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人内皮细胞表型异质性定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Kit (GQH-EPH) is designed to facilitate gene expression profiling of 88 key genes that are differentially expressed in vascular endothelial cells, and in endothelial cells from various tissues. Brief examples of how included genes may be grouped according to vascular types and tissue origins are shown below:
Endothelial cells vs. non-endothelial cells: PECAM1, CDH5, MMRN1, EFEMP1, VWF
Artery cells vs. vein cells: ALDH1A1, MPZL2, LIPG, ITM2A, CD44, MYO1B, GDF1, LEFTY1, SMO, EPHB4
Large vessel cells vs. microvessel cells: SPARC, JAM3, VEGFC, COL5A1, ROBO1, MYLK, COL4A3BP, EDNRB, VAV3, PDGFD
Tissue origin:
skin: FGF2, SCD, HMGCS1
lung: SFRP1, OGN
uterus: GAL
intestine: BTD
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Endothelial Cell Phenotypic Heterogeneity qPCR Array Plate Layout*(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ABLIM1 |
CD36 |
COL5A2 |
ESR2 |
GFRA2 |
ITGB4 |
LIPG |
MYO1B |
OGN |
PRKACA |
SPARC |
β-actin |
|
B |
ABLIM2 |
CD44 |
CTGF |
FABP4 |
GMFG |
ITM2A |
MCAM |
MYO5C |
PAK2 |
PROCR |
SSH1 |
GAPDH |
|
C |
ABLIM3 |
CDH5 |
EDN1 |
FABP5 |
HEY2 |
JAM3 |
MGP |
MYO7A |
PALMD |
ROBO1 |
TGFA |
LDHA |
|
D |
ALDH1A1 |
CEACAM1 |
EDNRB |
FABP7 |
HHIP |
KDR |
MMRN1 |
NAV1 |
PDGFD |
SCD |
THBS1 |
NONO |
|
E |
ANGPT2 |
COL4A1 |
EFEMP1 |
FGF2 |
HMGCS1 |
KRT7 |
MPZL2 |
NAV3 |
PECAM1 |
SEC14L1 |
TMSB4X |
PPIH |
|
F |
APOD |
COL4A2 |
EFNA1 |
GAL |
IL1R1 |
LAMA4 |
MRC1 |
NOTCH4 |
PGF |
SFRP1 |
VAV3 |
GDC |
|
G |
BMP6 |
COL4A3BP |
EPHB4 |
GDF1 |
IL6R |
LDB2 |
MRC2 |
NRG1 |
PODXL |
SMO |
VEGFC |
PPC |
|
H |
BTD |
COL5A1 |
ESM1 |
GFRA1 |
ITGA1 |
LEFTY1 |
MYLK |
NTN4 |
PPP1R16B |
SORBS2 |
VWF |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK018 |
|
产地 |
美国 |
|
缩写 |
GQH-EPH |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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