产品名称
U-2OS(U2OS)人骨肉瘤细胞
货号
ZQ0121
产品介绍
U-2OS(U2OS)是一种人骨肉瘤细胞系,最初由Ponten J和Saksela E在1964年从一名15岁女孩的胫骨中等分化的肉瘤中分离建立。这种细胞系具有上皮样的形态特征,并且是贴壁生长的细胞。
该细胞对于癌症研究,尤其是骨癌研究具有重要价值。这些细胞在癌症生物学、药物筛选、细胞凋亡、遗传学以及放射肿瘤学的研究中被广泛应用。U2OS细胞的应用价值体现在它们对于研究细胞凋亡和治疗耐药性的重要性,这对于开发小分子抑制剂和其他治疗药物至关重要。
种属
人
性别/年龄
女/15岁
组织
骨;骨肉瘤
疾病
骨肉瘤(NCIt:C9145)
生物安全等级
BSL-1
STR位点信息
Amelogenin: X
D3S1358: 16
细胞类型
肿瘤细胞
形态学
上皮细胞
生长方式
贴壁生长
倍增时间
大约25~36小时
培养基和添加剂
90%DMEM高糖(品牌:中乔新舟 货号:ZQ-100)+10%FBS(品牌:中乔新舟 货号:ZQ0500)+1%PS(中乔新舟 货号:CSP006)
推荐完全培养基货号
培养条件
37℃
抗原表达/受体表达
***
基因表达
***
保藏机构
ATCC、ECACC、DSMZ、
供应限制
仅供科研使用
在骨肉瘤的临床研究中,U2OS细胞系有助于探究放疗的生物学效应,增进了我们对骨肉瘤生物学特性的认识。此外,这些细胞也是研究染色质修饰及其对细胞生物学功能影响的重要工具,尤其是在肿瘤形成和癌症发展的过程中。
已被证实该细胞具有在体内形成肿瘤的能力,无论是通过皮下还是肌肉注射的方式。由U2OS细胞形成的肿瘤被分类为高级别肉瘤,并具有明显的骨样组织形成,这是骨肉瘤的特征之一。此外,这些肿瘤中还观察到免疫细胞的浸润,使得U2OS细胞成为研究人类骨肉瘤及其与人体免疫系统相互作用、肿瘤免疫学的重要模型。然而,考虑到肿瘤形成的变异性,确保U2OS骨肉瘤细胞系能够准确模拟体内肿瘤的挑战依然存在。
总之,骨肉瘤细胞系,如U2OS,是理解骨肉瘤的关键工具,为癌症生物学、治疗策略的发展以及肿瘤与免疫系统相互作用的复杂性提供了深刻的见解,同时也凸显了建立精确的体内肿瘤模型的重要性。
TH01: 6,9.3
D21S11: 31
D18S51: 14
Penta_E: 10,13
D5S818: 11
D13S317: 13
D7S820: 11,12
D16S539: 11,12
CSF1PO: 13
Penta_D: 9
vWA: 14,18
D8S1179: 12,14
TPOX: 11,12
FGA: 20
D19S433: 15
D2S1338: 20,24
|
1、论文标题: Combining autophagy-inducing peptides and brefeldin A delivered by perinuclear-localized mesoporous silica nanoparticles: a manipulation strategy for ER-phagy
DOI: 10.1039/C8NR00872H
发表时间: 2018-04-11
期刊: Nanoscale
影响因子: 7.233
货号: ZQ0121
产品名称: U2OS cells
原文链接: https://pubs.rsc.org/en/content/articlehtml/2018/nr/c8nr00872h
2、论文标题: Activation of CXCL6/CXCR1/2 Axis Promotes the Growth and Metastasis of Osteosarcoma Cells in vitro and in vivo
DOI: 10.3389/fphar.2019.00307
发表时间: 2019-03-28
期刊: Frontiers in Pharmacology
影响因子: 5.811
货号: ZQ0121
产品名称: U2OS cell lines
原文链接: https://www.frontiersin.org/articles/10.3389/fphar.2019.00307/full
3、论文标题: ADAM10 promotes cell growth, migration, and invasion in osteosarcoma via regulating E-cadherin/β-catenin signaling pathway and is regulated by miR-122-5p
DOI: 10.1186/s12935-020-01174-2
发表时间: 2020-03-30
期刊: Cancer Cell International
影响因子: 5.722
货号: ZQ0121
产品名称: U-2OS cells
原文链接: https://cancerci.biomedcentral.com/articles/10.1186/s12935-020-01174-2
4、论文标题: NPR3, transcriptionally regulated by POU2F1, inhibits osteosarcoma cell growth through blocking the PI3K/AKT pathway
DOI: 10.1016/j.cellsig.2021.110074
发表时间: 2021-07-03
期刊: CELLULAR SIGNALLING
影响因子: 4.315
货号: ZQ0121
产品名称: U2OS cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0898656821001637
5、论文标题: Mxd1 mediates hypoxia‐induced cisplatin resistance in osteosarcoma cells by repression of the PTEN tumor suppressor gene
DOI: 10.1002/mc.22676
发表时间: 2017-06-15
期刊: MOLECULAR CARCINOGENESIS
影响因子: 4.185
货号: ZQ0121
产品名称: U-2OS cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/mc.22676
6、论文标题: Antitumor Effect of miR-1294/Pyruvate Kinase M2 Signaling Cascade in Osteosarcoma Cells
DOI: 10.2147/OTT.S232718
发表时间: 2020-02-21
期刊: OncoTargets and Therapy
影响因子: 4.147
货号: ZQ0121
产品名称: U2OS cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7041606/
7、论文标题: Salidroside suppresses the growth and invasion of human osteosarcoma cell lines MG63 and U2OS in vitro by inhibiting the JAK2/STAT3 signaling pathway
DOI: 10.3892/ijo.2019.4781
发表时间: 2019-06-01
期刊: INTERNATIONAL JOURNAL OF ONCOLOGY
影响因子: 3.571
货号: ZQ0121
产品名称: U2OS cells
原文链接: https://www.spandidos-publications.com/10.3892/ijo.2019.4781?text=fulltext
8、论文标题: miR-328-3p mediates the anti-tumor effect in osteosarcoma via directly targeting MMP-16
DOI: 10.1186/s12935-019-0829-7
发表时间: 2019-04-23
期刊: Cancer Cell International
影响因子: 3.439
货号: ZQ0121
产品名称: U2OS cells
