产品名称	HGC-27人胃癌细胞（未分化）
货号	ZQ0192
产品介绍	HGC-27是一种人胃癌细胞系，源自一名成年胃癌患者的淋巴结转移灶，这种细胞系由未分化胃癌细胞组成，能够产生并分泌黏液素。该细胞系表现出上皮形态。 HGC-27细胞已在众多研究中被用来调查癌细胞增殖、凋亡和转移的机制。它们作为理解胃癌中涉及的复杂分子相互作用和途径的宝贵模型，包括对治疗化合物的反应和新药物靶点的研究。这些细胞在研究胃癌进展中各种遗传和表观遗传修饰的作用方面也很重要。使用HGC-27的研究表明了细胞过程如上皮-间充质转化（EMT）的见解，这是癌症转移中的关键事件。此外，该细胞系已被用来探索受体信号通路及其对癌细胞行为的影响，为靶向治疗的发展提供了关键数据。 HGC-27细胞系在胃癌研究中的应用有以下案例：黄芩素对胃癌HGC-27细胞上皮间充质转化进程的影响研究、紫杉醇耐药人胃癌细胞HGC-27/PTX的建立及其特征鉴定、虫草素对人胃癌细胞系HGC-27的影响及分子机制、过表达FAF1对胃癌细胞HGC-27增殖及凋亡的影响、SOX9在胃癌组织中的表达及其对胃癌细胞HGC-27生物学功能的影响、G蛋白偶联受体34敲低损害HGC-27胃癌细胞的体外增殖和迁移、人胃癌HGC-27细胞及其肿瘤球细胞裸小鼠皮下移植的研究等。 总的来说，HGC-27是胃癌研究进展中的重要工具，有助于为新的治疗策略铺平道路，提高我们对疾病机制的理解。
种属	人
性别/年龄	***/成年人
组织	胃，淋巴结转移
疾病	胃癌
细胞类型	肿瘤细胞
形态学	上皮细胞
生长方式	贴壁
倍增时间	大约17小时
培养基和添加剂	RPMI-1640（中乔新舟 货号：ZQ-200）+20%胎牛血清（中乔新舟 货号：ZQ0500）+1%双抗（中乔新舟   货号：CSP006）
推荐完全培养基货号	ZM0192
生物安全等级	BSL-1
STR位点信息	Amelogenin: X D5S818: 12 D13S317: 10,11 D7S820: 11,12,13 D16S539: 10,11 vWA: 14 TH01: 9 TPOX: 8 CSF1PO: 12 D19S433: 14 D21S11: 30,33,34 D18S51: 16,17 D6S1043: 18 D3S1358: 17 Penta D: 9,13 D2S441: 9.1,13 D8S1179: 11,16 Penta E: 18 D12S391: 20,21 D2S1338: 22,24 FGA: 22
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达	***
基因表达	***
保藏机构	BCRJ; 0310 ；ECACC; 94042256 ；ICLC; HTL95028 ；KCB; KCB 201601YJ ；RCB; RCB0500
供应限制	仅供科研使用

货号	ZM0192
发货规格	活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一
发货形式	活细胞：常温运输；冻存管：干冰运输
储存温度	活细胞：培养箱；冻存管：液氮罐
产地	中国
供应限制	仅供科研使用

论文标题： ELK4 promotes the development of gastric cancer by inducing M2 polarization of macrophages through regulation of the KDM5A-PJA2-KSR1 axis
DOI： 10.1186/s12967-021-02915-1
发表时间： 2021-08-09
期刊： Journal of Translational Medicine
影响因子： 5.531
货号： ZQ0192
产品名称： HGC-27 cells

原文链接： https://link.springer.com/article/10.1186/s12967-021-02915-1

论文标题： LINC_00355 promotes gastric cancer progression by upregulating PHF19 expression through sponging miR-15a-5p
DOI： 10.1186/s12885-021-08227-3
发表时间： 2021-06-02
期刊： BMC CANCER
影响因子： 4.43
货号： ZQ0192
产品名称： HGC-27 cells
原文链接： https://bmccancer.biomedcentral.com/articles/10.1186/s12885-021-08227-3

