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人甲状腺癌和疾病定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Thyroid Cancer and Disorders qPCR Array Kit (GQH-TCD) is designed to facilitate gene expression profiling of key genes involved in (i) the development of various types of thyroid cancer (papillary, follicular, and Hürthle cells); (ii) cell cycle regulation in the progression of thyroid cancer; and (iii) thyroid disorders, such as autoimmune thyroiditis and Hashimoto's thyroiditis. 88 genes are selected in this kit based on database and literature research.
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Thyroid Cancer and Disorders qPCR Array Plate Layout* (8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
AIRE |
CCNE2 |
CDK4 |
FAS |
HLA-DPA1 |
IFNA2 |
KRAS |
MYC |
PIK3CA |
RNF139 |
TFG |
β-actin |
|
B |
AKT1 |
CD28 |
CDKN1A |
FOXE1 |
HLA-DQA1 |
IFNA4 |
LEF1 |
NCOA4 |
PPARG |
RXRA |
TP53 |
GAPDH |
|
C |
BRAF |
CD40 |
CTLA4 |
GOLGA5 |
HLA-DRA |
IFNA5 |
LGALS3 |
NDUFA13 |
PRF1 |
RXRB |
TPM3 |
LDHA |
|
D |
C8orf4 |
CD80 |
CTNNB1 |
HLA-A |
HLA-E |
IFNA6 |
MAP2K1 |
NKX2-1 |
PRKAR1A |
RXRG |
TPR |
NONO |
|
E |
CALCA |
CD86 |
DIO2 |
HLA-B |
HLA-F |
IFNG |
MAP2K2 |
NNMT |
PTEN |
SERPINA7 |
TRIM24 |
PPIH |
|
F |
CCDC6 |
CDC23 |
EGF |
HLA-C |
HRAS |
IL2RA |
MAPK1 |
NRAS |
PTTG1 |
TCF7 |
TRIM27 |
GDC |
|
G |
CCNB1 |
CDH1 |
ERC1 |
HLA-DMA |
ICAM1 |
KDR |
MAPK3 |
PAX8 |
RASSF1 |
TCF7L1 |
TRIM33 |
PPC |
|
H |
CCND1 |
CDK2 |
FAM126A |
HLA-DOA |
IFNA1 |
KISS1R |
MINPP1 |
PCM1 |
RET |
TCF7L2 |
TSHB |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK014 |
|
产地 |
美国 |
|
缩写 |
GQH-TCD |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
|
参考文献 |
1] Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. (2002) "Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes." Genome Biol. 3(7): 1-12. |
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