产品名称 |
A-427人肺癌细胞 |
货号 |
ZQ1111 |
产品介绍 |
A-427是一种人肺腺癌细胞系,源自一位52岁白人男性的肺癌细胞,由Giard·D·J建立。这些细胞在癌症研究中被广泛使用,具有上皮形态并贴壁生长。尤其是在肺癌的生物学特性研究、药物筛选和病理机制研究方面。在第 60 代,A427 细胞显示出亚三倍体至超三倍体的核型。这意味着细胞具有异常的染色体,包括双着丝粒、微小着丝粒和大的亚端着丝粒标记。这种核型异常通常与癌细胞有关,并导致该细胞系具有独特的特征。 A427 细胞具有致瘤特性,因此当注射到裸鼠体内时,它们会形成肿瘤。
|
种属 |
人 |
性别/年龄 |
男/52岁 |
组织 |
肺 |
疾病 |
肺癌 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮 |
生长方式 |
贴壁 |
倍增时间 |
28 hours (Note=In RPMI 1640 + 10% FBS), 38 hours (Note=In ACL-3), 42 hours (Note=In ACL-3+BSA) (PubMed=3940644); 36.6 hours (PubMed=29681454); ~60-70 hours (DSMZ=ACC-234) |
培养基和添加剂 |
MEM(含有NEAA)(品牌:中乔新舟 货号:ZQ-300)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
ZM1111 |
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
STR位点信息 |
Amelogenin :X,Y CSF1PO :10,12 D2S1338: 17 D3S1358: 16 D5S818: 12 D7S820: 8,12 D8S1179: 12,13 D13S317: 11,12 D16S539: 11,13 D18S51: 12 D19S433: 13,14 D21S11: 31.2 FGA: 18 Penta D: 13 Penta E: 15,17 TH01 :9 TPOX :8,11 vWA :17 |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-7906ATCC; HTB-53 DSMZ; ACC-234 |
供应限制 |
仅供科研使用 |
货号 |
ZQ1111 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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