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大鼠ICAM-1ELISA试剂盒
英文名:Rat ICAM-1 ELISA Kit
货号:EK0372
价格:¥7020.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

大鼠ICAM-1ELISA试剂盒描述:


       Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of ICAM-1 is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells, and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain has 493 amino acids with the molecular mass of 78 kDa. As a result of glycosylation, the molecular mass of 110-125 KDa is revealed by SDS-PAGE. ScienCell's rat ICAM-1 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Rat ICAM-1-specific monoclonal antibodies are pre-coated onto 8 x 12 divisible strips. The rat specific detection polyclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of rat ICAM-1 captured in strips.


产品使用说明:仅供科研研究使用

货号

EK0372

产地

美国

缩写

rICAM-1-ELISA

规格

96 tests

用途

科研

保存

频繁使用存储在4°C,很少使用存储在-20°C

运输

胶冰

参考文献

1. Ballantyne, C. M.; Kozak, C. A.; O'Brien, W. E.; Beaudet, A. L. Assignment of the gene for intercellular adhesion molecule-1 (Icam-1) to proximal mouse chromosome 9. Genomics 9: 547-550, 1991.
2. Bella, J.; Kolatkar, P. R.; Marlor, C. W.; Greve, J. M.; Rossmann, M. G. The structure of the two amino-terminal domains of human ICAM-1 suggests how it functions as a rhinovirus receptor and as an LFA-1 integrin ligand. Proc. Nat. Acad. Sci. 95: 4140-4145, 1998.
3. Simmons, D.; Makgoba, M. W.; Seed, B. ICAM, an adhesion ligand of LFA-1, is homologous to the neural cell adhesion molecule NCAM. Nature 331: 624-627, 1988

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