欢迎您访问中乔新舟官网!400-038-9959我的购物车(0)
您当前的位置:首页 > 产品中心
人脐静脉内皮细胞
英文名:Human Umbilical Vein Endothelial Cells
货号:8000
价格:¥5148.00
加入购物车,提交订单信息之后,我们会第一时间与您取得联系!
  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定
人脐静脉内皮细胞说明:

    

         血管内皮细胞对维持血管动态平衡起着重要作用。它们合成、分泌凝血和纤溶系统的激活因子和抑制因子、影响血小板粘附和聚集的调节因子。内皮细胞还释放控制细胞增殖和调节血管壁紧张度的分子。这些过程都可以用培养的细胞进行体外研究,人脐静脉和动脉内皮细胞是这类研究中常用的细胞类型。除了普通的内皮细胞特征例如“鹅卵石状”,VIII因子阳性着色,能摄取乙酰基化低密度脂蛋白,人脐血管内皮细CD31染色阳性。用IL-1或TNF-α预先处理过的细胞也能选择性地表达E-selectin和VCAM。    

       

        人脐静脉内皮细胞(HUVEC)提取于人脐带静脉组织,原代冻存。每管含有细胞数>5×105 cells/ml,此细胞通过vWF/Factor VIII 和CD31 (P-CAM)免疫荧光染色验证,经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到15倍增。


配套培养基:内皮细胞培养基(Cat.No.1001)


产品使用说明:仅供科研研究使用




货号 8000
产地 美国
缩写 HUVEC
规格 5 x 10^5 cells/vial
用途 科研
运输 干冰
保存 液氮
1.) Yan, X., Cao, J., Liang, L., Wang, L., Gao, F., Yang, Z., Duan, J., Chang, T., Deng, S., Liu, Y., Dou, G., Zhang, J., Zheng, Q., Zhang, P. & Han, H. (2016) miR-342-5p Is a Notch Downstream Molecule and Regulates Multiple Angiogenic Pathways Including Notch, Vascular Endothelial Growth Factor and Transforming Growth Factor ? SignalingJ Am Heart Assoc. 5

2.) He, Y., Luan, Z., Fu, X. & Xu, X. (2016) Overexpression of uncoupling protein 2 inhibits the high glucose-induced apoptosis of human umbilical vein endothelial cells Int J Mol Med. 37

3.) Ye, X., Qian, Y., Wang, Q., Yuan, W., Mo, X., Li, Y., Jiang, Z., Xu, W., Deng, Y., Wan, Y., Fan, X., Wu, X. & Wang, Y. (2016) SMYD1, an SRF-Interacting Partner, Is Involved in Angiogenesis PLoS One. 11

4.) Zhou, Y., Zhang, H., Zheng, B., Ye, L., Zhu, S., Johnson, N.R., Wang, Z., Wei, X., Chen, D., Cao, G., Fu, X., Li, X., Xu, H.Z. & Xiao, J. (2016) Retinoic Acid Induced-Autophagic Flux Inhibits ER-Stress Dependent Apoptosis and Prevents Disruption of Blood-Spinal Cord Barrier after Spinal Cord Injury Int J Biol Sci. 12

5.) Wang, Y., Wang, B., Guerram, M., Sun, L., Shi, W., Tian, C., Zhu, X., Jiang, Z. & Zhang, L. (2015) Deoxypodophyllotoxin suppresses tumor vasculature in HUVECs by promoting cytoskeleton remodeling through LKB1-AMPK dependent Rho A activation Oncotarget. 6

6.) Wang, K., Chen, X., Pan, Y., Cui, Y., Zhou, X., Kong, D. & Zhao, Q. (2015) Enhanced Vascularization in Hybrid PCL/Gelatin Fibrous Scaffolds with Sustained Release of VEGF Biomed Res Int. 2015

