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人胰腺星状细胞生物学定量PCR芯片试剂盒
英文名:GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Kit
货号:GK092
价格:¥4320.00
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人胰腺星状细胞生物学定量PCR芯片试剂盒描述:



       ScienCell's GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Kit (GQH-PSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human pancreatic stellate cell activation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) pancreatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:


       Pancreatic stellate cell markers: ACTA2, GFAP, COL11A1, DES, NGFR, POSTN, ITGA7, CTGF

       Pancreatic stellate cell activation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, CDH2, RARA, PDGFs, TGFB1, MMP2

       Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26s

       ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF

       Cytokine production: IL1A, IL1B, IL6, PDGFs, TGFBs, TNF

       p53 signaling pathway: CCNB1, CDK1, CCNE1, RRM2, GADD45G, CDK6, CHEK1, CDKN1A, EI24, ZMAT3

       Pancreatic fibrosis and cancer development: ACVR1B, CCKBR, CDKN2A, CELSR3, EGFR, GNL3, PALLD, RBBP8, RIMS1, SMAD4, STK11


       GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.


       GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Plate Layout*(8 controls in Bold and Italic)



 

1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

CDK1

COL2A1

DSP

HIF1A

LAMA2

MMP13

PALLD

RBP1

SPARC

THBS2

β-actin

B

ACTG2

CDK6

COL4A2

EGFR

ICAM1

LAMA3

MMP2

PDGFA

RBP2

SPP1

TIMP1

GAPDH

C

ACVR1B

CDKN1A

CRABP1

EI24

IL1A

LAMB1

MYH11

PDGFB

RBP4

STK11

TNC

LDHA

D

CCKBR

CDKN2A

CRABP2

FOXF1

IL1B

LAMB3

MYH9

PDGFC

RBP5

SYP

TNF

NONO

E

CCNB1

CELSR3

CTGF

GADD45G

IL6

LAMC1

NES

PDGFD

RELN

TGFB1

VCAN

PPIH

F

CCNE1

CHEK1

CYP26A1

GFAP

ITGA7

LHX2

NFKB1

POSTN

RIMS1

TGFB2

VIM

GDC

G

CDH1

CLEC3B

CYP26B1

GNL3

KLF6

LOX

NFKB2

RARA

RRM2

TGFB3

VTN

PPC

H

CDH2

COL11A1

DES

HAS1

LAMA1

LRAT

NGFR

RBBP8

SMAD4

TGFBI

ZMAT3

NTC


* gene selection may be updated based on new research and development


产品使用说明:仅供科研研究使用

货号

GK092

产地

美国

缩写

GQH-PSB

规格

One 96-well plate, one 20ul reaction per well

用途

科研

保存

4°C or -20°C

运输

常温

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