人非小细胞肺癌定量PCR芯片试剂盒描述：

       ScienCell's GeneQuery™ Human Non-Small Cell Lung Cancer qPCR Array Kit (GQH-NLC) is designed to facilitate gene expression profiling of 88 key genes involved in non-small cell lung cancer (NSCLC) carcinogenesis. NSCLC accounts for more than 80% of all lung cancers. There are three main types of NSCLC: adenocarcinoma, lung squamous cell carcinoma and lung large cell carcinoma. Brief examples of how included genes may be grouped according to their functions are shown below:

       Oncogenes and tumor suppressor genes: KRAS, HRAS, EML4, ALK, RARB, FHIT, RASSF1, TP53, CDKN2A

       Cell cycle regulation and apoptosis: CDK4, CDK6, CCND1, CCNE2, CASP3, CASP9, BAD, STK4, FOXO3

       MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3

       PI3K/AKT pathway: PI3Ks, AKT1, AKT2

       Genes related to specific lung cancer types

       Adenocarcinoma: BRAF, ERBB2, TGFBR2, SMAD4, EPCAM, BIRC5

       Lung squamous cell carcinoma: FGFR2, CYPIA1, PTHLH, SOX2, PEBP1

       Lung large cell carcinoma: ROS1, NME1, TTN, MYO18B, EPHA5, WNK3

       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

       GeneQuery™ Human Non-Small Cell Lung Cancer qPCR Array Plate Layout*(8 controls in Bold and Italic)

* gene selection may be updated based on new research and development

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