产品名称	Bewo人胎盘绒膜癌细胞
货号	ZQ0448
产品介绍	BeWo细胞是一种来源于胎儿男性胎盘恶性妊娠绒毛膜癌的细胞系，已成为广泛应用于胎盘研究的体外模型。胎盘发育过程中滋养细胞合胞期的细胞-细胞融合是最重要，但却鲜为人知的事件之一。由于这一过程难以在体内胎盘中进行研究，我们利用BeWo细胞作为细胞培养模型来模拟胎盘绒毛滋养细胞的体内合胞过程。 该细胞表现出上皮样表型和粘附性。BeWo细胞的b30亚克隆由于其在透膜上的密集生长，对研究营养物质的吸收和运输特别有用。ck7和E-cadherin是BeWo细胞表达的分子标记物。ve -钙粘蛋白存在于BeWo细胞中，并在用forskolin处理后增强。这些细胞也表达角蛋白，并且G6PD, B同工酶呈阳性。BeWo细胞的核型为模态数= 86，范围为71 ~ 178，茎系数为准四倍体。 核型在茎系数内相对稳定。BeWo细胞分泌各种激素，包括人绒毛膜促性腺激素(hCG)、人绒毛膜促生长激素(胎盘乳原)和类固醇激素，如雌酮、雌三醇和雌二醇。但BeWo细胞分泌的β-hCG和雌二醇水平低于JEG-3等其他绒毛膜癌衍生细胞系。经Forskolin处理后，BeWo细胞中β-hCG的分泌增加到与其他绒毛膜癌衍生细胞系相似的水平。此外，Forskolin处理也增加了BeWo细胞分泌的孕酮水平。 综上所述，BeWo细胞是一种广泛用于研究胎盘发育和人滋养细胞合胞过程的体外模型。它们表现出上皮样表型，表达多种分子标记，分泌多种激素，包括hCG、胎盘乳原和类固醇激素。总之，BeWo细胞是研究涉及胎盘发育的复杂过程的有价值的工具。
种属	人
性别/年龄	男
组织	胎盘
疾病	绒膜癌
细胞类型	肿瘤细胞
形态学	上皮的
生长方式	贴壁
倍增时间	大约30小时
培养基和添加剂	F12K基础培养基（中乔新舟  货号：ZQ-599）+15%胎牛血清（中乔新舟 货号：ZQ0500）+1%P/S（中乔新舟  货号：CSP006）
推荐完全培养基货号	ZM0448
生物安全等级	BSL-1
STR位点信息	Amelogenin: X,Y CSF1PO: 11,12 D13S317: 9,11 D16S539: 13,14 D5S818: 10,11 D7S820: 10,12 THO1: 9,9.3 TPOX: 8 vWA: 16
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达	***
基因表达	***
保藏机构	ATCC; CCL-98 DSMZ; ACC-458 ECACC; 86082803
供应限制	仅供科研使用

货号	ZQ0448
发货规格	活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一
发货形式	活细胞：常温运输；冻存管：干冰运输
储存温度	活细胞：培养箱；冻存管：液氮罐
产地	中国
供应限制	仅供科研使用

论文标题： LncRNA TDRG1/miR‐214‐5p axis affects preeclampsia by modulating trophoblast cells
DOI： 10.1002/cbf.3480
发表时间： 2019-12-29
期刊： CELL BIOCHEMISTRY AND FUNCTION
影响因子： 2.142
货号： ZQ0448
产品名称： BeWo cells

原文链接： https://onlinelibrary.wiley.com/doi/abs/10.1002/cbf.3480

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