产品名称 |
ACHN人肾细胞腺癌细胞 |
货号 |
ZQ0340 |
产品介绍 |
ACHN细胞系来源于一位22岁白人男性肾腺癌广泛转移的恶性胸腔积液。该细胞系于1979年11月建立,随后将癌细胞直接播种到含有Eagle's MEM和10% FBS的培养瓶中。在150天的时间里,细胞被维持并在体外传代。随后,将这些细胞皮下接种到裸鼠体内,在四周内形成可触及的局部侵袭性肿瘤。该细胞系具有致瘤性,在接种107个细胞的裸鼠中100%(5/5)诱导肿瘤,21天内肿瘤发生。
【注意事项】该细胞生长受到人类干扰素抑制 |
种属 |
人 |
性别/年龄 |
男/22岁 |
组织 |
肾;来源于转移部位:胸腔积液 |
疾病 |
肾细胞腺癌 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮的 |
生长方式 |
贴壁 |
倍增时间 |
大约27.5~32小时 |
培养基和添加剂 |
MEM(含有NEAA)基础培养基(品牌:中乔新舟 货号:ZQ-300)+10%FBS(品牌:中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL-1 |
STR位点信息 |
Amelogenin: X CSF1PO: 11 D13S317: 12 D16S539: 12,13 D5S818: 12 D7S820: 9,11 TH01: 8 TPOX: 8,11 vWA: 16,17 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
ATCC; CRL-1611 ECACC; 88100508 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0340 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
论文标题: Triple-serotype chimeric oncolytic adenovirus exerts multiple synergistic mechanisms against solid tumors
DOI: 10.1136/jitc-2022-004691
发表时间: 2022-05-01
期刊: Journal for ImmunoTherapy of Cancer
影响因子: 13.751
货号: ZQ0340
产品名称: ACHN cells
原文链接: https://jitc.bmj.com/content/10/5/e004691.abstract
论文标题: Epigenetic inactivation of hydroxymethylglutaryl CoA synthase reduces ketogenesis and facilitates tumor cell motility in clear cell renal carcinoma
DOI: 10.1016/j.prp.2021.153622
发表时间: 2021-09-20
期刊: PATHOLOGY RESEARCH AND PRACTICE
影响因子: 3.25
货号: ZQ0340
产品名称: ccRCC cell lines ACHN
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