人缺氧应答定量PCR芯片试剂盒描述：
Hypoxia refers to any environment with reduced oxygen content compared to normal aerobic conditions. The hypoxic response is induced when cells are oxygen deprived and prolonged deprivation often results in physiological compensation by the local cellular environment, such as increased angiogenesis to the hypoxic region. ScienCell's
GeneQuery™ Human Hypoxia Response qPCR Array Kit (GQH-HPX) is designed to facilitate gene expression profiling of 88 key genes involved in the cellular response to low-oxygen conditions. Brief examples of how included genes may be grouped according to response type are shown below:
Cellular metabolism: ENO1, HK1, PFKFB3, PGK1, SLC2A1
Inflammation: CXCL8, IL1A, CCL2, IL6, THBD
Vasorelaxation: AHSP, PTGIS
Cell death or proliferation: BNIP3L, NOS3, PIM1, EPO
Angiogenesis: KDR, PDGFB, PLAU, TEK, FLT1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Endothelial Cell Biology qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
AHSP |
BNIP3L |
EGLN1 |
EPO |
HIF3A |
JMJD6 |
OS9 |
PIK3CA |
PTGIR |
SAT2 |
TEK |
β-actin |
|
B |
ALDOA |
CA9 |
EGLN2 |
F2 |
HK1 |
KDR |
PDGFB |
PIM1 |
PTGIS |
SELP |
THBD |
GAPDH |
|
C |
ANXA2 |
CCL2 |
EGLN3 |
FLT1 |
HRH1 |
LDHA |
PDPK1 |
PLAU |
PTGS2 |
SENP1 |
TNF |
LDHA |
|
D |
APEX1 |
COPS5 |
EGR1 |
FOXO4 |
ICAM1 |
LGALS3 |
PECAM1 |
PPP3CB |
RACK1 |
SLC2A1 |
TP53 |
NONO |
|
E |
ARNT |
CREBBP |
EIF4E |
GSK3B |
IFNG |
LTC4S |
PER1 |
PPP3CC |
RPS6 |
SP1 |
TXN |
PPIH |
|
F |
ARNT2 |
CUL2 |
ENO1 |
GYS1 |
IL1A |
MAP3K1 |
PFKFB3 |
PRKCA |
RUVBL2 |
STAT3 |
USP20 |
GDC |
|
G |
ARNTL |
CXCL8 |
EP300 |
HIF1A |
IL4 |
NFKB1 |
PFKFB4 |
PSMC6 |
RWDD3 |
TCEB1 |
VCAM1 |
PPC |
|
H |
BNIP3 |
EDN1 |
EPAS1 |
HIF1AN |
IL6 |
NOS3 |
PGK1 |
PTGFR |
SAT1 |
TCEB2 |
VHL |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK035 |
|
产地 |
美国 |
|
缩写 |
GQH-HPX |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 |
| 1200 | 人脑血管周细胞 | 5 x 10^5 cells/vial | ￥14220.00 | 放入购物车 》 |
| 8000 | 人脐静脉内皮细胞 | 5 x 10^5 cells/vial | ￥5148.00 | 放入购物车 》 |
| 8020 | 人脐静脉平滑肌细胞 | 5 x 10^5 cells/vial | ￥8586.00 | 放入购物车 》 |
| 1001 | 内皮细胞培养基 | 500ML | ￥2224.00 | 放入购物车 》 |
| 1101 | 平滑肌细胞培养基 | 500ML | ￥2112.00 | 放入购物车 》 |
| 1201 | 周细胞培养基 | 500ML | ￥2208.00 | 放入购物车 》 |
| 5501 | 造血细胞培养基 | 500ML | ￥1904.00 | 放入购物车 》 |
| 5340 | 人肝巨噬细胞 | 1 x 10^6 cells/vial | ￥13176.00 | 放入购物车 》 |
| 8218 | 过氧化氢酶活性分析试剂盒 | 100 tests | ￥4368.00 | 放入购物车 》 |
| 8368 | NAD/NADH 含量检测试剂盒 | 100 Tests in 96-well plate | ￥6416.00 | 放入购物车 》 |
| 8268 | 线粒体分离试剂盒 | 100 reactions | ￥5136.00 | 放入购物车 》 |
| 8168 | 总抗氧化能力检测试剂盒 | 100 tests | ￥4752.00 | 放入购物车 》 |
| 1204 | 人大脑血管外膜细胞细胞cDNA | 20 reactions | ￥7087.00 | 放入购物车 》 |
| 8004 | 人脐静脉内皮细胞cDNA | 20 reactions | ￥7087.00 | 放入购物车 》 |
| 8024 | 人脐静脉平滑肌细胞cDNA | 20 reactions | ￥7087.00 | 放入购物车 》 |
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