原文链接: https://cancerci.biomedcentral.com/articles/10.1186/s12935-019-0829-7
9、论文标题: Circular RNA circVRK1 suppresses the proliferation, migration and invasion of osteosarcoma cells by regulating zinc finger protein ZNF652 expression via microRNA miR-337-3p
DOI: 10.1080/21655979.2021.1965695
发表时间: 2021-08-23
期刊: Bioengineered
影响因子: 3.269
货号: ZQ0121
产品名称: U-2OS cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.1965695
10、论文标题: Piperlongumine induces apoptosis and G2/M phase arrest in human osteosarcoma cells by regulating ROS/PI3K/Akt pathway
DOI: 10.1016/j.tiv.2020.104775
发表时间: 2020-01-24
期刊: TOXICOLOGY IN VITRO
影响因子: 2.959
货号: ZQ0121
产品名称: U2OS cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0887233319307799
11、论文标题: P16 promotes the growth and mobility potential of breast cancer both in vitro and in vivo: the key role of the activation of IL-6/JAK2/STAT3 signaling
DOI: 10.1007/s11010-018-3281-4
发表时间: 2018-01-31
期刊: MOLECULAR AND CELLULAR BIOCHEMISTRY
影响因子: 2.561
货号: ZQ0121
产品名称: U-2OS cells
原文链接: https://link.springer.com/article/10.1007/s11010-018-3281-4
12、论文标题: Effect of lncRNA ANRIL knockdown on proliferation and cisplatin chemoresistance of osteosarcoma cells in vitro
DOI: 10.1016/j.prp.2019.01.042
发表时间: 2019-01-30
期刊: PATHOLOGY RESEARCH AND PRACTICE
影响因子: 1.794
货号: ZQ0121
产品名称: U2-OS cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0344033818316558
13、论文标题: MicroRNA‑208a‑3p promotes osteosarcoma progression via targeting PTEN
DOI: 10.3892/etm.2020.9385
发表时间: 2020-10-23
期刊: Experimental and Therapeutic Medicine
影响因子: 1.785
货号: ZQ0121
产品名称: U2OS cells
原文链接: https://www.spandidos-publications.com/10.3892/etm.2020.9385
14、论文标题: Knockdown of microRNA-203 reduces cisplatin chemo-sensitivity to osteosarcoma cell lines MG63 and U2OS in vitro by targeting RUNX2
DOI: 10.1080/1120009X.2021.1899441
发表时间: 2021-03-25
期刊: JOURNAL OF CHEMOTHERAPY
影响因子: 1.714
货号: ZQ0121
产品名称: U2OS cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/1120009X.2021.1899441
15、论文标题: MicroRNA-506-3p initiates mesenchymal-to-epithelial transition and suppresses autophagy in osteosarcoma cells by directly targeting SPHK1
DOI: 10.1080/09168451.2019.1569496
发表时间: 2019-01-23
期刊: BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
影响因子: 1.297
货号: ZQ0121
产品名称: U-2OS cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/09168451.2019.1569496
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Detection of viral proteins in human cells lines by xeno-proteomics: elimination of the last valid excuse for not testing every cellular proteome dataset for viral proteins.
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21、PubMed=25877200; DOI=10.1038/nature14397
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Nature 520:307-311(2015)
22、PubMed=25894527; DOI=10.1371/journal.pone.0121314
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PLoS ONE 10:E0121314-E0121314(2015)
23、PubMed=26351324; DOI=10.1158/1535-7163.MCT-15-0074
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货号 | 产品名称 | 规格 | 价格 | 指令 |
ZQ0403 | MG-63人骨肉瘤细胞 | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ0405 | MNNG/HOS Cl #5 [R-1059-D]人骨肉瘤细胞 | 5 x 10^5 cells/vial | ¥1600.00 | 放入购物车 》 |
ZQ0455 | 143B人骨肉瘤细胞(STR鉴定)[套餐促销] | 5 x 10^5 cells/vial | ¥1400.00 | 放入购物车 》 |
ZQ506 | 支原体清除试剂盒 | 1ml | ¥600.00 | 放入购物车 》 |
A1001-1 | 国产转染试剂 | 1ml | ¥800.00 | 放入购物车 》 |
1 | 慢病毒介导基因沉默或过表达 | ¥询价 | 放入购物车 》 | |
5 | 荧光素酶标记 | ¥询价 | 放入购物车 》 | |
ZQ-100 | DMEM(高糖)基础培养基 | 500ml | ¥56.00 | 放入购物车 》 |
ZQ-101 | DMEM(高糖)完全培养基 | 500ML | ¥350.00 | 放入购物车 》 |
ZQ500-A | 优级胎牛血清 | 500ml | ¥2580(开学促销) | 放入购物车 》 |
JSFW-134 | 人源细胞STR鉴定服务 | ¥900.00 | 放入购物车 》 |