欢迎您访问中乔新舟官网!400-038-9959我的购物车(0)
您当前的位置:首页 > 产品中心
Hela人宫颈癌细胞(STR鉴定)[套餐促销]
英文名:Hela
货号:ZQ0068
价格:¥1350.00
加入购物车,提交订单信息之后,我们会第一时间与您取得联系!
推荐组合

Hela人宫颈癌细胞(STR鉴定)[套餐促销]

¥1350.00
+

HeLa人宫颈癌细胞专用培养基

¥350.00 ¥480.00

配套完培,组合使用,省时省力

=

细胞套餐惊爆价

¥1700 ¥1880.00
加入购物车
  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

Hela人宫颈癌细胞

货号

ZQ0068

产品介绍

HeLa细胞系是从一位31岁黑人女性患者的宫颈癌组织中分离和培养出来的,这是世界上第一株体外培养的人类永生化细胞株。角蛋白免疫过氧化物酶染色阳性。 据报道,p53表达水平低,pRB(成视网膜细胞瘤抑制因子)表达水平正常。Hela 为重大研究进展做出了重大贡献,并继续在世界各地的实验室中发挥着关键作用。
1951 年,亨利埃塔·拉克斯 (Henrietta Lacks) 是一位五个孩子的年轻母亲,因阴道出血前往约翰·霍普金斯医院求医,霍华德·琼斯 (Howard Jones) 医生在她的子宫颈上发现了一个严重的恶性肿瘤。当时,约翰霍普金斯大学医学研究所是为数不多的为贫困非裔美国人提供医疗服务的机构之一。亨利埃塔·拉克斯 (Henrietta Lacks) 接受了针对宫颈癌的镭治疗,这是当时最先进的治疗方法。在她的治疗期间,进行了活组织检查,并将她的癌细胞样本送往乔治·奥托·盖伊博士的实验室。盖伊博士一直试图培养来自不同背景的宫颈癌患者的细胞,但一直没有成功,直到亨利埃塔的细胞成为第一个持续增殖的细胞,这一发现使它们与之前的所有样本区分开来。后来发现亨丽埃塔·拉克斯的宫颈癌是由人乳头瘤病毒(HPV)引起的。 HPV 是一种常见病毒,可导致宫颈癌等疾病。对 HeLa 细胞的研究极大地促进了了解 HPV 在宫颈癌中的作用,从而促进了预防性 HPV 疫苗的开发,这对降低 HPV 相关癌症的发病率产生了深远的影响。
该细胞以 Henrietta Lacks 的名字缩写命名为“HeLa”细胞,从此在医学研究中发挥了重要作用。它们使科学家能够研究癌细胞的生长、各种物质的影响以及病毒的作用,从而极大地促进了医学进步,包括开发脊髓灰质炎和 COVID-19 疫苗,而无需担心直接人体实验的伦理问题。HeLa细胞因其高转染效率和对病毒感染的敏感性而被广泛用于基因功能研究、重组蛋白生产和基因治疗。在研究病毒行为(包括复制和发病机制)方面发挥着关键作用,并通过表达病毒蛋白并帮助开发诊断测试和疫苗,在乙型肝炎研究中发挥了关键作用,从而显着推进了全球卫生措施。
Hela细胞仍然是正在进行的医学和科学研究的宝贵资源。 HeLa 细胞和其他永生细胞系的重要性怎么强调都不为过,因为它们继续塑造医学和传染病研究领域,并且代表了 Henrietta Lacks 的持久遗产及其对科学进步的贡献。

注意事项】:该细胞代谢旺盛,建议及时换液或传代。

种属

性别/年龄

女/31岁

组织

子宫颈

疾病

宫颈腺癌

细胞类型

肿瘤细胞

形态学

上皮细胞

生长方式

贴壁

倍增时间

大约31.2~48小时

培养基和添加剂

EMEM(含NEAA)(品牌:中乔新舟 货号:ZQ-300)++10%FBS(品牌:中乔新舟 货号:ZQ500-A)+1%P/S(中乔新舟 货号:CSP006

推荐完全培养基货号

ZM0068

生物安全等级

BSL-2

STR位点信息

Amelogenin: X

CSF1PO: 9,10

D13S317: 12,13.3

D16S539: 9,10

D5S818: 11,12

D7S820: 8,12

TH01: 7

TPOX: 8,12

vWA: 16,18

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

  ***

基因表达

  ***

保藏机构

ATCC; CCL-2 ATCC; CRM-CCL-2 ATCC; CRL-7923 BCRC; 60005 BCRJ; 0100 DSMZ; ACC-57 ECACC; 08011102 ECACC; 93021013

供应限制

仅供科研使用

货号

ZQ0068

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


论文标题: Photodegradation of carbon dots cause cytotoxicity
DOI: 10.1038/s41467-021-21080-z
发表时间: 2021-02-05
期刊: Nature Communications
影响因子: 14.919
货号: ZQ0068
产品名称: HeLa cells
原文链接: https://www.nature.com/articles/s41467-021-21080-z


