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IPEC-J2 猪肠上皮细胞
英文名:IPEC-J2
货号:ZQ1078
价格:¥3800.00
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IPEC-J2 猪肠上皮细胞

¥3800.00
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IPEC-J2猪肠上皮细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

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细胞套餐惊爆价

¥4150 ¥4280.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

名称

IPEC-J2 猪肠上皮细胞(种属鉴定)

货号

ZQ1078

产品介绍

由从新生未哺乳猪的空肠中分离出的正常肠上皮细胞建立。

种属

组织来源

原位;小肠,空肠

形态学

上皮样

细胞类型

自发永生化细胞

倍增时间

~40-60 hours (DSMZ=ACC-701)

生长方式

贴壁

培养基和添加剂

DMEM (中乔新舟  货号:ZQ-100)+10%胎牛血清(中乔新舟  货号:ZQ500-A

推荐完全培养基货号

ZM1078

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37℃

保藏机构

DSMZ; ACC-701
IZSLER; BS CL 205

供应限制

仅供科研使用


货号

ZQ1078

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

DOI=10.1016/S0016-5085(89)80002-2
Berschneider H.M.
Development of normal cultured small intestinal epithelial cell lines which transport Na and Cl.
Gastroenterology 96:A41-A41(1989)

PubMed=16215741; DOI=10.1007/s00418-005-0067-z
Schierack P., Nordhoff M., Pollmann M., Weyrauch K.D., Amasheh S., Lodemann U., Jores J., Tachu B., Kleta S., Blikslager A., Tedin K., Wieler L.H.
Characterization of a porcine intestinal epithelial cell line for in vitro studies of microbial pathogenesis in swine.
Histochem. Cell Biol. 125:293-305(2006)

PubMed=21626283; DOI=10.1007/s10616-011-9362-9
Geens M.M., Niewold T.A.
Optimizing culture conditions of a porcine epithelial cell line IPEC-J2 through a histological and physiological characterization.
Cytotechnology 63:415-423(2011)

PubMed=22074860; DOI=10.1016/j.vetmic.2011.10.017
Brosnahan A.J., Brown D.R.
Porcine IPEC-J2 intestinal epithelial cells in microbiological investigations.
Vet. Microbiol. 156:229-237(2012)

PubMed=22193509; DOI=10.1007/s10616-011-9420-3
Steube K.G., Koelz A.-L., Uphoff C.C., Drexler H.G., Kluess J., Steinberg P.
The necessity of identity assessment of animal intestinal cell lines: a case report.
Cytotechnology 64:373-378(2012)

DOI=10.1155/2013/980651
Stoy A.C.F., Heegaard P.M.H., Sangild P.T., Ostergaard M.V., Skovgaard K.
Gene expression analysis of the IPEC-J2 cell line: a simple model for the inflammation-sensitive preterm intestine.
ISRN Genomics 2013:980651.1-980651.7(2013)

PubMed=24260272; DOI=10.1371/journal.pone.0079643
Zakrzewski S.S., Richter J.F., Krug S.M., Jebautzke B., Lee I.-F.M., Rieger J., Sachtleben M., Bondzio A., Schulzke J.-D., Fromm M., Gunzel D.
Improved cell line IPEC-J2, characterized as a model for porcine jejunal epithelium.
PLoS ONE 8:E79643-E79643(2013)

PubMed=25407967; DOI=10.1186/s12985-014-0193-0
Yan M.-F., Zhu L.-Q., Yang Q.
Infection of porcine circovirus 2 (PCV2) in intestinal porcine epithelial cell line (IPEC-J2) and interaction between PCV2 and IPEC-J2 microfilaments.
Virol. J. 11:193.1-193.9(2014)

PubMed=26147118; DOI=10.1371/journal.pone.0132323
Nossol C., Barta-Boszormenyi A., Kahlert S., Zuschratter W., Faber-Zuschratter H., Reinhardt N., Ponsuksili S., Wimmers K., Diesing A.-K., Rothkotter H.-J.
Comparing two intestinal porcine epithelial cell lines (IPECs): morphological differentiation, function and metabolism.
PLoS ONE 10:E0132323-E0132323(2015)

PubMed=29787056; DOI=10.1007/978-3-319-16104-4_12
Vergauwen H.
The IPEC-J2 cell line.
(In) The impact of food bioactives on health. In vitro and ex vivo models; Verhoeckx K., Cotter P., Lopez-Exposito I., Kleiveland C., Lea T., Mackie A., Requena T., Swiatecka D., Wichers H. (eds.); pp.125-134; Springer; Cham (2015)

PubMed=26651362; DOI=10.1021/acs.molpharmaceut.5b00874
Saaby L., Helms H.C.C., Brodin B.
IPEC-J2 MDR1, a novel high-resistance cell line with functional expression of human P-glycoprotein (ABCB1) for drug screening studies.
Mol. Pharm. 13:640-652(2016)

PubMed=26975772; DOI=10.4014/jmb.1512.12075
Kim S.H., Pajarillo E.A.B., Balolong M.P., Lee J.Y., Kang D.-K.
Constructing proteome reference map of the porcine jejunal cell line (IPEC-J2) by label-free mass spectrometry.
J. Microbiol. Biotechnol. 26:1124-1131(2016)

PubMed=32708885; DOI=10.3390/pharmaceutics12070673
Saaby L., Trasborg J., Rasmussen M.A., Holst B., Brodin B.
IPEC-J2 rMdr1a, a new cell line with functional expression of rat P-glycoprotein encoded by rat Mdr1a for drug screening purposes.
Pharmaceutics 12:673.1-673.17(2020)

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