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GM12878 人B淋巴细胞
英文名:GM12878
货号:ZQ1088
价格:¥2500.00
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推荐组合

GM12878 人B淋巴细胞

¥2500.00
+

GM12878 人B淋巴细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥2850 ¥4480.00
加入购物车
  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

GM12878 人B淋巴细胞

货号

ZQ1088

产品介绍

GM12878是一种人B淋巴细胞系,源自一位白人女性的外周血B淋巴细胞,通过EB病毒转化获得。这些细胞在生物医学研究中被广泛使用,尤其是在基因组学、表观遗传学和免疫学研究领域。 

注意事项
该细胞为悬浮细胞,建议传代时使用【半换液法】对细胞状态有利。可以直接向培养瓶中添加等体积的新鲜培养液,然后将细胞吹打均匀后移入两个新的T25培养瓶中继续培养即可(1:2传代)。

种属

性别/年龄

女/***

组织

外周血

疾病

常态 

细胞类型

转化细胞

形态学

淋巴母细胞

生长方式

悬浮

培养基和添加剂

RPMI-1640(品牌:中乔新舟 货号:ZQ-200+10%胎牛血清(中乔新舟  货号:ZQ500-A+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM1088

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37℃

STR位点信息

***

抗原表达/受体表达

*** 

基因表达

*** 

保藏机构

Coriell; GM12878

供应限制

仅供科研使用


货号

ZQ1088

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用

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Global variation in copy number in the human genome.
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PubMed=19043577; DOI=10.1371/journal.pgen.1000287
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Genetic analysis of human traits in vitro: drug response and gene expression in lymphoblastoid cell lines.
PLoS Genet. 4:E1000287-E1000287(2008)


PubMed=20856902; DOI=10.1371/journal.pbio.1000480
Cheung V.G., Nayak R.R., Wang I.X.-R., Elwyn S., Cousins S.M., Morley M., Spielman R.S.
Polymorphic cis- and trans-regulation of human gene expression.
PLoS Biol. 8:e1000480.1-e1000480.14(2010)


PubMed=21418647; DOI=10.1186/gb-2011-12-3-r25
Morcos L., Ge B., Koka V., Lam K.C.L., Pokholok D.K., Gunderson K.L., Montpetit A., Verlaan D.J., Pastinen T.
Genome-wide assessment of imprinted expression in human cells.
Genome Biol. 12:R25.1-R25.14(2011)


PubMed=23325432; DOI=10.1101/gr.147942.112
Varley K.E., Gertz J., Bowling K.M., Parker S.L., Reddy T.E., Pauli-Behn F., Cross M.K., Williams B.A., Stamatoyannopoulos J.A., Crawford G.E., Absher D.M., Wold B.J., Myers R.M.
Dynamic DNA methylation across diverse human cell lines and tissues.
Genome Res. 23:555-567(2013)


PubMed=23676674; DOI=10.1038/nature12223
Wu L.-F., Candille S.I., Choi Y., Xie D., Jiang L.-H., Li-Pook-Than J., Tang H., Snyder M.P.
Variation and genetic control of protein abundance in humans.
Nature 499:79-82(2013)


PubMed=24185094; DOI=10.1038/nbt.2728
Selvaraj S., Dixon J.R., Bansal V., Ren B.
Whole-genome haplotype reconstruction using proximity-ligation and shotgun sequencing.
Nat. Biotechnol. 31:1111-1118(2013)


PubMed=24380390; DOI=10.1186/gb-2013-14-12-r148
Schwalie P.C., Ward M.C., Cain C.E., Faure A.J., Gilad Y., Odom D.T., Flicek P.
Co-binding by YY1 identifies the transcriptionally active, highly conserved set of CTCF-bound regions in primate genomes.
Genome Biol. 14:R148.1-R148.15(2013)


PubMed=26719794; DOI=10.1186/s13742-015-0106-1
Teo A.S.M., Verzotto D., Yao F., Nagarajan N., Hillmer A.M.
Single-molecule optical genome mapping of a human HapMap and a colorectal cancer cell line.
GigaScience 4:65.1-65.6(2015)