论文标题: Photococatalytic anticancer performance of naked Ag/AgCl nanoparticles
DOI: 10.1016/j.cej.2021.131265
发表时间: 2021-07-10
期刊: CHEMICAL ENGINEERING JOURNAL
影响因子: 13.273
货号: ZQ0068
产品名称: HeLa cells
原文链接: https://www.sciencedirect.com/science/article/pii/S1385894721028461


论文标题: Enhanced singlet oxygen production over a photocatalytic stable metal organic framework composed of porphyrin and Ag
DOI: 10.1016/j.jcis.2021.05.087
发表时间: 2021-05-18
期刊: JOURNAL OF COLLOID AND INTERFACE SCIENCE
影响因子: 8.128
货号: ZQ0068
产品名称: HeLa cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0021979721007608


论文标题: A label-free technique to quantify and visualize gold nanoparticle accumulation at the single-cell level
DOI: 10.1016/j.chemosphere.2022.134857
发表时间: 2022-05-10
期刊: CHEMOSPHERE
影响因子: 7.086
货号: ZQ0068
产品名称: HeLa cells

原文链接: https://www.sciencedirect.com/science/article/pii/S0045653522013509


论文标题: Dual-sensitized Eu(III)/Tb(III) complexes exhibiting tunable luminescence emission and their application in cellular-imaging
DOI: 10.1039/D2DT00051B
发表时间: 2022-01-24
期刊: DALTON TRANSACTIONS
影响因子: 4.569
货号: ZQ0068
产品名称: HeLa cells
原文链接: https://pubs.rsc.org/en/content/articlelanding/2022/dt/d2dt00051b
 

论文标题: Targeted Antitumor Mechanism of C-PC/CMC-CD55sp Nanospheres in HeLa Cervical Cancer Cells
DOI: 10.3389/fphar.2020.00906
发表时间: 2020-06-18
期刊: Frontiers in Pharmacology
影响因子: 4.225
货号: ZQ0068
产品名称: HeLa cells
原文链接: https://www.frontiersin.org/articles/10.3389/fphar.2020.00906/full

论文标题: Overexpression of SMC4 predicts a poor prognosis in cervical cancer and facilitates cancer cell malignancy phenotype by activating NF-κB pathway
DOI: 10.1007/s13577-021-00603-2
发表时间: 2021-09-03
期刊: Human Cell
影响因子: 4.174
货号: ZQ0068
产品名称: HeLa cells
原文链接: https://link.springer.com/article/10.1007/s13577-021-00603-2


论文标题: Synthesis of α-indolylacrylates as potential anticancer agents using a Brønsted acid ionic liquid catalyst and the butyl acetate solvent
DOI: 10.1039/D0RA00990C
发表时间: 2020-04-02
期刊: RSC Advances
影响因子: 3.119
货号: ZQ0068
产品名称: HeLa cells
原文链接: https://pubs.rsc.org/en/content/articlelanding/2020/ra/d0ra00990c


论文标题: Cationic Albumin Encapsulated DNA Origami for Enhanced Cellular Transfection and Stability
DOI: 10.3390/ma12060949
发表时间: 2019-03-21
期刊: Materials
影响因子: 2.972
货号: ZQ0068
产品名称: Hela cell line
原文链接: https://www.mdpi.com/431980


论文标题: EGFR and ERK activation resists flavonoid quercetin‑induced anticancer activities in human cervical cancer cells <em>in vitro</em>
DOI: 10.3892/ol.2021.13015
发表时间: 2021-08-31
期刊: Oncology Letters
影响因子: 2.967
货号: ZQ0068
产品名称: HeLa cells

原文链接: https://www.spandidos-publications.com/10.3892/ol.2021.13015



CLPUB00047
Gey G.O., Coffman W.D., Kubicek M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res. 12:264-265(1952)


PubMed=13052828; DOI=10.1084/jem.97.5.695
Scherer W.F., Syverton J.T., Gey G.O.
Studies on the propagation in vitro of poliomyelitis viruses. IV Viral multiplication in a stable strain of human malignant epithelial cells (strain HeLa) derived from an epidermoid carcinoma of the cervix.
J. Exp. Med. 97:695-710(1953)


PubMed=13207312
Scherer W.F., Syverton J.T.
The viral range in vitro of a malignant human epithelial cell (strain HeLa, Gey). I. Multiplication of herpes simplex, pseudorabies, and vaccinia viruses.
Am. J. Pathol. 30:1057-1073(1954)


PubMed=13261081
Moore A.E., Sabachewsky L., Toolan H.W.
Culture characteristics of four permanent lines of human cancer cells.
Cancer Res. 15:598-602(1955)


