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名称 |
MAC-T牛乳腺上皮细胞 |
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货号 |
ZQ1204 |
|
产品介绍 |
通过稳定转染猿猴病毒SV40大T抗原来开发 MAC-T 牛乳腺上皮细胞系，应极大地有助于研究调节牛乳腺上皮细胞发育、分化和功能的可能内源性（局部）和外源性（内吸性）因素。 |
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种属 |
牛 |
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组织来源 |
乳腺 |
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形态学 |
上皮细胞样 |
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细胞类型 |
转化细胞系 |
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倍增时间 |
~17-48 hours |
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生长方式 |
贴壁 |
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培养基和添加剂 |
DMEM (中乔新舟 货号：ZQ-100)+10%胎牛血清（中乔新舟 货号：ZQ500-A）+1%P/S（中乔新舟 货号：CSP006） |
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推荐完全培养基货号 |
ZM1204 |
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生物安全等级 |
BSL-2 |
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培养条件 |
95%空气，5%二氧化碳；37℃ |
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保藏机构 |
BCRJ; 0315 |
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供应限制 |
仅供科研使用 |
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货号 |
ZQ1204 |
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发货规格 |
活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一 |
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发货形式 |
活细胞：常温运输；冻存管：干冰运输 |
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储存温度 |
活细胞：培养箱；冻存管：液氮罐 |
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产地 |
中国 |
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供应限制 |
仅供科研使用 |
CLPUB00665
Huynh H.T.
Establishment of bovine mammary epithelial cell lines: an in vitro model for lactation.
Thesis MSc (1990); McGill University Montreal; Montreal; Canada
PubMed=1659986; DOI=10.1016/0014-4827(91)90422-Q
Huynh H.T., Robitaille G., Turner J.D.
Establishment of bovine mammary epithelial cells (MAC-T): an in vitro model for bovine lactation.
Exp. Cell Res. 197:191-199(1991)
PubMed=1423654; DOI=10.1016/S0309-1651(06)80052-4
Rejman J.J., Oliver S.P., Muenchen R.A., Turner J.D.
Proliferation of the MAC-T bovine mammary epithelial cell line in the presence of mammary secretion whey proteins.
Cell Biol. Int. Rep. 16:993-1001(1992)
Patent=US5227301
Turner J.D., Huynh H.T.
Immortalized bovine mammary epithelial cell line.
Patent number US5227301, 13-Jul-1993
Patent=US5455164
Turner J.D.
Ruminant immortalized mammary epithelial cell lines.
Patent number US5455164, 03-Oct-1995
PubMed=7540186; DOI=10.3168/jds.S0022-0302(95)76662-0
Zavizion B., Gorewit R.C., Politis I.
Subcloning the MAC-T bovine mammary epithelial cell line: morphology, growth properties, and cytogenetic analysis of clonal cells.
J. Dairy Sci. 78:515-527(1995)
PubMed=14550508; DOI=10.1016/S0739-7240(03)00062-6
Berry S.D.K., Weber Nielsen M.S., Sejrsen K., Pearson R.E., Boyle P.L., Akers R.M.
Use of an immortalized bovine mammary epithelial cell line (MAC-T) to measure the mitogenic activity of extracts from heifer mammary tissue: effects of nutrition and ovariectomy.
Domest. Anim. Endocrinol. 25:245-253(2003)
DOI=10.4172/2329-888X.1000103
Hosseini A., Sharma R., Bionaz M., Loor J.J.
Transcriptomics comparisons of Mac-T cells versus mammary tissue during late pregnancy and peak lactation.
Adv. Dairy Res. 1:103-103(2013)
PubMed=26659725; DOI=10.1152/ajpcell.00261.2015
Arevalo Turrubiarte M., Perruchot M.-H., Finot L., Mayeur F., Dessauge F.
Phenotypic and functional characterization of two bovine mammary epithelial cell lines in 2D and 3D models.
Am. J. Physiol. Cell Physiol. 310:C348-C356(2016)
PubMed=30055215; DOI=10.1016/j.virusres.2018.07.013
Martinez Cuesta L., Nieto Farias M.V., Lendez P.A., Barone L., Perez S.E., Dolcini G.L., Ceriani M.C.
Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV).
Virus Res. 256:11-16(2018)
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