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人丙型肝炎定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Hepatitis C qPCR Array Kit (GQH-HPC) is designed to facilitate gene expression profiling of 88 key genes involved in hepatitis C pathogenesis. Hepatitis C is a liver infectious disease caused by the Hepatitis C virus (HCV). The HCV exposure usually causes persistent liver infection and often develops into chronic liver disease. In response to hepatocyte HCV infection, antiviral immune response cascades are triggered and interferons are produced to modulate the immune system and suppress the spread of HCV. Brief examples of how included genes may be grouped are shown below:
HCV binding factor: LDLR, CD81, OCLN, SCARB1
Signaling pathways
RIG-1 like receptor: DDX58, MAVS, RAF3, TBK1, IKBKE, IRF3
Toll-like receptor: TLR3, TICAM1, RIPK1, IKBIB, IKBKG, CHUK
JAK/STAT: IFNAR1, IFNAR2, JAK1, TYK2, STAT1, STAT2, STAT6
Genes implicated in HCV infection: IFNA1, IFNA2, GPT, CLDN1, IFNB1, EIF2AK2, CLEC4M, RAF1, IRF3, HM13, GGT1
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Hepatitis C qPCR Array Plate Layout*(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AKT1 |
CLEC4M |
EIF3E |
IFNA2 |
IRF3 |
MAPK14 |
OAS1 |
PIK3R1 |
PPP2R1B |
RIPK1 |
STAT6 |
β-actin |
|
B |
AKT2 |
DDX58 |
GGT1 |
IFNAR1 |
JAK1 |
MAPK3 |
OAS2 |
PIK3R2 |
PPP2R2A |
RNASEL |
TBK1 |
GAPDH |
|
C |
AKT3 |
EGFR |
GPT |
IFNAR2 |
LDLR |
MAPK8 |
OAS3 |
PIK3R3 |
PPP2R2B |
RXRA |
TICAM1 |
LDHA |
|
D |
BAD |
EIF2AK1 |
GRB2 |
IFNB1 |
MAPK1 |
MAPK9 |
OCLN |
PIK3R5 |
PPP2R2C |
SCARB1 |
TLR3 |
NONO |
|
E |
CD81 |
EIF2AK2 |
HM13 |
IKBKB |
MAPK10 |
MAVS |
PDPK1 |
PPARA |
PPP2R2D |
SOS1 |
TNFRSF1A |
PPIH |
|
F |
CDKN1A |
EIF2AK3 |
IFIT1 |
IKBKE |
MAPK11 |
NFKB1 |
PIAS1 |
PPP2CA |
PSME3 |
SOS2 |
TRADD |
GDC |
|
G |
CHUK |
EIF2AK4 |
IFIT1B |
IKBKG |
MAPK12 |
NFKBIA |
PIK3CB |
PPP2CB |
RAF1 |
STAT1 |
TRAF2 |
PPC |
|
H |
CLDN1 |
EIF2S1 |
IFNA1 |
IRF1 |
MAPK13 |
NR1H3 |
PIK3CG |
PPP2R1A |
RELA |
STAT2 |
TYK2 |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK044 |
|
产地 |
美国 |
|
缩写 |
GQH-HPC |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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