7.) Li, J., Zhou, J., Zhang, D., Song, Y., She, J. & Bai, C. (2015) Bone marrow-derived mesenchymal stem cells enhance autophagy via PI3K/AKT signalling to reduce the severity of ischaemia/reperfusion-induced lung injury J Cell Mol Med. 19

8.) Lau, Y.S., Ling, W.C., Murugan, D., Kwan, C.Y. & Mustafa, M.R. (2015) Endothelium-Dependent Relaxation Effect of Apocynum venetum Leaf Extract via Src/PI3K/Akt Signalling Pathway Nutrients. 7

9.) Jiang, Y.H., Sun, W., Li, W., Hu, H.Z., Zhou, L., Jiang, H.H. & Xu, J.X. (2015) Calycosin-7-O-?-D-glucoside promotes oxidative stress-induced cytoskeleton reorganization through integrin-linked kinase signaling pathway in vascular endothelial cells BMC Complement Altern Med. 15

10.) Dissanayaka, W.L., Hargreaves, K.M., Jin, L., Samaranayake, L.P. & Zhang, C. (2015) The Interplay of Dental Pulp Stem Cells and Endothelial Cells in an Injectable Peptide Hydrogel on Angiogenesis and Pulp Regeneration In Vivo Tissue Eng Part A. 21

11.) Deng, M., Gu, Y., Liu, Z., Qi, Y., Ma, G.E. & Kang, N. (2015) Endothelial Differentiation of Human Adipose-Derived Stem Cells on Polyglycolic Acid/Polylactic Acid MeshStem Cells Int. 2015

12.) Yang, B., Cai, B., Deng, P., Wu, X., Guan, Y., Zhang, B., Cai, W., Schaper, J. & Schaper, W. (2015) Nitric Oxide Increases Arterial Endotheial Permeability through Mediating VE-Cadherin Expression during Arteriogenesis PLoS One. 10

13.) Yang, G., Li, Y., Wu, W., Liu, B., Ni, L., Wang, Z., Miao, S., Wang, L. & Liu, C. (2015) Anti-oxidant effect of heme oxygenase-1 on cigarette smoke-induced vascular injuryMol Med Rep. 12

14.) Ye, Q., Qin, S., Liu, Y., Feng, J., Wu, Q., Qu, W. & Yin, X. (2015) Inhibitory Effect of Endostar on Specific Angiogenesis Induced by Human Hepatocellular CarcinomaGastroenterol Res Pract. 2015

15.) Yuan, C., Wang, P., Zhu, L., Dissanayaka, W.L., Green, D.W., Tong, E.H., Jin, L. & Zhang, C. (2015) Coculture of Stem Cells from Apical Papilla and Human Umbilical Vein Endothelial Cell Under Hypoxia Increases the Formation of Three-Dimensional Vessel-Like Structures in Vitro Tissue Eng Part A. 21

16.) Zhang, E., Guo, Q., Gao, H., Xu, R., Teng, S. & Wu, Y. (2015) Metformin and Resveratrol Inhibited High Glucose-Induced Metabolic Memory of Endothelial Senescence through SIRT1/p300/p53/p21 Pathway PLoS One. 10

17.) Zhao, J., Geng, Y., Hua, H., Cun, B., Chen, Q., Xi, X., Yang, L. & Li, Y. (2015) Fenofibrate inhibits the expression of VEGFC and VEGFR-3 in retinal pigmental epithelial cells exposed to hypoxia Exp Ther Med. 10

18.) Guo W, Zhang D, Wang X, Kong W, Zhang Q, Zhang Y, Liu W. (2013) "Actin cytoskeleton modulates ADMA-induced NF-kappaB nuclear translocation and ICAM-1 expression in endothelial cells." Med Sci Monit. 17: BR242

19.) Yu Y, Yang P, Chuah Q, Huang Y, Peng C, Lee Y, Chiu S. (2013) "Radiation-induced senescence in securin-deficient cancer cells promotes cell invasion involving the IL-6/STAT3 and PDGF-BB/PDGFR pathways." Sci Rep. 3: 1675

