产品名称 |
LS1034人结肠腺癌细胞 |
货号 |
ZQ1217 |
产品介绍 |
LS1034是1989年从一名54岁白人男性患者的原发肿瘤活检中分离出的一种结直肠癌细胞系,该患者被诊断为Dukes C型,中分化至低分化盲肠癌。 超过90%的LS1034细胞表达表面CEA。 这些细胞表达主要组织相容性复合体(MHC)I类抗原和β2微球蛋白,但未检测到II类抗原(HLA-DR、DQ和DP)。 没有可测量的潜伏性转化生长因子β-1 (TGFβ-1) 分泌。 皮克摩尔浓度的TGF beta-1、TGF beta-2和TGF beta-3抑制LS1034细胞的增殖。 LS1034细胞对多种药物具有耐药性(MDR),并且可以在无血清培养基中增殖。 患者没有表现出家族性腺瘤息肉病(FAP),正常组织中没有APC基因的缺失。 在含有5%胎牛血清的甲基纤维素培养基中,克隆形成效率为3%,在无血清的甲基纤维素培养基中,与含有血清的半固体培养基相比,克隆生成性增加了600%。 |
种属 |
人 |
性别/年龄 |
男,54岁 |
组织 |
盲肠 |
疾病 |
盲肠腺癌 |
细胞类型 |
肿瘤细胞系 |
形态学 |
成肌细胞样 |
生长方式 |
贴壁 |
倍增时间 |
~35 huors |
培养基和添加剂 |
RPMI-1640(中乔新舟 货号:ZQ-200) +10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
ZM1217 |
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
STR位点信息 |
Amelogenin X,Y CSF1PO 11,12 D2S1338 17,25 D3S1358 15,18 D5S818 12,13 D7S820 8,12 D8S1179 13 D13S317 12 D16S539 9,11 D18S51 20 D19S433 13,15 D21S11 29,30 FGA 20 Penta D 11,13 Penta E 5,13 TH01 7 TPOX 9,11 vWA 17 |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-2158 BCRC; 60353 KCLB; 22158 |
供应限制 |
仅供科研使用 |
货号 |
ZQ1217 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*2支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
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