产品名称 |
SNU-398人肝癌细胞 |
货号 |
ZQ1192 |
产品介绍 |
SNU-398 由 J.-G. 于 1990 年衍生。 Park 及其同事研究了一位韩国患者的间变性肝细胞癌,该患者接受了脂质醇联合阿霉素和丝裂霉素 C 的经导管动脉栓塞治疗。 肿瘤细胞最初在补充有5%热灭活胎牛血清的ACL-4培养基中培养。建立后,将培养物维持在补充有10%热灭活胎牛血清的RPMI 1640中。 大体上,原发肿瘤呈单结节状,有结节周围延伸。组织学上为小梁型。培养的细胞是多核的,并维持细胞质内透明球的产生,如原始肿瘤中所见。通过Southern印迹杂交检测乙型肝炎病毒(HBV)DNA。HBV基因组RNA未表达。 |
种属 |
人 |
性别/年龄 |
男,42岁 |
组织 |
肝 |
疾病 |
肝癌 |
细胞类型 |
肿瘤细胞细胞系 |
形态学 |
上皮细胞样 |
生长方式 |
半贴壁半悬浮 |
倍增时间 |
~25.49-39 hours |
培养基和添加剂 |
RPMI-1640(品牌:中乔新舟 货号:ZQ-200) +10%胎牛血清(中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
ZM1192 |
生物安全等级 |
BSL-2 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
STR位点信息 |
Amelogenin: X,Y CSF1PO: 13 D2S1338: 19 D3S1358: 17 D5S818: 12 D7S820: 10,11 D8S1179: 12 D13S317: 11 D16S539: 10,14 D18S51: 15 (PubMed=25877200) 15,19 (ATCC=CRL-2233) D19S433: 14,14.1 D21S11: 29 FGA: 22,23 (KCLB=00398) 23 (ATCC=CRL-2233; PubMed=25877200) Penta D: 14 Penta E: 12 TH01: 7,9 (ATCC=CRL-2233; Cosmic-CLP=1240217; PubMed=25877200) 8,9 (KCLB=00398) 9 (PubMed=31378681) TPOX: 11 vWA: 17,18 |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-2233 KCLB; 00398 |
供应限制 |
仅供科研使用 |
货号 |
ZQ1192 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=7543080; DOI=10.1002/ijc.2910620308
Park J.-G., Lee J.-H., Kang M.-S., Park K.-J., Jeon Y.-M., Lee H.-J., Kwon H.-S., Park H.-S., Yeo K.-S., Lee K.-U., Kim S.-T., Chung J.-K., Hwang Y.-J., Lee H.-S., Kim C.Y., Lee Y.I., Chen T.-R., Hay R.J., Song S.-Y., Kim W.-H., Ki C.-W., Kim Y.-I.
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Biology of SNU cell lines.
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