产品名称 |
HT-29人结肠癌细胞 |
货号 |
ZQ0057 |
产品介绍 |
HT-29细胞系是1964年由福格用外植体培养法从原发肿瘤中分离出来的。HT-29细胞表达尿激酶受体,但未检测到纤溶酶原激活物活性[PubMed ID:8381394]。HT-29 细胞CD4表达阴性,但细胞表面表达半乳糖神经酰胺(一个HIV的可替代受体)。致癌基因c-myc、K-ras、H-ras、N-ras、Myb、sis、fos表达阳性,过量产生p53抗原,在p53基因的273密码子处有G->A突变导致Arg->His替换,未检测到致癌基因N-myc的表达。 |
种属 |
人 |
性别/年龄 |
女/44岁 |
组织 |
结肠 |
疾病 |
结肠直肠腺癌 |
生物安全等级 |
1 |
STR位点信息 |
Amelogenin: X CSF1PO: 11,12 D13S317: 11,12 D16S539: 11,12 D5S818: 11,12 D7S820: 10 TH01: 6,9 TPOX: 8,9 vWA: 17,19 |
细胞类型 |
|
形态学 |
上皮 |
生长方式 |
贴壁 |
倍增时间 |
|
培养基和添加剂 |
McCoy's 5A基础培养基(品牌:中乔新舟 货号:ZQ-1000)+10%FBS(品牌:中乔新舟 货号:ZQ0500)+1%PS(货号:CSP006) |
推荐完全培养基货号 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
|
基因表达 |
|
保藏机构 |
ATCC; HTB-38 BCRJ; 0111 CCLV; CCLV-RIE 0960 DSMZ; ACC-299 ECACC; 91072201 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0057 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes, lipid droplets, few primary and many secondary lysosomes.
The cells express urokinase receptors, but do not have detectable plasminogen activator activity [PubMed ID: 8381394]. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV).
The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. The p53 antigen is overproduced, and there is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. N-myc oncogene expression was not detected.
There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.
论文标题: MiR-21-5p Induces Pyroptosis in Colorectal Cancer via TGFBI
DOI: 10.3389/fonc.2020.610545
发表时间: 2021-02-05
期刊: Frontiers in Oncology
影响因子: 6.244
货号: ZQ0057
产品名称: HT-29 cells
原文链接: https://pubmed.ncbi.nlm.nih.gov/33614494/
论文标题: Knockdown of SFRS9 Inhibits Progression of Colorectal Cancer Through Triggering Ferroptosis Mediated by GPX4 Reduction
DOI: 10.3389/fonc.2021.683589
发表时间: 2021-07-16
期刊: Frontiers in Oncology
影响因子: 6.244
货号: ZQ0057
产品名称: HT29 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8322952/
论文标题: Integrating network pharmacology deciphers the action mechanism of Zuojin capsule in suppressing colorectal cancer
DOI: 10.1016/j.phymed.2021.153881
发表时间: 2021-12-09
期刊: PHYTOMEDICINE
影响因子: 5.34
货号: ZQ0057
产品名称: HT29 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0944711321004219
论文标题: PRDX2 removal inhibits the cell cycle and autophagy in colorectal cancer cells
DOI: 10.18632/aging.103690
发表时间: 2020-07-20
期刊: Aging-US
影响因子: 4.831
货号: ZQ0057
产品名称: HT29 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7485722/
论文标题: Noninvasive Evaluation of EGFR Expression of Digestive Tumors Using 99mTc-MAG3-Cet-F(ab)2-Based SPECT/CT Imaging
DOI: 10.1155/2022/3748315
发表时间: 2022-06-24
期刊: Molecular Imaging
影响因子: 4.488
货号: ZQ0057
产品名称: HT29 cells
原文链接: https://www.hindawi.com/journals/moi/2022/3748315/
论文标题: miR-524-5p inhibits angiogenesis through targeting WNK1 in colon cancer cells
DOI: 10.1152/ajpgi.00369.2019
发表时间: 2020-04-07
期刊: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
影响因子: 3.725
货号: ZQ0057
产品名称: HT-29 cells
原文链接: https://journals.physiology.org/doi/abs/10.1152/ajpgi.00369.2019
论文标题: Genes encoding bile salt hydrolase differentially affect adhesion of Lactiplantibacillus plantarum AR113
DOI: 10.1002/jsfa.11487
发表时间: 2021-08-31
期刊: JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
影响因子: 3.639
货号: ZQ0057
产品名称: HT29 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/jsfa.11487
论文标题: Sulforaphane suppresses carcinogenesis of colorectal cancer through the ERK/Nrf2‑UDP glucuronosyltransferase 1A metabolic axis activation
DOI: 10.3892/or.2020.7495
发表时间: 2020-02-10
期刊: ONCOLOGY REPORTS
影响因子: 3.417
货号: ZQ0057
产品名称: HT-29 cells
原文链接: https://www.spandidos-publications.com/10.3892/or.2020.7495
论文标题: LINC01198 promotes colorectal cancer cell proliferation and inhibits apoptosis via Notch signaling pathway.
