C.P.
National Cancer Institute pediatric preclinical testing program: model description for in vitro cytotoxicity testing.
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PubMed=22885699; DOI=10.1038/nature11378
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PubMed=24590883; DOI=10.1002/gcc.22161
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PubMed=25960936; DOI=10.4161/21624011.2014.954893
Boegel S., Lower M., Bukur T., Sahin U., Castle J.C.
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PubMed=25485619; DOI=10.1038/nbt.3080
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PubMed=25877200; DOI=10.1038/nature14397
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PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
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