产品名称 |
GP2d 人结肠癌细胞 |
货号 |
ZQ1170 |
产品介绍 |
GP2d已经从与GP5d相同的腺癌(ECACC目录号:95090715)。ECACC的STR分析证实了这一点。这些细胞来源于一位71岁女性手术切除后局部复发的Duke B级低分化结肠癌。患者术前未接受化疗或放疗,有强烈的结肠癌家族史,两名一级亲属在55岁以下死于结肠癌。这两个克隆都具有与结肠癌肿瘤进展模式一致的遗传变化,但在形态上是不同的。据报道,5号染色体(14q至22q区)间质缺失和10q11和10q21带的反向重复。这两种细胞系都含有ki-ras基因的正常拷贝和密码子12转移的拷贝。随着细胞数量的增加,GP2d细胞对EGF、TGF α或胰岛素表现出生长反应。双调节蛋白mRNA在GP2d中含量丰富,而在GP5d中几乎检测不到。 |
种属 |
人 |
性别/年龄 |
女/71岁 |
组织 |
结肠 |
疾病 |
结肠癌 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮 |
生长方式 |
贴壁 |
倍增时间 |
大约27~32小时 |
培养基和添加剂 |
DMEM (中乔新舟 货号:ZQ-100)+10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
STR位点信息 |
Amelogenin: X |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ECACC; 95090714 |
供应限制 |
仅供科研使用 |
货号 |
ZQ1170 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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