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MDA-MB-435S人黑素瘤细胞

英文名:MDA-MB-435S
货号:ZQ0374
价格:¥1350.00
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推荐组合

MDA-MB-435S人黑素瘤细胞

¥1350.00
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MDA-MB-435S人黑素瘤细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

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细胞套餐惊爆价

¥1700 ¥2030.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

MDA-MB-435S人黑素瘤细胞

货号

ZQ0374

产品介绍

该细胞系最初被描述为 R. Cailleau 等人于 1976 年分离的亲本 MDA-MB-435 菌株的纺锤形变体。来自一名患有转移性乳腺导管腺癌的 31 岁女性的胸腔积液。 

然而,最近的研究对亲本细胞系 MDA-MB-435 以及延伸的 HTB-129 的起源产生了疑问。对细胞的基因表达分析产生了微阵列,其中 MDA-MB-435 与黑色素瘤来源的细胞系聚集在一起。

此后的更多研究证实了 MDA-MB-435 的黑素细胞起源,ATCC 对此做出了回应,对该细胞系的身份进行了自己的调查。据报道,MDA-MB-435 交叉污染的细胞系是 M14 黑色素瘤细胞系 [PubMed ID:12354931 和 PubMed ID:17004106]。

种属

性别/年龄

/31岁

组织

先前描述为:乳腺/乳房;来源于转移部位:胸腔积液

疾病

无色素性黑色素瘤

细胞类型

黑色素细胞,黑色素瘤

形态学

纺锤形

生长方式

贴壁

倍增时间

大约48~72小时 

培养基和添加剂(默认)

DMEM (中乔新舟  货号:ZQ-100)+10%FBS(中乔新舟 货号:ZQ0500+1%双抗(中乔新舟   货号:CSP006

注意默认方案培养条件:95%空气,5%CO2;37℃

培养方案B(可选)

L-15(中乔新舟  货号:ZQ-1100+10%FBS(中乔新舟  货号:ZQ500-A+1%P/S(中乔新舟  货号:CSP006+0.01mg/mL牛胰岛素+0.01mg/ml谷胱甘肽

注意B方案培养条件:100%空气;37℃

推荐默认完全培养基货号

ZM0374

B方案完全培养基货号

ZM0374-B

生物安全等级

BSL-1

STR位点信息

Amelogenin: X

CSF1PO: 11

D13S317: 12

D16S539: 13

D5S818: 12

D7S820: 8,10

TH01: 6,7

TPOX: 8,11

vWA: 16,18

默认方案培养条件

95%空气,5%CO2;37℃

抗原表达/受体表达

***

基因表达

***

保藏机构

ATCC; HTB-129

供应限制

仅供科研使用

货号

ZQ0374

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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