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MV4-11人急性单核细胞白血病细胞 (STR鉴定)
英文名:MV4-11
货号:ZQ0464
价格:¥1600.00
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MV4-11人急性单核细胞白血病细胞 (STR鉴定)

¥1600.00
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MV-4-11人急性单核细胞白血病细胞专用培养基

¥350.00 ¥500.00

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细胞套餐惊爆价

¥1950 ¥2100.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

MV4-11人急性单核细胞白血病细胞

货号

ZQ0464

产品介绍

MV-4-11细胞系由Rovera课题组建立,来源于一名患有人双表型髓性单核细胞白血病(biphenotypic B myelomonocytic leukemia)的10岁男孩的外周血。Interleukin (IL)-3可以独立地支持该细胞长期生长,使用10%FBS培养时则不需要额外添加IL-3。但是,在IL-3和生长因子Granulocyte/Macrophage Colony-Stimulating Factor (GM-CSF)均处于低浓度的情况下,IL-3会抑制MV-4-11细胞的增殖。生长因子Granulocyte Colony Stimulating Factor(G-CSF)会协同GM-CSF促进MV-4-11细胞的增殖,而单独的G-CSF会短暂刺激该细胞系。据文献[PubMed: 3500218]报道,间接免疫荧光法检测髓性单核细胞抗原CD15,超过96%的该细胞为阳性,40~96%为单核细胞抗原CD4阳性,4-11%为单核细胞抗原CD10阳性。

【注意事项】:
a) 该细胞为悬浮细胞,根据培养经验以及客户的反馈,传代时使用【半换液法】对细胞状态较为有利,因此我库建议您使用【半换液法】进行传代。同时,您在收到细胞后,请不要通过离心的方式收集细胞,可以直接向培养瓶中添加等体积的新鲜培养液,然后将细胞吹打均匀后移入两个新的T25培养瓶中继续培养即可。
b) 细胞对血清质量较为敏感,建议您使用优质血清进行培养。
c) 该细胞对细胞密度较为敏感,培养、传代时请注意保持细胞密度在合适的范围。细胞接种密度20万个/mL ,培养时维持细胞密度在10万-100万个/mL

种属

性别/年龄

男/10岁

组织

外周血

疾病

双表型B粒单核细胞白血病

细胞类型

巨噬细胞

形态学

成淋巴细胞

生长方式

悬浮

倍增时间

大约32-50小时 

培养基和添加剂

IMDM(品牌:中乔新舟  货号:ZQ-900+15%胎牛血清(中乔新舟 货号:ZQ0500+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0464

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 13

D16S539: 11,12

D5S818: 11,12

D7S820: 8,9

THO1: 8,9.3

TPOX: 8,11

vWA: 14,15

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 

基因表达

 

保藏机构

ATCC; CRL-9591 DSMZ; ACC-102

供应限制

仅供科研使用

货号

ZQ0464

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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