DOI: 10.26355/eurrev_202008_22641
发表时间: 2020-08-01
期刊: European Review for Medical and Pharmacological Sciences
影响因子: 3.024
货号: ZQ0057
产品名称: HT-29 cells
原文链接: https://europepmc.org/article/med/32894550?javascript_support=no
论文标题: Preparing Sodium Alginate/Polyethyleneimine Spheres for Potential Application of Killing Tumor Cells by Reducing the Concentration of Copper Ions in the Lesions of Colon Cancer
DOI: 10.3390/ma12091570
发表时间: 2019-05-13
期刊: Materials
影响因子: 2.972
货号: ZQ0057
产品名称: HT-29 cells
原文链接: https://www.mdpi.com/461434
论文标题: Engineering NK-92 Cell by Upregulating CXCR2 and IL-2 Via CRISPR-Cas9 Improves Its Antitumor Effects as Cellular Immunotherapy for Human Colon Cancer
DOI: 10.1089/jir.2021.0078
发表时间: 2021-12-15
期刊: JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
影响因子: 2.607
货号: ZQ0057
产品名称: HT29 cells
原文链接: https://www.liebertpub.com/doi/abs/10.1089/jir.2021.0078
论文标题: Down-regulation of lncRNA DNAJC3-AS1 inhibits colon cancer via regulating miR-214-3p/LIVIN axis
DOI: 10.1080/21655979.2020.1757224
发表时间: 2020-04-30
期刊: Bioengineered
影响因子: 2.205
货号: ZQ0057
产品名称: HT29 cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/21655979.2020.1757224
货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0058 | LOVO人结肠癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1400.00 | 放入购物车 》 |
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ZQ0124 | COLO205人结肠癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1500.00 | 放入购物车 》 |
ZQ0125 | HCT-116人结肠癌细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ0190 | CT26.WT小鼠结肠癌细胞(种属鉴定) | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ0329 | RKO-AS45-1人结肠癌转基因细胞(暂不提供) | 5 x 10^5 cells/vial | ¥1550.00 | 放入购物车 》 |
ZQ0330 | RKO-E6人结肠癌转基因细胞(STR鉴定) | 5 x 10^5 cells/vial | ¥1500.00 | 放入购物车 》 |
ZQ506 | 支原体清除试剂盒 | 1ml | ¥600.00 | 放入购物车 》 |
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5 | 荧光素酶标记 | ¥询价 | 放入购物车 》 | |
7 | 细胞基因敲除 | ¥询价 | 放入购物车 》 | |
ZQ-100 | DMEM(高糖)基础培养基 | 500ml | ¥56.00 | 放入购物车 》 |
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ZQ500-A | 优级胎牛血清 | 500ml | ¥2580(开学促销) | 放入购物车 》 |