PubMed=26621101; DOI=10.1016/j.jmoldx.2015.08.005
Pratt V.M., Everts R.E., Aggarwal P., Beyer B.N., Broeckel U., Epstein-Baak R., Hujsak P., Kornreich R., Liao J., Lorier R., Scott S.A., Smith C.-Y.H., Toji L.H., Turner A., Kalman L.V.
Characterization of 137 genomic DNA reference materials for 28 pharmacogenetic genes: a GeT-RM collaborative project.
J. Mol. Diagn. 18:109-123(2016)


PubMed=27792722; DOI=10.1371/journal.pcbi.1005151
Dilthey A.T., Gourraud P.-A., Mentzer A.J., Cereb N., Iqbal Z., McVean G.A.T.
High-accuracy HLA type inference from whole-genome sequencing data using population reference graphs.
PLoS Comput. Biol. 12:e1005151.1-e1005151.16(2016)



CLPUB00604

Chow S.
Targeted capture and sequencing of immunoglobulin rearrangements in multiple myeloma to enable detection of minimal residual disease.
Thesis MSc (2017), University of Toronto, Canada



PubMed=29116076; DOI=10.1038/s41467-017-01467-7
Garieri M., Delaneau O., Santoni F.A., Fish R.J., Mull D., Carninci P., Dermitzakis E.T., Antonarakis S.E., Fort A.
The effect of genetic variation on promoter usage and enhancer activity.
Nat. Commun. 8:1358.1-1358.9(2017)


PubMed=29431738; DOI=10.1038/nbt.4060
Jain M., Koren S., Miga K.H., Quick J., Rand A.C., Sasani T.A., Tyson J.R., Beggs A.D., Dilthey A.T., Fiddes I.T., Malla S., Marriott H., Nieto T., O'Grady J., Olsen H.E., Pedersen B.S., Rhie A., Richardson H., Quinlan A.R., Snutch T.P., Tee L., Paten B., Phillippy A.M., Simpson J.T., Loman N.J., Loose M.W.
Nanopore sequencing and assembly of a human genome with ultra-long reads.
Nat. Biotechnol. 36:338-345(2018)


PubMed=30485824; DOI=10.1016/j.celrep.2018.10.096
Papp E., Hallberg D., Konecny G.E., Bruhm D.C., Adleff V., Noe M., Kagiampakis I., Palsgrove D., Conklin D., Kinose Y., White J.R., Press M.F., Drapkin R.I., Easwaran H., Baylin S.B., Slamon D.J., Velculescu V.E., Scharpf R.B.
Integrated genomic, epigenomic, and expression analyses of ovarian cancer cell lines.
Cell Rep. 25:2617-2633(2018)


PubMed=31273215; DOI=10.1038/s41597-019-0116-4
Osorio D., Yu X., Yu P., Serpedin E., Cai J.J.
Single-cell RNA sequencing of a European and an African lymphoblastoid cell line.
Sci. Data 6:112-112(2019)


PubMed=31401124; DOI=10.1016/j.jmoldx.2019.06.007
Gaedigk A., Turner A., Everts R.E., Scott S.A., Aggarwal P., Broeckel U., McMillin G.A., Melis R., Boone E.C., Pratt V.M., Kalman L.V.
Characterization of reference materials for genetic testing of CYP2D6 alleles: a GeT-RM collaborative project.
J. Mol. Diagn. 21:1034-1052(2019)


PubMed=36094314; DOI=10.1128/JVI.00739-22
Ding W.-Y., Wang C., Narita Y., Wang H.-B., Leong M.M.L., Huang A., Liao Y.-F., Liu X.-F., Okuno Y., Kimura H., Gewurz B.E., Teng M.-X., Jin S.-L., Sato Y., Zhao B.
The Epstein-Barr virus enhancer interaction landscapes in virus-associated cancer cell lines.
J. Virol. 96:e0073922.1-e0073922.11(2022)

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