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Thp-1人单核细胞白血病(STR鉴定)

英文名:Thp-1
货号:ZQ0086
价格:¥1600.00
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推荐组合

Thp-1人单核细胞白血病(STR鉴定)

¥1600.00
+

THP-1人单核细胞白血病专用培养基

¥550.00

配套完培,组合使用,省时省力!

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细胞套餐惊爆价

¥2150
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

Thp-1人单核细胞白血病

货号

ZQ0086

产品介绍

THP-1是一种人急性单核细胞白血病细胞系,最初从一位1岁患有急性单核细胞白血病的男孩的外周血中分离出来的单核细胞。该细胞对乳汁珠和激活的红细胞有吞噬作用,无表面和胞质免疫球蛋白。 可以用佛波醇 TPA诱导单核细胞分化。表达C3R和FcR;可作为转染宿主。也因其能够被诱导分化成单核/巨噬细胞,所以在免疫学研究中具有广泛的应用。例如:巨噬细胞分化与炎症模型、基因编辑研究、免疫调节作用研究、疾病模型构建与药物筛选、STING激动剂筛选模型、细胞凋亡和细胞周期研究、表观遗传调控研究等。这些应用展示了THP-1细胞系在免疫学研究中的多样性和重要性,为理解免疫调节和疾病机制提供了宝贵的工具。

【注意事项】:
1.该细胞为悬浮细胞。根据我司培养经验,建议传代时使用【半换液法】对细胞状态有利。当培养基颜色比较黄建议离心换液,培养基颜色变化不明显且细胞数量多可以进行补液,然后将细胞吹打用手指弹松后均匀移入两个新的 T25培养瓶中维续培养即可(1:2 传代)。
2. 该细胞在培养过程中,可能会有少量贴壁或聚集成小团生长,属于正常情况。传代时可以轻轻打散,如少许细胞已贴壁则传代时丢弃,小团生长的细胞正常传代培养即可。
3. 该细胞对细胞密度较为敏感,培养、传代时请注意保持细胞密度在合适的范围。
4.  该细胞较难复苏,复苏后需要约7天左右才可能恢复至正常状态。刚复苏后,细胞生长缓慢且会出现黑色细胞碎片,少量细胞碎片不影响细胞正常生长,待细胞恢复生长后用半换液法可以稀释碎片。
5. 细胞冻存时务请提高细胞密度,建议1个T25培养瓶中含有3-5×106细胞量时冻存一支。
6. 该细胞对血清质量较为敏感,我司建议您使用优质胎牛血清进行培养或选择订购我司配套THP-1完全培养液。

种属

性别/年龄

男/1岁

组织

外周血

疾病

急性单核细胞白血病

细胞类型

肿瘤细胞

形态学

单核细胞

生长方式

悬浮

倍增时间

大约26~70小时

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500+1%双抗(中乔新舟   货号:CSP006+β-Mer 终浓度:0.05mM(中乔新舟  货号:CSP005,例:500ml添加500ul

推荐完全培养基货号

ZM0086

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y

CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
TH01: 8,9.3
TPOX: 8,11
vWA: 16
D3S1358: 15,17
D21S11: 30,31.2
D18S51: 13,14
D8S1179: 10,14
FGA: 24,25
D19S433: 12.2,13
D2S1338: 17,18

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

*** 

基因表达

*** 

保藏机构

ATCC; TIB-202 DSMZ; ACC-16 ECACC; 88081201

供应限制

仅供科研使用


货号

ZQ0086

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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发表时间: 2021-11-05
期刊: BRIEFINGS IN BIOINFORMATICS
影响因子: 11.622
货号: ZQ0086
产品名称: THP-1 cells
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DOI: 10.1016/j.actbio.2022.04.010
发表时间: 2022-04-12
期刊: Acta Biomaterialia
影响因子: 8.947
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S1742706122002148


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DOI: 10.1093/rb/rbab056
发表时间: 2021-09-02
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货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://academic.oup.com/rb/article-abstract/8/6/rbab056/6408453


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发表时间: 2021-02-01
期刊: BIOCHEMICAL PHARMACOLOGY
影响因子: 5.858
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0006295221000320


