人支气管上皮细胞HBEpiC

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人支气管上皮细胞

英文名：Human Bronchial Epithelial Cells
货号：3210
价格：￥14220.00

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人支气管上皮细胞说明：

呼吸道上皮细胞是由纤毛、无纤毛和能分泌黏液的细胞按照从近端到远端呼吸道分布的混合细胞群组成。这类细胞的各个亚型细胞各具特点，从而不仅能形成物理屏障，可有效的防止多种有毒物质，而且通过产生和分泌大量化学介质和细胞因子形成高度复杂的宿主防御系统。支气管上皮由表面的上皮细胞和粘液腺体组成。表面的上皮细胞由基细胞，杯状细胞和纤毛细胞三种细胞构成，形成了基底柱状结构，并能清除粘液纤毛。用支气管上皮细胞培养分化诱导纤毛表型的研究显示，IL-4和IL-13的刺激能改变增殖、纤毛运动、黏液分泌等一系列细胞功能。支气管上皮细胞增殖以及其它细胞过程也部分受到EGF受体信号的调节。支气管上皮细胞的培养为我们研究这类细胞的功能和病理生理机制提供了一个体外模型。

人支气管上皮细胞(HBEC)提取于人肺组织，原代冻存。每管含有细胞数>5×105cells/ml，此细胞通过Cytokeratin-18, -19和Vimentin抗体荧光免疫方法验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。

推荐培养基: 支气管上皮细胞培养基 (BECM, Cat. No. 3211)

产品使用说明：仅供科研研究使用

货号	3210
产地	美国
缩写	HBEpiC
规格	5 x 10^5 cells/vial
用途	科研
运输	干冰
保存	液氮

1.) Crowley, C., Klanrit, P., Butler, C.R., Varanou, A., Plat�, M., Hynds, R.E., Chambers, R.C., Seifalian, A.M., Birchall, M.A. & Janes, S.M. (2016) Surface modification of a POSS-nanocomposite material to enhance cellular integration of a synthetic bioscaffold Biomaterials. 83

2.) Bai, X.Y., Zhang, X.C., Yang, S.Q., An, S.J., Chen, Z.H., Su, J., Xie, Z., Gou, L.Y. & Wu, Y.L. (2016) Blockade of Hedgehog Signaling Synergistically Increases Sensitivity to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitors in Non-Small-Cell Lung Cancer Cell Lines PLoS One. 11

3.) Zhan, W., Han, T., Zhang, C., Xie, C., Gan, M., Deng, K., Fu, M. & Wang, J.B. (2015) TRIM59 Promotes the Proliferation and Migration of Non-Small Cell Lung Cancer Cells by Upregulating Cell Cycle Related Proteins PLoS One. 10

4.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19

5.) Shilo K, Wu X, Sharma S, Welliver M, Duan W, Villalona-Calero M, Fukuoka J, Sif S, Baiocchi R, Hitchcock CL, Zhao W, Otterson GA. (2013) 'Cellular localization of protein arginine methyltransferase-5 correlates with grade of lung tumors.'

6.) Shilo K, Wu X, Sharma S, Welliver M, Duan W, Villalona-Calero M, Fukuoka J, Sif S, Baiocchi R, Hitchcock CL, Zhao W, Otterson GA. (2013) "Cellular localization of protein arginine methyltransferase-5 correlates with grade of lung tumors." Diagnpathol.8: 201.

7.) Jia R, Li C, McCoy JP, Deng CX, Zheng ZM. (2010) "SRp20 is a proto-oncogene critical for cell proliferation and tumor induction and maintenance." Int J Biol Sci. 6: 806-26.

8.) Jiang H, Zhu YS, Xu H, Sun Y, Li QF. (2010) "Inflammatory stimulation and hypoxia cooperatively activate HIF-1{alpha} in bronchial epithelial cells: involvement of PI3K and NF-{kappa}B." Am J Physiol Lung Cell Mol Physiol. 298: L660-9.

9.) Wong CK, Cao J, Yin YB, Lam CW. (2010) "Interleukin-17A activation on bronchial epithelium and basophils: a novel inflammatory mechanism." Eur Respir J. 35: 883-93.

10.) Crawford M, Batte K, Yu L, Wu X, Nuovo GJ, Marsh CB, Otterson GA, Nana-Sinkam SP. (2009) "MicroRNA 133B targets pro-survival molecules MCL-1 and BCL2L2 in lung cancer." Biochem Biophys Res Commun. 388: 483-9.

11.) Lleo A, Selmi C, Invernizzi P, Podda M, Coppel RL, Mackay IR, Gores GJ, Ansari AA, Van de Water J, Gershwin ME. (2009) "Apotopes and the biliary specificity of primary biliary cirrhosis." Hepatology. 49: 871-9.

12.) Crawford M, Brawner E, Batte K, Yu L, Hunter MG, Otterson GA, Nuovo G, Marsh CB, Nana-Sinkam SP. (2008) "MicroRNA-126 inhibits invasion in non-small cell lung carcinoma cell lines." Biochem Biophys Res Commun. 373: 607-12.

Question:1

Why HBEpics are not named NHBE cells (NHBE is used to term " normal human bronchial epithelial cells" in literature )? Are HBEpics mixture of three kinds of cells? Can HBEpics be cultured in BEGM media (this media is used to culture NHBE in literature)?
Answers:
Posted by Karishma Shah on Tuesday, June 21, 2016
Dear customer,
HBEpiC is how ScienCell name human bronchial epithelial cells, which contain only epithelial cells from bronchia. The cells are cultured in ScienCell Bronchial Epithelial Cell medium right after isolation. Therefore, customers need to purchase ScienCell corresponding medium for culturing cells.

Question:2
HBEpics (Human Bronchial Epithelial Cells, cat no.3210 ) are NHBE cells.
Answers:
Posted by Manoj Sharma on Wednesday, April 20, 2016
Hi,
All our cells are isolated from Normal tissues, so to answer your question.
YES, Human Bronchial Epithelial Cells are NHBE cells
Thanks you

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