名称 |
S-2 果蝇胚胎细胞 |
货号 |
ZQ0549 |
产品介绍 |
该细胞系是由I. Schneider于1969年从数百个20至24小时的胚胎中建立的;在16℃和28℃之间的任何温度下,细胞将作为松散的单层(或悬浮)生长;最适温度在22C-24C之间;这些细胞已经被证明支持疟原虫昆虫阶段的生长。。 |
种属 |
果蝇 |
组织来源 |
原位;全胚胎 |
形态学 |
|
细胞类型 |
自发永生化细胞系 |
倍增时间 |
~40 hours (DSMZ=ACC-130) |
生长方式 |
混合:悬浮液与一些松散贴壁的细胞 |
培养基和添加剂 |
Schneider`s insect medium +10%FBS+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
ATCC; CRL-1963 BCRC; 60442 CCTCC; GDC0138 DSMZ; ACC-130 KCB; KCB 200559YJ RCB; RCB1153 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0549 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
PubMed=4625067; DOI=10.1242/dev.27.2.353
Schneider I.
Cell lines derived from late embryonic stages of Drosophila melanogaster.
J. Embryol. Exp. Morphol. 27:353-365(1972)
DOI=10.1016/0020-1790(83)90040-9
Kramerov A.A., Metakovsky E.V., Polukarova L.G., Gvozdev V.A.
Glycoprotein patterns in different established cell lines of Drosophila melanogaster responding to 20-hydroxyecdysone.
Insect Biochem. 13:655-663(1983)
DOI=10.2307/3576172
Koval T.M.
Radiosensitivity of cultured insect cells: II. Diptera.
Radiat. Res. 96:127-134(1983)
PubMed=16593348; DOI=10.1073/pnas.80.15.4752
Koval T.M.
Intrinsic resistance to the lethal effects of X-irradiation in insect and arachnid cells.
Proc. Natl. Acad. Sci. U.S.A. 80:4752-4755(1983)
PubMed=8799737; DOI=10.1111/j.1365-2583.1996.tb00053.x
McIntosh A.H., Grasela J.J., Matteri R.L.
Identification of insect cell lines by DNA amplification fingerprinting (DAF).
Insect Mol. Biol. 5:187-195(1996)
DOI=10.1016/B978-012229460-0/50004-X
Echalier G.
Drosophila continuous cell lines.
(In) Drosophila cells in culture; Echalier G. (eds.); pp.129-186; Academic Press; New York (1997)
PubMed=15038778; DOI=10.1290/1543-706X(2003)039<0353:AOISRT>2.0.CO;2
Grasela J.J., McIntosh A.H.
Application of inter-simple sequence repeats to insect cell lines: identification at the clonal and tissue-specific level.
In Vitro Cell. Dev. Biol. Anim. 39:353-363(2003)
PubMed=16409117; DOI=10.1290/0412083R.1
McIntosh A.H., Grasela J.J., Popham H.J.R.
AcMNPV in permissive, semipermissive, and nonpermissive cell lines from Arthropoda.
In Vitro Cell. Dev. Biol. Anim. 41:298-304(2005)
PubMed=17417030; DOI=10.1007/978-1-59745-257-1_33
Ceriani M.F.
Basic protocols for Drosophila S2 cell line: maintenance and transfection.
Methods Mol. Biol. 362:415-422(2007)
PubMed=17890361; DOI=10.1534/genetics.107.076356
Williams B.R., Bateman J.R., Novikov N.D., Wu C.-T.
Disruption of topoisomerase II perturbs pairing in Drosophila cell culture.
Genetics 177:31-46(2007)
PubMed=23891577; DOI=10.1016/j.tiv.2013.07.007
Curtis T.M., Collins A.M., Gerlach B.D., Brennan L.M., Widder M.W., van der Schalie W.H., Vo N.T.K., Bols N.C.
Suitability of invertebrate and vertebrate cells in a portable impedance-based toxicity sensor: temperature mediated impacts on long-term survival.
Toxicol. In Vitro 27:2061-2066(2013)
PubMed=24434506; DOI=10.1016/j.ymeth.2014.01.006
Cherbas L., Gong L.
Cell lines.
Methods 68:74-81(2014)
PubMed=33389257; DOI=10.1007/s10096-020-04106-0
Wurtz N., Penant G., Jardot P., Duclos N., La Scola B.
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)
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