人肝星状细胞生物学定量PCR芯片试剂盒描述:
ScienCell's GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Kit (GQH-HSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human hepatic stellate cell activation and proliferation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) hepatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:
Hepatic stellate cell markers: ACTA2, GFAP, DES, NGFR, COL2A1, VIM, LAMA1, NES
Hepatic stellate cell activation and proliferation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, RARA, PDGFs, TGFB1, MMP2
Liver development and regeneration: STX2, PTN, SYNM, FOXF1, ICAM1, VCAM1, ITGB3, HGF, CXCL12, DES
Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26
ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF
Cytokine production: EGF, IGFs, PDGFs, FGFs, CSF1, CCL2, TNF, CXCL2, CXCL3, CXCL8
Hepatic fibrosis and cancer development: PKHD1, TMEM67, RPGRIP1L, SLC17A5, PKD1, CC2D2A, B9D1, B9D2
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Hepatic Stellate Cell Biology qPCR Array Plate Layout*(8 controls in Bold and Italic)
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
A |
ACTA2 |
COL2A1 |
CXCL2 |
FGF1 |
IGF1 |
LAMB1 |
MYH10 |
NGFR |
RARA |
SPARC |
TIMP1 |
β-actin |
B |
B9D1 |
COL4A2 |
CXCL3 |
FGF2 |
IGF2 |
LAMB3 |
MYH11 |
PDGFA |
RBP1 |
SPP1 |
TMEM67 |
GAPDH |
C |
B9D2 |
CRABP1 |
CXCL8 |
FOXF1 |
IL1A |
LAMC1 |
MYH9 |
PDGFB |
RBP2 |
STX2 |
TNC |
LDHA |
D |
BDNF |
CRABP2 |
CYP26A1 |
GFAP |
ITGB3 |
LEP |
NCAM1 |
PDGFC |
RBP4 |
SYNM |
TNF |
NONO |
E |
CC2D2A |
CSF1 |
CYP26B1 |
HAS1 |
KLF6 |
LHX2 |
NCAM2 |
PDGFD |
RBP5 |
SYP |
VCAM1 |
PPIH |
F |
CCL2 |
CTGF |
DES |
HGF |
LAMA1 |
LRAT |
NES |
PKD1 |
RELN |
TGFB1 |
VCAN |
GDC |
G |
CDH1 |
CXCL10 |
EGF |
ICAM1 |
LAMA2 |
MMP13 |
NFKB1 |
PKHD1 |
RPGRIP1L |
TGFBI |
VIM |
PPC |
H |
CLEC3B |
CXCL12 |
F2 |
IFNG |
LAMA3 |
MMP2 |
NFKB2 |
PTN |
SLC17A5 |
THBS2 |
VTN |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
货号
|
GK091 |
产地 |
美国 |
缩写 |
GQH-HSB |
规格 |
One 96-well plate, one 20ul reaction per well |
用途 |
科研 |
保存 |
4°C or -20°C |
运输 |
常温 |