论文标题： Targeting ADAR1 suppresses progression and peritoneal metastasis of gastric cancer through Wnt / β-catenin pathway
DOI： 10.7150/jca.61031
发表时间： 2021-10-28
期刊： Journal of Cancer
影响因子： 4.207
货号： ZQ0192
产品名称： HGC-27 cells
原文链接： https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8734406/

论文标题： Knockdown of ROS proto-oncogene 1 inhibits migration and invasion in gastric cancer cells by targeting the PI3K/Akt signaling pathway
DOI： 10.2147/OTT.S213421
发表时间： 2019-10-16
期刊： OncoTargets and Therapy
影响因子： 4.147
货号： ZQ0192
产品名称： HGC-27 cells
原文链接： https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6801563/

论文标题： Silencing Formin-like 2 inhibits growth and metastasis of gastric cancer cells through suppressing internalization of integrins
DOI： 10.1186/s12935-018-0576-1
发表时间： 2018-06-01
期刊： Cancer Cell International
影响因子： 3.96
货号： ZQ0192
产品名称： HGC-27 cells
原文链接： https://cancerci.biomedcentral.com/articles/10.1186/s12935-018-0576-1

论文标题： Clinical significance and biological function of transcriptional repressor GATA binding 1 in gastric cancer: a study based on data mining, RT-qPCR, immunochemistry, and vitro experiment
DOI： 10.1080/15384101.2020.1827499
发表时间： 2020-10-12
期刊： CELL CYCLE
影响因子： 3.699
货号： ZQ0192
产品名称： HGC-27 cells
原文链接： https://www.tandfonline.com/doi/abs/10.1080/15384101.2020.1827499

论文标题： The Regulatory Mechanism and Biological Significance of Mitochondrial Calcium Uniporter in the Migration, Invasion, Angiogenesis and Growth of Gastric Cancer
DOI： 10.2147/OTT.S262049
发表时间： 2020-11-17
期刊： OncoTargets and Therapy
影响因子： 3.337
货号： ZQ0192
产品名称： HGC-27 cells
原文链接： https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7680189/

论文标题： Dihydroartemisinin suppresses proliferation, migration, the Wnt/β‑catenin pathway and EMT via TNKS in gastric cancer Corrigendum in /10.3892/ol.2021.13152
DOI： 10.3892/ol.2021.12949
发表时间： 2021-07-29
期刊： Oncology Letters
影响因子： 2.967
货号： ZQ0192
产品名称： HGC-27 cells
原文链接： https://www.spandidos-publications.com/10.3892/ol.2021.12949

论文标题： Knockdown of lncRNA SNHG4 suppresses gastric cancer cell proliferation and metastasis by targeting miR-204-5p.
DOI： 10.4149/neo_2021_200914n981
发表时间： 2021-02-11
期刊： NEOPLASMA
影响因子： 2.575
货号： ZQ0192
产品名称： "human gastric
cancer cell lines HGC-27"
原文链接： https://europepmc.org/article/med/33567852?javascript_support=no

论文标题： miR‐375‐3p/YWHAZ/β‐catenin axis regulates migration, invasion, EMT in gastric cancer cells
DOI： 10.1111/1440-1681.13047
发表时间： 2018-12-21
期刊： CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
影响因子： 2.092
货号： ZQ0192
产品名称： HGC-27 cells

原文链接： https://onlinelibrary.wiley.com/doi/abs/10.1111/1440-1681.13047

PubMed=136873; DOI=10.18926/AMO/31422
Akagi T., Kimoto T.
Human cell line (HGC-27) derived from the metastatic lymph node of gastric cancer.
Acta Med. Okayama 30:215-219(1976)

PubMed=3986962; DOI=10.1093/carcin/6.4.549
Watatani M., Ikenaga M., Hatanaka T., Kinuta M., Takai S.-i., Mori T., Kondo S.
Analysis of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced DNA damage in tumor cell strains from Japanese patients and demonstration of MNNG hypersensitivity of Mer xenografts in athymic nude mice.
Carcinogenesis 6:549-553(1985)

