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人转录误调节在恶性血液肿瘤中的定量PCR芯片试剂盒
英文名:GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Kit
货号:GK030
价格:¥4320.00
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人转录误调节在恶性血液肿瘤中的定量PCR芯片试剂盒描述:


       ScienCell's GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Kit (GQH-TMH) is designed to facilitate gene expression profiling of 88 key genes involved in three types hematological malignancy development (acute myeloid leukemia, precursor B lymphoblastic leukemia, and precursor T lymphoblastic leukemia), and is based on extensive literature research. Brief examples of how included genes may be grouped according to function and malignancy types are shown below:     

  • Cell cycle regulation: CCNT1, CCNT2, CDK9, CDK14, CDKN2C
  • Signaling mediation: IGF1R, BMP2K, PROM1
  • Differentiation regulation: CSF1R, CD14, DUSP6, GZMB, HOXA9, CCR7, FUT8, SIX4, EYA1
  • Mutated transcription factors identified
    • in acute myeloid leukemia: AML1, CEBPA, PLZF, ZBTB16, SPI1
    • in precursor B lymphoblastic leukemia: TCF3, PBX1
    • in precursor T lymphoblastic leukemia: TLX1, TLX3

       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis. 


       GeneQuery™ Human Transcriptional Misregulation in Hematological Malignancy qPCR Array Plate Layout*(8 controls in Bold and Italic)


 

1

2

3

4

5

6

7

8

9

10

11

12

A

AFF1

CCND2

CDKN1A

DUSP6

HDAC1

HPGD

KLF3

MEIS1

PBX1

RUNX2

TCF3

β-actin

B

ATM

CCNT1

CDKN2C

ETV6

HDAC2

IGF1

KMT2A

MLLT1

PER2

SIN3A

TLX1

GAPDH

C

BCL2A1

CCNT2

CEBPA

ETV7

HHEX

IGF1R

LDB1

MLLT3

PROM1

SIX1

TLX3

LDHA

D

BCL2L1

CCR7

CSF1R

EYA1

HIST2H3C

IL3

LMO2

MYC

PTCRA

SIX4

TRAF1

NONO

E

BCL6

CD14

CSF2

FCGR1A

HMGA2

ITGB7

LYL1

MYCN

RARA

SMAD1

UTY

PPIH

F

BIRC3

CD86

DDIT3

FUT8

HOXA10

JMJD1C

MAF

NCOR1

REL

SP1

WHSC1

GDC

G

BMP2K

CDK14

DEFA3

GRIA3

HOXA11

JUP

MAX

NFKB1

RUNX1

SPI1

WNT16

PPC

H

CCNA1

CDK9

DOT1L

GZMB

HOXA9

KDM6A

MEF2C

PAX5

RUNX1T1

SUPT3H

ZBTB16

NTC

* gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK030

产地

美国

缩写

GQH-TMH

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

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