论文标题: The new mitochondrial uncoupler BAM15 induces ROS production for treatment of acute myeloid leukemia
DOI: 10.1016/j.bcp.2022.114948
发表时间: 2022-02-19
期刊: BIOCHEMICAL PHARMACOLOGY
影响因子: 5.858
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0006295222000429

论文标题: A novel role of VEPH1 in regulating AoSMC phenotypic switching
DOI: 10.1002/jcp.29736
发表时间: 2020-04-27
期刊: JOURNAL OF CELLULAR PHYSIOLOGY
影响因子: 5.546
货号: ZQ0086
产品名称: THP1 cells

原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcp.29736


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DOI: 10.3389/fphys.2021.644903
发表时间: 2021-07-09
期刊: Frontiers in Physiology
影响因子: 4.566
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8299470/


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发表时间: 2019-05-24
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发表时间: 2020-04-09
期刊: JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY
影响因子: 4.133
货号: ZQ0086
产品名称: THP-1 monocytes
原文链接: https://www.sciencedirect.com/science/article/pii/S0022282820300900


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发表时间: 2021-09-03
期刊: INFLAMMATION
影响因子: 4.092
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://link.springer.com/article/10.1007/s10753-021-01551-7


论文标题: mTORC1 signaling pathway regulates macrophages in choroidal neovascularization
DOI: 10.1016/j.molimm.2020.03.002
发表时间: 2020-03-12
期刊: MOLECULAR IMMUNOLOGY
影响因子: 3.641
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0161589019308636


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DOI: 10.1016/j.bbrc.2021.05.011
发表时间: 2021-05-15
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子: 3.575
货号: ZQ0086
产品名称: THP-1 monocytes
原文链接: https://www.sciencedirect.com/science/article/pii/S0006291X21007816


论文标题: MicroRNA-216a Promotes Endothelial Inflammation by Smad7/IκBα Pathway in Atherosclerosis
DOI: 10.1155/2020/8864322
发表时间: 2020-11-18
期刊: DISEASE MARKERS
影响因子: 2.738
货号: ZQ0086
产品名称: THP-1 monocytes
原文链接: https://www.hindawi.com/journals/dm/2020/8864322/


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DOI: 10.1155/2022/5954963
发表时间: 2022-06-06
期刊: Evidence-based Complementary and Alternative Medicine
影响因子: 2.65
货号: ZQ0086
产品名称: THP-1 cells
原文链接: https://www.hindawi.com/journals/ecam/2022/5954963/


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发表时间: 2020-04-06
期刊: IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
影响因子: 2.352
货号: ZQ0086
产品名称: THP-1 cells
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DOI: 10.1111/ijlh.13305
发表时间: 2020-10-02
期刊: International Journal of Laboratory Hematology
影响因子: 2.141
货号: ZQ0086
产品名称: THP-1 cells
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DOI: 10.3892/mmr.2020.10987
发表时间: 2020-04-01
期刊: Molecular Medicine Reports
影响因子: 2.1
货号: ZQ0086
产品名称: THP-1 cells
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DOI: 10.3892/etm.2020.9507
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期刊: Experimental and Therapeutic Medicine
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DOI: 10.1177/1721727X221110348
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期刊: European Journal of Inflammation
影响因子: 0.424
货号: ZQ0086
产品名称: THP-1 monocytes

原文链接: https://journals.sagepub.com/doi/abs/10.1177/1721727X221110348



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Development of an in vitro skin sensitization test using human cell lines; human Cell Line Activation Test (h-CLAT). II. An inter-laboratory study of the h-CLAT.
Toxicol. In Vitro 20:774-784(2006)


PubMed=16408098; DOI=10.1038/sj.leu.2404081
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JAK2 V617F tyrosine kinase mutation in cell lines derived from myeloproliferative disorders.
Leukemia 20:471-476(2006)


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Riken Omics Science Center
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Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
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THP-1 macrophages and SGBS adipocytes -- a new human in vitro model system of inflamed adipose tissue.
Front. Endocrinol. 2:89.1-89.8(2011)


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The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
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Chauhan V., Howland M., Wilkins R.C.
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Chauhan V., Howland M.
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