20.) Chen G, Zhao L, Feng J, You G, Sun Q, Li P, Han D, Zhou H. (2013) "Validation of Reliable Reference Genes for Real-Time PCR in Human Umbilical Vein Endothelial Cells on Substrates with Different Stiffness." PLoS One. 8: e67360

21.) Aisha AF, Ismail Z, Abu-Salah KM, Siddiqui JM, Ghafar G, Abdul Majid AM. (2013) "Syzygium campanulatum korth methanolic extract inhibits angiogenesis and tumor growth in nude mice" BMC Complement Altern Med. 13: 168. 

22.) Pan Q, Pan H, Lou H, Xu Y, Tian L. (2013) "Inhibition of the angiogenesis and growth of Aloin in human colorectal cancer in vitro and in vivo." Cancer Cell Int. 13: 69.

23.) Cheng HS, Sivachandran N, Lau A, Boudreau E, Zhao JL, Baltimore D, Delgado-Olguin P, Cybulsky MI, Fish JE. (2013) "MicroRNA-146 represses endothelial activation by inhibiting pro-inflammatory pathways." EMBO Mol Med. 5: 1017-34. 

24.) Hou, Y., Zou, Q., Ge, R., Shen, F. & Wang, Y. (2012) The critical role of CD133+CD44+/high tumor cells in hematogenous metastasis of liver cancers Cell Res. 22

25.) Huiyong Z, Yong L, Didier M, Yu Z, Jing F, Rongyue C, Jingjing L. (2011) "Enhanced inhibition of murine prostatic carcinoma growth by immunization with or administration of viable human umbilical vein endothelial cells and CRM197." Braz J Med Biol Res. 44: 140-8.

26.) Zhao Y, Yu L, Xu S, Qiu F, Fan Y, Fu G. (2011) "Down-regulation of connexin43 gap junction by serum deprivation in human endothelial cells was improved by (-)-Epigallocatechin gallate via ERK MAP kinase pathway." Biochem Biophys Res Commun. 404: 217-22.

27.) Ghosh C, Gonzalez-Martinez J, Hossain M, Cucullo L, Fazio V, Janigro D, Marchi N. (2010) "Pattern of P450 expression at the human blood-brain barrier: roles of epileptic condition and laminar flow." Epilepsia. 51: 1408-17.

28.) Fang W, Li H, Zhou L, Su L, Liang Y, Mu Y. (2010) "Effect of prostaglandin E1 on TNF-induced vascular inflammation in human umbilical vein endothelial cells." Can J Physiol Pharmacol. 88: 576-83.

29.) Gao ZX, Huang DY, Li HX, Zhang LN, Lv YH, Cui HD, Zheng JH. (2010) "Scutellarin promotes in vitro angiogenesis in human umbilical vein endothelial cells." Biochem Biophys Res Commun. 400: 151-6.

30.) Ou Y, Guo XL, Zhai L, Liu XY, Cheng YN. (2010) "TMPDP, a tetramethylpyrazine derivative, protects vascular endothelial cells from oxidation damage by hydrogen peroxide." Pharmazie. 65: 755-9.

31.) Austin SA, Sens MA, Combs CK. (2009) "Amyloid precursor protein mediates a tyrosine kinase-dependent activation response in endothelial cells." J Neurosci. 29: 14451-62.

32.) Cueni LN, Detmar M. (2009) "Galectin-8 interacts with podoplanin and modulates lymphatic endothelial cell functions." Exp Cell Res. 315: 1715-23. 

33.) Li Y, Song YH, Li F, Yang T, Lu YW, Geng YJ. (2009) "MicroRNA-221 regulates high glucose-induced endothelial dysfunction." Biochem Biophys Res Commun. 381: 81-3.

