名称 |
IPEC-J2 猪肠上皮细胞(种属鉴定) |
货号 |
ZQ1078 |
产品介绍 |
由从新生未哺乳猪的空肠中分离出的正常肠上皮细胞建立。 |
种属 |
猪 |
组织来源 |
原位;小肠,空肠 |
形态学 |
上皮样 |
细胞类型 |
自发永生化细胞 |
倍增时间 |
~40-60 hours (DSMZ=ACC-701) |
生长方式 |
贴壁 |
培养基和添加剂 |
|
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
|
供应限制 |
仅供科研使用 |
货号 |
ZQ1078 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
DOI=10.1016/S0016-5085(89)80002-2
Berschneider H.M.
Development of normal cultured small intestinal epithelial cell lines which transport Na and Cl.
Gastroenterology 96:A41-A41(1989)
PubMed=16215741; DOI=10.1007/s00418-005-0067-z
Schierack P., Nordhoff M., Pollmann M., Weyrauch K.D., Amasheh S., Lodemann U., Jores J., Tachu B., Kleta S., Blikslager A., Tedin K., Wieler L.H.
Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine.
Histochem. Cell Biol. 125:293-305(2006)
PubMed=21626283; DOI=10.1007/s10616-011-9362-9
Geens M.M., Niewold T.A.
Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization.
Cytotechnology 63:415-423(2011)
PubMed=22074860; DOI=10.1016/j.vetmic.2011.10.017
Brosnahan A.J., Brown D.R.
Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations.
Vet. Microbiol. 156:229-237(2012)
PubMed=22193509; DOI=10.1007/s10616-011-9420-3
Steube K.G., Koelz A.-L., Uphoff C.C., Drexler H.G., Kluess J., Steinberg P.
The necessity of identity assessment of animal intestinal cell lines: a case report.
Cytotechnology 64:373-378(2012)
DOI=10.1155/2013/980651
Stoy A.C.F., Heegaard P.M.H., Sangild P.T., Ostergaard M.V., Skovgaard K.
Gene expression analysis of the IPEC-J2 cell line: a simple model for the inflammation-sensitive preterm intestine.
ISRN Genomics 2013:980651.1-980651.7(2013)
PubMed=24260272; DOI=10.1371/journal.pone.0079643
Zakrzewski S.S., Richter J.F., Krug S.M., Jebautzke B., Lee I.-F.M., Rieger J., Sachtleben M., Bondzio A., Schulzke J.-D., Fromm M., Gunzel D.
Improved cell line IPEC-J2, characterized as a model for porcine jejunal epithelium.
PLoS ONE 8:E79643-E79643(2013)
PubMed=25407967; DOI=10.1186/s12985-014-0193-0
Yan M.-F., Zhu L.-Q., Yang Q.
Infection of porcine circovirus 2 (PCV2) in intestinal porcine epithelial cell line (IPEC-J2) and interaction between PCV2 and IPEC-J2 microfilaments.
Virol. J. 11:193.1-193.9(2014)
PubMed=26147118; DOI=10.1371/journal.pone.0132323
Nossol C., Barta-Boszormenyi A., Kahlert S., Zuschratter W., Faber-Zuschratter H., Reinhardt N., Ponsuksili S., Wimmers K., Diesing A.-K., Rothkotter H.-J.
Comparing two intestinal porcine epithelial cell lines (IPECs): morphological differentiation, function and metabolism.
PLoS ONE 10:E0132323-E0132323(2015)
PubMed=29787056; DOI=10.1007/978-3-319-16104-4_12
Vergauwen H.
The IPEC-J2 cell line.
(In) The impact of food bioactives on health. In vitro and ex vivo models; Verhoeckx K., Cotter P., Lopez-Exposito I., Kleiveland C., Lea T., Mackie A., Requena T., Swiatecka D., Wichers H. (eds.); pp.125-134; Springer; Cham (2015)
PubMed=26651362; DOI=10.1021/acs.molpharmaceut.5b00874
Saaby L., Helms H.C.C., Brodin B.
IPEC-J2 MDR1, a novel high-resistance cell line with functional expression of human P-glycoprotein (ABCB1) for drug screening studies.
Mol. Pharm. 13:640-652(2016)
PubMed=26975772; DOI=10.4014/jmb.1512.12075
Kim S.H., Pajarillo E.A.B., Balolong M.P., Lee J.Y., Kang D.-K.
Constructing proteome reference map of the porcine jejunal cell line (IPEC-J2) by label-free mass spectrometry.
J. Microbiol. Biotechnol. 26:1124-1131(2016)
PubMed=32708885; DOI=10.3390/pharmaceutics12070673
Saaby L., Trasborg J., Rasmussen M.A., Holst B., Brodin B.
IPEC-J2 rMdr1a, a new cell line with functional expression of rat P-glycoprotein encoded by rat Mdr1a for drug screening purposes.
Pharmaceutics 12:673.1-673.17(2020)