名称 |
DH82狗肾恶性组织细胞增生症细胞(种属鉴定) |
货号 |
ZQ0168 |
产品介绍 |
DH82细胞具有类似巨噬细胞形态,并能吞噬乳胶颗粒。细胞具有Fc gamma受体阳性,Fc mu和C3b受体阴性。细胞不生成IL-1。 |
种属 |
狗 |
组织来源 |
原位;肾 |
形态学 |
巨噬细胞样 |
细胞类型 |
肿瘤细胞 |
倍增时间 |
26 hours (PubMed=27257868) |
生长方式 |
贴壁 |
培养基和添加剂 |
MEM(中乔新舟 货号:ZQ-300)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
ATCC; CRL-3590 BCRC; 60110 BCRJ; 0077 CLS; 305003 ECACC; 94062922 ICLC; ATL96008 KCB; KCB 2014070YJ |
供应限制 |
仅供科研使用 |
货号 |
ZM0168 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=3350786; DOI=10.1007/BF02623551
Wellman M.L., Krakowka S., Jacobs R.M., Kociba G.J.
A macrophage-monocyte cell line from a dog with malignant histiocytosis.
In Vitro Cell. Dev. Biol. 24:223-229(1988)
Patent=US5192679
Dawson J.E., Rikihisa Y.
Growing Ehrlichia species in a continuous cell line.
Patent number US5192679, 09-Mar-1993
PubMed=11377712; DOI=10.1016/S0166-0934(01)00288-9
Hines R., Maury W.
DH82 cells: a macrophage cell line for the replication and study of equine infectious anemia virus.
J. Virol. Methods 95:47-56(2001)
PubMed=11585095
Zweygarth E., Josemans A.I.
Continuous in vitro propagation of Cowdria ruminantium (Welgevonden stock) in a canine macrophage-monocyte cell line.
Onderstepoort J. Vet. Res. 68:155-157(2001)
PubMed=12234001
Zweygarth E., Josemans A.I., Van Strijp M.F., Van Heerden H., Allsopp M.T.E.P., Allsopp B.A.
The Kumm isolate of Ehrlichia ruminantium: in vitro isolation, propagation and characterization.
Onderstepoort J. Vet. Res. 69:147-153(2002)
PubMed=21908323; DOI=10.1177/1040638711408064
O'Donoghue L.E., Rivest J.P., Duval D.L.
Polymerase chain reaction-based species verification and microsatellite analysis for canine cell line validation.
J. Vet. Diagn. Invest. 23:780-785(2011)
PubMed=25534080; DOI=10.1016/j.vetimm.2014.11.006
Heinrich F., Contioso V.B., Stein V.M., Carlson R., Tipold A., Ulrich R., Puff C., Baumgartner W., Spitzbarth I.
Passage-dependent morphological and phenotypical changes of a canine histiocytic sarcoma cell line (DH82 cells).
Vet. Immunol. Immunopathol. 163:86-92(2015)
PubMed=27257868; DOI=10.1371/journal.pone.0156689
Maeda J., Froning C.E., Brents C.A., Rose B.J., Thamm D.H., Kato T.A.
Intrinsic radiosensitivity and cellular characterization of 27 canine cancer cell lines.
PLoS ONE 11:E0156689-E0156689(2016)
PubMed=26842912; DOI=10.1111/vco.12197
Hartley G., Faulhaber E.A., Caldwell A., Coy J., Kurihara J.N., Guth A., Regan D.P., Dow S.W.
Immune regulation of canine tumour and macrophage PD-L1 expression.
Vet. Comp. Oncol. 15:534-549(2017)
PubMed=27197945; DOI=10.1111/vco.12192
Fowles J.S., Dailey D.D., Gustafson D.L., Thamm D.H., Duval D.L.
The Flint Animal Cancer Center (FACC) canine tumour cell line panel: a resource for veterinary drug discovery, comparative oncology and translational medicine.
Vet. Comp. Oncol. 15:481-492(2017)
PubMed=28620374; DOI=10.3389/fimmu.2017.00604
Mendonca P.H.B., da Rocha R.F.D.B., de Braz Moraes J.B., LaRocque-de-Freitas I.F., Logullo J., Morrot A., Nunes M.P., Freire-de-Lima C.G., Decote-Ricardo D.
Canine macrophage DH82 cell line as a model to study susceptibility to Trypanosoma cruzi infection.
Front. Immunol. 8:604.1-604.11(2017)
PubMed=31175136; DOI=10.1158/1535-7163.MCT-18-1346
Das S., Idate R., Cronise K.E., Gustafson D.L., Duval D.L.
Identifying candidate druggable targets in canine cancer cell lines using whole exome sequencing.
Mol. Cancer Ther. 18:1460-1471(2019)
PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)
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