34.) Vigl B, Zgraggen C, Rehman N, Banziger-Tobler NE, Detmar M, Halin C. (2009) "Coxsackie- and adenovirus receptor (CAR) is expressed in lymphatic vessels in human skin and affects lymphatic endothelial cell function in vitro." Exp Cell Res. 315: 336-47.

35.) Xiong YQ, Sun HC, Zhang W, Zhu XD, Zhuang PY, Zhang JB, Wang L, Wu WZ, Qin LX, Tang ZY. (2009) "Human hepatocellular carcinoma tumor-derived endothelial cells manifest increased angiogenesis capability and drug resistance compared with normal endothelial cells." Clin Cancer Res. 15: 4838-46. 

36.) Cucullo L, Couraud PO, Weksler B, Romero IA, Hossain M, Rapp E, Janigro D. (2008) "Immortalized human brain endothelial cells and flow-based vascular modeling: a marriage of convenience for rational neurovascular studies." J Cereb Blood Flow Metab. 28: 312-28.

37.) Yin W, Ghebrehiwet B, Weksler B, Peerschke EI. (2008) "Regulated complement deposition on the surface of human endothelial cells: effect of tobacco smoke and shear stress." Thromb Res. 122: 221-8.

38.) Gupta GP, Perk J, Acharyya S, de Candia P, Mittal V, Todorova-Manova K, Gerald WL, Brogi E, Benezra R, Massagu? J. (2007) "ID genes mediate tumor reinitiation during breast cancer lung metastasis." Proc Natl Acad Sci USA. 104: 19506-11.

39.) Ng ES, Cheng ZJ, Ellis A, Ding H, Jiang Y, Li Y, Hollenberg MD, Triggle CR. (2007) "Nitrosothiol stores in vascular tissue: Modulation by ultraviolet light, acetylcholine and ionomycin." Eur J Pharmacol. 560: 183-92. 

40.) Yin W, Ghebrehiwet B, Weksler B, Peerschke EI. (2006) "Classical pathway complement activation on human endothelial cells." Mol Immunol. 44: 2228-34.

41.) Ray RB, Basu A, Steele R, Beyene A, McHowat J, Meyer K, Ghosh AK, Ray R. (2004) "Ebola virus glycoprotein-mediated anoikis of primary human cardiac microvascular endothelial cells." Virology. 321: 181-8.

42.) D?m?t?r E, Benzakour O, Griffin JH, Yule D, Fukudome K, Zlokovic BV. (2003) "Activated protein C alters cytosolic calcium flux in human brain endothelium via binding to endothelial protein C receptor and activation of protease activated receptor-1." Blood. 101: 4797-801.
Question:
Greetings! I am the biosafety officer at our institution, and we've had a researcher order primary HUVEC cells from your company. Would you be able to let us know if you test the cultures/isolates for infectious agents or viruses and what agents you test for?

Answers:
Posted by Manoj Sharma on Thursday, August 20, 2015
Each cell strain tests negative for HIV, HBV and HCV. However, keep in mind that diagnostic tests are not necessarily 100% accurate, therefore, proper precautions must be taken to avoid inadvertent exposure. Always wear gloves and safety glasses when working with these materials. Never mouth pipette. We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination.
Grizzle WE, Polt S. (1988) “Guidelines to avoid personal contamination by infective agents in research laboratories that use human tissues.” J Tissue Culture Methods. 11: 191-9
Please reply here if you have further questions
Best,
ScienCell Team

公司简介 / Company profile
上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
           上海中乔新舟生物科技有限公司(官网:www.zqxzbio.com)成立于2011年,历经...
联系我们 / Contact
电 话:021-56760357;021-56760351
传 真:
邮 箱:sales@zqxzbio.com
邮 编:200439
地 址:上海市宝山区长江南路180号
Copyright © 2014 ZQXZBIO All rights reserved.
技术支持:中乔新舟沪ICP备14008091号