人致癌基因定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Oncogenes qPCR Array Kit (GQH-ONC) surveys a panel of 88 known oncogenes to assist in cancer research. Oncogenes are genes with the potential to transform cells after a gain-of-function mutation. Brief examples of how included genes may be grouped according to function are shown below:
Transcription factors: CDX1, ELK1, ETS1, MAF, REL
Growth factor signaling: HGF, EGFR, FGFR1, PDGFB
Signal transduction: PRKCA, KRAS, LMO2, SMO, PIK3CA
Cell cycling/growth regulation: ABL1, CCND1, CDK4, HMGA2, MDM2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ Human Oncogenes qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
|
A |
ABL1 |
BCL3 |
CCND1 |
DDX6 |
EWSR1 |
HRAS |
MAFB |
MPL |
NRAS |
PPARG |
SRC |
β-actin |
|
B |
AKT1 |
BCL6 |
CCND2 |
EGFR |
FEV |
IRF4 |
MAML2 |
MYB |
NTRK1 |
PRKCA |
SS18 |
GAPDH |
|
C |
AKT2 |
BCR |
CCND3 |
ELK1 |
FGFR1 |
JUNB |
MCL1 |
MYC |
NUP214 |
PTPN11 |
STAT3 |
LDHA |
|
D |
ATF1 |
BRAF |
CDK4 |
ELK4 |
FUS |
KITLG |
MDM2 |
MYCL |
PAX8 |
RAF1 |
TET2 |
NONO |
|
E |
BAX |
CARD11 |
CDX2 |
ERBB2 |
GOPC |
KRAS |
MECOM |
MYCN |
PDGFB |
RARA |
TFG |
PPIH |
|
F |
BCL11A |
CASP8 |
CTNNB1 |
ETS1 |
HGF |
LCK |
MET |
NCOA4 |
PIK3CA |
REL |
TLX1 |
GDC |
|
G |
BCL2 |
CBLB |
DDB2 |
ETV1 |
HMGA1 |
LMO2 |
MITF |
NFKB2 |
PIM1 |
RET |
TPR |
PPC |
|
H |
BCL2L1 |
CBLC |
DDIT3 |
ETV6 |
HMGA2 |
MAF |
MOS |
NFKBIA |
PLAG1 |
SMO |
ZHX2 |
NTC |
*gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK054 |
|
产地 |
美国 |
|
缩写 |
GQH-ONC |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
| 货号 | 产品名称 | 规格 | 价格 | 指令 |
上海中乔新舟生物科技有限公司
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