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Hacat人永生化表皮细胞(STR鉴定)[套餐促销]

英文名:Hacat
货号:ZQ0044
价格:¥1600.00
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Hacat人永生化表皮细胞(STR鉴定)[套餐促销]

¥1600.00
+

Hacat人永生化表皮细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥1950 ¥2080.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称 

Hacat人永生化表皮细胞 

货号 

ZQ0044 

产品介绍 

HaCaT细胞是一种人永生化表皮细胞系,源自一位62岁患有黑色素瘤男性的病灶外围正常皮肤组织。这种细胞系在体外培养后自然转化,获得了永生化特性,能够无限期地增殖而不会自然凋亡。HaCaT细胞保留了正常皮肤细胞的特性和功能,表达角蛋白、角化细胞交联外膜蛋白以及中间丝相关蛋白,这些蛋白在角质形成和皮肤细胞分化中起着重要的作用。HaCaT细胞因其在体外分化和增殖的高能力而被用于人表皮细胞角化的研究,并解决了培养寿命短和细胞系之间可能遇到的变异等问题。此外,它们也被广泛应用于皮肤生物学、免疫学、药物毒性研究、皮肤疾病研究、化妆品和药物测试等领域。

【注意事项】
该细胞传代时需要消化较长时间(5~15分钟),具体时间因胰酶浓度、效价、消化条件有所不同,请以细胞即将脱落为准。

种属 

 

性别/年龄 

男性/62岁

组织 

正常表皮细胞 ,背部、皮肤、表皮

疾病 

皮肤癌 

生物安全等级 

BSL -1 

STR位点信息 

Amelogenin:X;

CSF1PO:9,11;

D13S317:10,12;

D16S539:9,12;

D18S51:12;

D19S433:13,14;

D21S11:30.2;

D2S1338:17,25;

D3S1358:16;

D5S818:12;

D7S820:9,11;

D8S1179:14;

FGA:24;

TH01:9.3;

TPOX:11,12;

vWA:16,17;

细胞类型 

自发永生化细胞系 

形态学 

上皮样 

生长方式 

贴壁 

倍增时间 

大约28小时 

培养基和添加剂 

90%DMEM高糖(品牌:中乔新舟 货号:ZQ-100+10%FBS(品牌:中乔新舟 货号:ZQ0500+1%双抗(中乔新舟  货号:CSP006

推荐完全培养基货号 

ZM0044

培养条件 

95%空气,5%二氧化碳;37℃ 

抗原表达/受体表达 

*** 

基因表达 

***  

保藏机构 

CLS;300493

供应限制 

仅供科研使用 

货号

ZQ0044

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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DOI: 10.1016/j.ijbiomac.2021.07.002
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期刊: INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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货号: ZQ0044
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原文链接: https://www.sciencedirect.com/science/article/pii/S0141813021014380


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期刊: Regenerative Biomaterials
影响因子: 6.353
货号: ZQ0044
产品名称: HaCaT cells
原文链接: https://academic.oup.com/rb/article-abstract/doi/10.1093/rb/rbac023/6575919


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DOI: 10.3389/fphar.2021.655281
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期刊: Frontiers in Pharmacology
影响因子: 5.811
货号: ZQ0044
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原文链接: https://www.frontiersin.org/articles/10.3389/fphar.2021.655281/full


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货号: ZQ0044
产品名称: HaCaT cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/cam4.4706


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DOI: 10.1016/j.jep.2022.115066
发表时间: 2022-02-02
期刊: JOURNAL OF ETHNOPHARMACOLOGY
影响因子: 4.36
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原文链接: https://www.sciencedirect.com/science/article/pii/S0378874122001003


论文标题: Carnosine Stimulates Macrophage-Mediated Clearance of Senescent Skin Cells Through Activation of the AKT2 Signaling Pathway by CD36 and RAGE
DOI: 10.3389/fphar.2020.593832
发表时间: 2020-12-16
期刊: Frontiers in Pharmacology
影响因子: 4.225
货号: ZQ0044
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期刊: Marine Drugs
影响因子: 3.772
货号: ZQ0044
产品名称: HaCaT cells
原文链接: https://www.mdpi.com/580830


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DOI: 10.1002/jbio.201700357
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影响因子: 3.768
货号: ZQ0044
产品名称: HaCaT cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201700357


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DOI: 10.7150/ijms.60907
发表时间: 2021-07-11
期刊: International Journal of Medical Sciences
影响因子: 3.738
货号: ZQ0044
产品名称: human immortalized keratinocytes (HaCaT)
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8364443/


论文标题: Pseudoephedrine alleviates atopic dermatitis-like inflammatory responses in vivo and in vitro
DOI: 10.1016/j.lfs.2020.118139
发表时间: 2020-07-25
期刊: LIFE SCIENCES
影响因子: 3.647
货号: ZQ0044
产品名称: HaCaT cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0024320520308900


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DOI: 10.1016/j.bbrc.2018.03.207
发表时间: 2018-03-31
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子: 3.575
货号: ZQ0044
产品名称: HaCaT cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0006291X18307368


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期刊: European Review for Medical and Pharmacological Sciences
影响因子: 3.507
货号: ZQ0044
产品名称: HaCaT cells
原文链接: https://pubmed.ncbi.nlm.nih.gov/34533810/


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DOI: 10.1080/21655979.2021.1987089
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原文链接: https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.1987089


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DOI: 10.1111/1346-8138.15370
发表时间: 2020-06-11
期刊: JOURNAL OF DERMATOLOGY
影响因子: 3.072
货号: ZQ0044
产品名称: human immortalized keratinocyte cell HaCaT
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1111/1346-8138.15370


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DOI: 10.3892/ijmm.2018.3901
发表时间: 2018-09-26
期刊: INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
影响因子: 2.784
货号: ZQ0044
产品名称: HaCaT cells
原文链接: https://www.spandidos-publications.com/10.3892/ijmm.2018.3901


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DOI: 10.1111/jfbc.14163
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期刊: JOURNAL OF FOOD BIOCHEMISTRY
影响因子: 2.72
货号: ZQ0044
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影响因子: 1.621
货号: ZQ0044
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期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
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原文链接: https://www.tandfonline.com/doi/abs/10.1080/09168451.2020.1783196



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