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SW480人结肠癌细胞(STR鉴定)

英文名:SW480
货号:ZQ0063
价格:¥1350.00
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推荐组合

SW480人结肠癌细胞(STR鉴定)

¥1350.00
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SW480 [SW-480]人结肠腺癌细胞专用培养基

¥350.00 ¥550.00

配套完培,组合使用,省时省力!

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细胞套餐惊爆价

¥1700 ¥2150.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

SW480人结肠癌细胞

货号

ZQ0063

产品介绍

SW480是一种人结肠腺癌细胞系,SW480和SW620均源自同一位男性患者,其中SW480是原发性结肠腺癌。最初是从一位50岁白人男性患者的直肠腺癌组织中分离得到的。这个细胞系在癌症研究中非常有用,尤其是在研究结直肠癌的生物学行为和药物反应方面。该细胞的CSAp和结肠抗原3阴性,角蛋白免疫过氧化物酶染色阳性。该细胞的p53基因第273位密码子的G->A突变导致Arg->His替代,第309位密码子的C->T突变导致Pro->Ser替代。该细胞p53蛋白表达水平升高,癌基因c-myc、K-ras、H-ras、N-ras、myb、sis和fos的表达呈阳性,但未检测到癌基因N-myc的表达。此外,该细胞不表达细胞基质裂解酶Matrilysin(一种与肿瘤入侵相关的金属蛋白酶)。有报道称该细胞表达GM-CSF。此外,该细胞K-RAS蛋白第12位氨基酸Gly突变为Val(c.35G>T),常被用于PCR检测该位点突变的阳性对照。该细胞是研究结直肠癌的重要工具,为科学家们提供了一个模型系统,用于研究疾病的进展和开发新的治疗方法。

注意事项:
1.该细胞使用的L-15基础培养液由磷酸盐和游离碱氨基酸代替了碳酸氢钠缓冲体系。该培养基必须在没有二氧化碳平衡的环境中培养细胞,不能在普通的5%二氧化碳细胞培养箱中培养,否则培养液颜色会明显变黄。

种属

性别/年龄

/50岁

组织

结肠

疾病

杜克斯氏B型,结肠直肠腺癌

细胞类型

肿瘤细胞 

形态学

上皮

生长方式

贴壁

倍增时间

大约24~50小时 

培养基和添加剂(默认)

L-15基础(中乔新舟 货号:ZQ-1100+10%胎牛血清(中乔新舟  货号ZQ500-A+1%P/S(中乔新舟 货号:CSP006

注意默认方案培养条件:100%空气;37℃

培养方案B(可选)

DMEM (中乔新舟  货号:ZQ-100)+10%FBS(中乔新舟 货号:ZQ0500+1%双抗(中乔新舟   货号:CSP006

注意B方案培养条件:95%空气,5%CO2;37℃

推荐默认完全培养基货号

ZM0063

B方案完全培养基货号

ZM0063-B

生物安全等级

BSL-1

默认方案培养条件

100%空气;37℃

STR位点信息

Amelogenin: X
CSF1PO: 13,14
D13S317: 12
D16S539: 13
D5S818: 13
D7S820: 8
TH01: 8
TPOX: 11
vWA: 16
Penta_D: 9,15
Penta_E: 10
D3S1358: 15
D21S11: 30,30.2
D8S1179: 13
FGA: 24
D19S433: 13
D2S1338: 17,24
D18S51: 13

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC;DSMZ CCL-228;ECACC ACC-313;87092801

供应限制

仅供科研使用

货号

ZQ0063

发货规格

活细胞:T25培养瓶*1瓶或者1毫升冻存管*1支(细胞量约为5个10^5细胞/瓶)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


论文标题microRNA-96通过调节AMPKα2-FTO-m6A/MYC轴促进结直肠癌的发生和发展
DOI: 10.1186/s13046-020-01731-7
发表时间: 2020-11-12
期刊:实验和临床癌症研究杂志
影响因子: 7.068
货号:ZQ0063
产品名称:SW480细胞
原文链接:https://link . springer . com/article/10.1186/s 13046-020-01731-7


