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人小细胞肺癌定量PCR芯片试剂盒描述：
ScienCell's GeneQuery™ Human Small Cell Lung Cancer qPCR Array Kit (GQH-SLC) is designed to facilitate gene expression profiling of 88 key genes involved in small cell lung cancer (SCLC) carcinogenesis. SCLC accounts for approximately 10-20% of all lung cancers. Compared to non-small cell lung cancer (NSCLC), SCLC usually grows and metastasizes more rapidly. Brief examples of how included genes may be grouped according to their functions are shown below:
Oncogenes and tumor suppressor genes: MYC, RARB, FHIT, TP53, PTEN
Cell cycle regulation and apoptosis: CDK2, CDK4, CDK6, CCND1, CCNE2, E2F2, CASP9
PI3K/AKT pathway: PI3Ks, AKT1, AKT2
IKK/NFKB pathway: NFKB1, CHUK, IKBKB
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
GeneQuery™ Human Small Cell Lung Cancer qPCR Array Plate Layout*(8 controls in Bold and Italic)
|
|
1 |
2 |
3 |
4 |
5 |
6 |
7 |
8 |
9 |
10 |
11 |
12 |
|
A |
ABCB1 |
CCND1 |
CHUK |
CSF3 |
HTATIP2 |
KRT19 |
LAMB2 |
MYCL |
PIK3CA |
PTK2 |
SYP |
β-actin |
|
B |
ABCC1 |
CCNE2 |
CKS1B |
CXCR2 |
IKBKB |
KRT20 |
LAMB3 |
NCAM1 |
PIK3CD |
RARB |
TOP1 |
GAPDH |
|
C |
AKT1 |
CDK2 |
CKS2 |
CYCS |
ITGA2 |
KRT7 |
LAMB4 |
NFKB1 |
PIK3CG |
RASSF1 |
TP53 |
LDHA |
|
D |
AKT2 |
CDK4 |
COL4A1 |
E2F2 |
ITGA2B |
LAMA1 |
LAMC1 |
NKX2-1 |
PIK3R1 |
RCVRN |
TRAF1 |
NONO |
|
E |
APAF1 |
CDK6 |
COL4A2 |
EGFR |
ITGA3 |
LAMA2 |
LAMC2 |
NMB |
PIK3R3 |
RXRA |
TRAF4 |
PPIH |
|
F |
BIRC2 |
CDKN1B |
COL4A4 |
ENO2 |
ITGAV |
LAMA3 |
LAMC3 |
NMBR |
PIK3R5 |
RXRG |
TRAF5 |
GDC |
|
G |
BIRC7 |
CDKN2A |
COL4A5 |
FHIT |
ITGB1 |
LAMA5 |
MAX |
NOS2 |
PTEN |
SKP2 |
TRAF6 |
PPC |
|
H |
CASP9 |
CHGA |
COL4A6 |
FN1 |
KIT |
LAMB1 |
MYC |
PIAS2 |
PTGS2 |
SST |
XIAP |
NTC |
* gene selection may be updated based on new research and development
产品使用说明:仅供科研研究使用
|
货号 |
GK032 |
|
产地 |
美国 |
|
缩写 |
GQH-SLC |
|
规格 |
One 96-well plate, one 20ul reaction per well |
|
用途 |
科研 |
|
保存 |
4°C or -20°C |
|
运输 |
常温 |
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