产品名称 |
NCI-H82 人小细胞肺癌细胞 |
货号 |
ZQ1159 |
产品介绍 |
NCI-82细胞系是由A.F.所衍生。1978年,从一个肺小细胞癌患者的胸腔液中取出了GARD和相关物。原瘤形态不具有SCLC特征。这条线是SCLC表达神经元特异性烯醇化酶和肌酸激酶脑同工酶的生化和形态变异。它没有可检测到的L-DOPA脱羧酶或子蛋白水平。细胞产生异常大的p53mrna(3.7kb)。c-mycDNA序列被放大了大约25倍,与正常细胞相比,c-mycRNA增加了24倍。据报道,这些细胞表达的是功能性的ANP受体,但是用ANP治疗不会改变它们的生长模式。这些细胞对神经丝和维门汀呈阳性染色。表达v-fes,v-fms,Ha-ras,Ki-ras,N-ras和c-raf 1 mRNA。
注意事项: |
种属 |
人 |
性别/年龄 |
男,40岁 |
组织 |
胸腔积液 |
疾病 |
小细胞肺癌 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮 |
生长方式 |
悬浮,在悬浮体中聚集,细胞以非常大的聚集体增长,而聚集体是唯一可存活的细胞群 |
倍增时间 |
24 hours (PubMed=25984343); ~25 hours (CLS=300442); ~30 hours (DSMZ=ACC-556) |
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) +1% 丙酮酸钠(中乔新舟 货号:CSP003)+ 1% L-谷氨酰胺(中乔新舟 货号:CSP012) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
STR位点信息 |
Amelogenin: X
CSF1PO: 11 |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; HTB-175 CLS; 300442 DSMZ; ACC-556 KCLB; 30175 |
供应限制 |
仅供科研使用 |
货号 |
ZQ1159 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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