论文标题: Knockdown of SFRS9 Inhibits Progression of Colorectal Cancer Through Triggering Ferroptosis Mediated by GPX4 Reduction
DOI: 10.3389/fonc.2021.683589
发表时间: 2021-07-16
期刊: Frontiers in Oncology
影响因子: 6.244
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8322952/


论文标题: Integrating network pharmacology deciphers the action mechanism of Zuojin capsule in suppressing colorectal cancer
DOI: 10.1016/j.phymed.2021.153881
发表时间: 2021-12-09
期刊: PHYTOMEDICINE
影响因子: 5.34
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0944711321004219


论文标题: Kinase GSK3β functions as a suppressor in colorectal carcinoma through the FTO-mediated MZF1/c-Myc axis
DOI: 10.1111/jcmm.16291
发表时间: 2021-02-02
期刊: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
影响因子: 5.31
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16291


论文标题: The function of Piezo1 in colon cancer metastasis and its potential regulatory mechanism
DOI: 10.1007/s00432-020-03179-w
发表时间: 2020-03-09
期刊: JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
影响因子: 3.656
货号: ZQ0063
产品名称: SW-480 cells
原文链接: https://link.springer.com/article/10.1007/s00432-020-03179-w 论文标题: MiR-346-5p promotes colorectal cancer cell proliferation in vitro and in vivo by targeting FBXL2 and activating the β-catenin signaling pathway
DOI: 10.1016/j.lfs.2020.117300
发表时间: 2020-01-14
期刊: LIFE SCIENCES
影响因子: 3.647
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0024320520300473


论文标题: Sulforaphane suppresses carcinogenesis of colorectal cancer through the ERK/Nrf2‑UDP glucuronosyltransferase 1A metabolic axis activation
DOI: 10.3892/or.2020.7495
发表时间: 2020-02-10
期刊: ONCOLOGY REPORTS
影响因子: 3.417
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.spandidos-publications.com/10.3892/or.2020.7495


论文标题: Brusatol Inhibits Proliferation and Metastasis of Colorectal Cancer by Targeting and Reversing the RhoA/ROCK1 Pathway
DOI: 10.1155/2022/7132159
发表时间: 2022-05-18
期刊: Biomed Research International
影响因子: 3.411
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.hindawi.com/journals/bmri/2022/7132159/


论文标题: LINC01198 promotes colorectal cancer cell proliferation and inhibits apoptosis via Notch signaling pathway.
DOI: 10.26355/eurrev_202008_22641
发表时间: 2020-08-01
期刊: European Review for Medical and Pharmacological Sciences
影响因子: 3.024
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://europepmc.org/article/med/32894550?javascript_support=no


论文标题: miR-196a-5p promotes metastasis of colorectal cancer via targeting IκBα
DOI: 10.1186/s12885-018-5245-1
发表时间: 2019-01-08
期刊: BMC CANCER
影响因子: 2.933
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://link.springer.com/article/10.1186/s12885-018-5245-1


论文标题: Evodiamine inhibits vasculogenic mimicry in HCT116 cells by suppressing hypoxia-inducible factor 1-alpha-mediated angiogenesis
DOI: 10.1097/CAD.0000000000001030
发表时间: 2021-01-08
期刊: ANTI-CANCER DRUGS
影响因子: 2.248
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7861498/


论文标题: Piperine inhibits colorectal cancer migration and invasion by regulating STAT3/Snail-mediated epithelial–mesenchymal transition
DOI: 10.1007/s10529-020-02923-z
发表时间: 2020-06-04
期刊: BIOTECHNOLOGY LETTERS
影响因子: 1.977
货号: ZQ0063
产品名称: SW480 cells
原文链接: https://link.springer.com/content/pdf/10.1007/s10529-020-02923-z.pdf


论文标题: Two new compounds from Carpesium abrotanoides
DOI: 10.1016/j.phytol.2020.08.012
发表时间: 2020-09-17
期刊: Phytochemistry Letters
影响因子: 1.459
货号: ZQ0063
产品名称: SW480 cells

原文链接: https://www.sciencedirect.com/science/article/pii/S187439002030639X



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