名称 |
LLC-MK2恒河猴肾细胞(种属鉴定) |
货号 |
ZQ0162 |
产品介绍 |
LLC-MK2细胞株源自六只成年绿猴的肾细胞悬液。 据报道这个细胞对很多病毒敏感,包括肠道病毒、鼻病毒、粘病毒和痘病毒家族中的成员。 |
种属 |
恒河猴 |
组织来源 |
原位;肾 |
形态学 |
上皮样 |
细胞类型 |
自发永生化细胞系 |
倍增时间 |
|
生长方式 |
贴壁 |
培养基和添加剂 |
DMEM高糖(品牌:中乔新舟 货号:ZQ-100)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
AddexBio; P0014004/4952 ATCC; CCL-7 ATCC; CCL-7.1 BCRC; 60092 BCRC; 60365 BCRJ; 0146 CCTCC; GDC0156 CLS; 305149 ECACC; 85062804 IZSLER; BS CL 57 |
供应限制 |
仅供科研使用 |
货号 |
ZM0162 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=13821019; DOI=10.1093/oxfordjournals.aje.a120078
Evans V.J., Kerr H.A., McQuilkin W.T., Earle W.R., Hull R.N.
Growth in vitro of a long-term strain of monkey-kidney cells in medium NCTC 109 free of any added protein.
Am. J. Hyg. 70:297-302(1959)
DOI=10.1002/jbmte.390020304
Cheery W.R., Hull R.N.
Studies and observations on the growth of mammalian cells in agitated fluid medium.
J. Biochem. Microbiol. Technol. Eng. 2:267-285(1960)
PubMed=14473108; DOI=10.4049/jimmunol.88.6.796
Meyer H.M. Jr., Hopps H.E., Rogers N.G., Brooks B.E., Bernheim B.C., Jones W.P., Nisalak A., Douglas R.D.
Studies on simian virus 40.
J. Immunol. 88:796-806(1962)
PubMed=14449901; DOI=10.1084/jem.115.5.903
Hull R.N., Cherry W.R., Tritch O.J.
Growth characteristics of monkey kidney cell strains LLC-MK1, LLC-MK2, and LLC-MK2(NCTC-3196) and their utility in virus research.
J. Exp. Med. 115:903-918(1962)
PubMed=14449902
Hull R.N., Tritch O.J.
Characterization of cell strains by viral susceptibility.
Natl. Cancer Inst. Monogr. 7:161-172(1962)
PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)
DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)
PubMed=18605421; DOI=10.1093/jnci/45.5.951
Ikeuchi T., Sandberg A.A.
Chromosome pulverization in virus-induced heterokaryons of mammalian cells from different species.
J. Natl. Cancer Inst. 45:951-963(1970)
PubMed=4203458
Petricciani J.C., Wallace R.E., McCoy D.W.
A comparison of three in vivo assays for cell tumorigenicity.
Cancer Res. 34:105-108(1974)
Patent=US3904480
Hull R.N., Huseby R.M.
Enhanced production of plasminogen activator.
Patent number US3904480, 09-Sep-1975
PubMed=6298990; DOI=10.1016/0378-1135(82)90056-6
McPhee D.A., Parsonson I.M., Della-Porta A.J.
Comparative studies on the growth of Australian bluetongue virus serotypes in continuous cell lines and embryonated chicken eggs.
Vet. Microbiol. 7:401-410(1982)
PubMed=16494729; DOI=10.3201/eid1201.050496
Kaye M., Druce J., Tran T., Kostecki R., Chibo D., Morris J., Catton M., Birch C.
SARS-associated coronavirus replication in cell lines.
Emerg. Infect. Dis. 12:128-133(2006)
PubMed=28046048; DOI=10.1371/journal.pone.0169391
Dotti S., Lombardo T., Villa R., Cacciamali A., Zanotti C., Andreani N.A., Cinotti S., Ferrari M.
Transformation and tumorigenicity testing of simian cell lines and evaluation of poliovirus replication.
PLoS ONE 12:E0169391-E0169391(2017)
PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)
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