PubMed=11314020; DOI=10.1038/sj.onc.1204160
Kataoka H., Miura Y., Joh T., Seno K., Tada T., Tamaoki T., Nakabayashi H., Kawaguchi M., Asai K., Kato T., Itoh M.
Alpha-fetoprotein producing gastric cancer lacks transcription factor ATBF1.
Oncogene 20:869-873(2001)

PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)

PubMed=15767549; DOI=10.1158/1535-7163.MCT-04-0234
Nakatsu N., Yoshida Y., Yamazaki K., Nakamura T., Dan S., Fukui Y., Yamori T.
Chemosensitivity profile of cancer cell lines and identification of genes determining chemosensitivity by an integrated bioinformatical approach using cDNA arrays.
Mol. Cancer Ther. 4:399-412(2005)

PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
Murai Y., Hayashi S., Takahashi H., Tsuneyama K., Takano Y.
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
Pathol. Res. Pract. 201:109-115(2005)

PubMed=20164919; DOI=10.1038/nature08768
Bignell G.R., Greenman C.D., Davies H., Butler A.P., Edkins S., Andrews J.M., Buck G., Chen L., Beare D., Latimer C., Widaa S., Hinton J., Fahey C., Fu B.-Y., Swamy S., Dalgliesh G.L., Teh B.T., Deloukas P., Yang F.-T., Campbell P.J., Futreal P.A., Stratton M.R.
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=20215515; DOI=10.1158/0008-5472.CAN-09-3458
Rothenberg S.M., Mohapatra G., Rivera M.N., Winokur D., Greninger P., Nitta M., Sadow P.M., Sooriyakumar G., Brannigan B.W., Ulman M.J., Perera R.M., Wang R., Tam A., Ma X.-J., Erlander M., Sgroi D.C., Rocco J.W., Lingen M.W., Cohen E.E.W., Louis D.N., Settleman J., Haber D.A.
A genome-wide screen for microdeletions reveals disruption of polarity complex genes in diverse human cancers.
Cancer Res. 70:2158-2164(2010)

PubMed=22460905; DOI=10.1038/nature11003
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)

PubMed=24807215; DOI=10.1038/ncomms4830
Liu J.-F., McCleland M.L., Stawiski E.W., Gnad F., Mayba O., Haverty P.M., Durinck S., Chen Y.-J., Klijn C., Jhunjhunwala S., Lawrence M., Liu H.-B., Wan Y.-N., Chopra V.S., Yaylaoglu M.B., Yuan W.-L., Ha C., Gilbert H.N., Reeder J., Pau G., Stinson J., Stern H.M., Manning G., Wu T.D., Neve R.M., de Sauvage F.J., Modrusan Z., Seshagiri S., Firestein R., Zhang Z.-M.
Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer.
Nat. Commun. 5:3830.1-3830.8(2014)

PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)

PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)

PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)

PubMed=27397505; DOI=10.1016/j.cell.2016.06.017
Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
Cell 166:740-754(2016)

PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)

PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)

PubMed=31068700; DOI=10.1038/s41586-019-1186-3
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023
Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E.T., Schweppe D.K., Jedrychowski M.P., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.
Quantitative proteomics of the Cancer Cell Line Encyclopedia.
Cell 180:387-402.e16(2020)

PubMed=35839778; DOI=10.1016/j.ccell.2022.06.010
Goncalves E., Poulos R.C., Cai Z.-X., Barthorpe S., Manda S.S., Lucas N., Beck A., Bucio-Noble D., Dausmann M., Hall C., Hecker M., Koh J., Lightfoot H., Mahboob S., Mali I., Morris J., Richardson L., Seneviratne A.J., Shepherd R., Sykes E., Thomas F., Valentini S., Williams S.G., Wu Y.-X., Xavier D., MacKenzie K.L., Hains P.G., Tully B., Robinson P.J., Zhong Q., Garnett M.J., Reddel R.R.
Pan-cancer proteomic map of 949 human cell lines.
Cancer Cell 40:835-849.e8(2022)