产品名称 |
NCI-H526人小细胞肺癌细胞 |
货号 |
ZQ1090 |
产品介绍 |
NCI-H526是一种人小细胞肺癌(SCLC)细胞系,源自一位55岁白人男性的肺部肿瘤。这个细胞系在癌症研究中有着广泛的应用,尤其是在小细胞肺癌的研究领域。据报道NCI-H526细胞中2 种小细胞肺癌生化标志物:神经元特异性烯醇化酶和脑型肌酸激酶同工酶表达水平较高。不表达左旋多巴羧化酶或蛙皮素(bombesin)样免疫反应性。这些细胞表达c-kit基因以及N-myc基因,但不表达 c-myc、L-myc。N-myc被放大并观察到p75 c-myb 表达。NCI-H526还表达原癌基因 N-ras、Ki-ras、Ha-ras和c-raf1。仅检测到微量的视网膜母细胞瘤易感基因 RB mRNA。未检测到RB蛋白。与正常肺中的表达水平相比,该细胞p53 mRNA表达水平较高。存在异常大小的mRNA。据报道,该细胞在软琼脂糖中的集落形成效率为4.2%。 注意事项:该细胞以大量聚团形式悬浮生长,无法测算存活率,日常培养时培养液中通常含有大量细胞碎片,几乎无法去除,是正常现象,也可在培养液中添加双抗,预防细菌污染。 |
种属 |
人 |
性别/年龄 |
男/55岁 |
组织 |
肺 |
疾病 |
肺癌 |
细胞类型 |
肿瘤细胞 |
形态学 |
悬浮、多细胞团簇 |
生长方式 |
悬浮 |
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
STR位点信息 |
Amelogenin: X,Y |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-5811 KCLB; 90526 |
供应限制 |
仅供科研使用 |
货号 |
ZQ1090 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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