PubMed=16589695; DOI=10.1073/pnas.41.7.432
Puck T.T., Marcus P.I.
A rapid method for viable cell titration and clone production with HeLa cells in tissue culture: the use of X-irradiated cells to supply conditioning factors.
Proc. Natl. Acad. Sci. U.S.A. 41:432-437(1955)


PubMed=14031339
Katzberg A.A.
The effect of space flights on living human cells aboard Discoverer XVIII.
Tech. Doc. Rep. SAMTDR USAF Sch. Aerosp. Med. 62:43.1-43.4(1962)


PubMed=14185313; DOI=10.1126/science.146.3641.241
Cell Culture Collection Committee
Animal cell strains. The Cell Culture Collection Committee has assembled and certified 23 strains of animal cells.
Science 146:241-243(1964)


PubMed=5668122; DOI=10.3181/00379727-128-33119
Peterson W.D. Jr., Stulberg C.S., Swanborg N.K., Robinson A.R.
Glucose-6-phosphate dehydrogenase isoenzymes in human cell cultures determined by sucrose-agar gel and cellulose acetate zymograms.
Proc. Soc. Exp. Biol. Med. 128:772-776(1968)


DOI=10.1007/BF02618370
Stulberg C.S., Coriell L.L., Kniazeff A.J., Shannon J.E.
The animal cell culture collection.
In Vitro 5:1-16(1970)


PubMed=4942173; DOI=10.1097/00006250-197112000-00028
Jones H.W. Jr., McKusick V.A., Harper P.S., Wuu K.-D.
George Otto Gey (1899-1970). The HeLa cell and a reappraisal of its origin.
Obstet. Gynecol. 38:945-949(1971)


DOI=10.1007/978-1-4757-1647-4_13
Biedler J.L.
Chromosome abnormalities in human tumor cells in culture.
(In) Human tumor cells in vitro; Fogh J. (eds.); pp.359-394; Springer; New York (1975)


PubMed=1246620; DOI=10.1126/science.1246620
Hsu S.H., Schacter B.Z., Delaney N.L., Miller T.B., McKusick V.A., Kennett R.H., Bodmer J.G., Young D., Bodmer W.F.
Genetic characteristics of the HeLa cell.
Science 191:392-394(1976)


PubMed=77569; DOI=10.1111/j.1399-0039.1978.tb01259.x
Espmark J.A., Ahlqvist-Roth L., Sarne L., Persson A.
Tissue typing of cells in culture. III. HLA antigens of established human cell lines. Attempts at typing by the mixed hemadsorption technique.
Tissue Antigens 11:279-286(1978)


PubMed=6256643; DOI=10.1038/288724a0
Day R.S. III, Ziolkowski C.H.J., Scudiero D.A., Meyer S.A., Lubiniecki A.S., Girardi A.J., Galloway S.M., Bynum G.D.
Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains.
Nature 288:724-727(1980)


PubMed=6935474; DOI=10.1093/jnci/66.2.239
Wright W.C., Daniels W.P., Fogh J.
Distinction of seventy-one cultured human tumor cell lines by polymorphic enzyme analysis.
J. Natl. Cancer Inst. 66:239-247(1981)


PubMed=6358339; DOI=10.1093/jhmas/38.4.415
Brown R.W., Henderson J.H.M.
The mass production and distribution of HeLa cells at Tuskegee Institute, 1953-55.
J. Hist. Med. Allied Sci. 38:415-431(1983)


PubMed=6401685; DOI=10.1007/BF02617989
Halton D.M., Peterson W.D. Jr., Hukku B.
Cell culture quality control by rapid isoenzymatic characterization.
In Vitro 19:16-24(1983)


PubMed=6825208; DOI=10.1093/carcin/4.2.199
Yarosh D.B., Foote R.S., Mitra S., Day R.S. III
Repair of O6-methylguanine in DNA by demethylation is lacking in Mer- human tumor cell strains.
Carcinogenesis 4:199-205(1983)


PubMed=2983228; DOI=10.1038/314111a0
Schwarz E., Freese U.-K., Gissmann L., Mayer W., Roggenbuck B., Stremlau A., zur Hausen H.
Structure and transcription of human papillomavirus sequences in cervical carcinoma cells.
Nature 314:111-114(1985)


PubMed=2990217
Yee C., Krishnan-Hewlett I., Baker C.C., Schlegel R., Howley P.M.
Presence and expression of human papillomavirus sequences in human cervical carcinoma cell lines.
Am. J. Pathol. 119:361-366(1985)



CLPUB00262

Gold M.
A conspiracy of cells. One woman's immortal legacy and the medical scandal it caused.
(In) ISBN 9780887060991; pp.1-171; State University of New York Press; Albany (1986)



PubMed=3518877; DOI=10.3109/07357908609038260
Fogh J.
Human tumor lines for cancer research.
Cancer Invest. 4:157-184(1986)


PubMed=3028716; DOI=10.1159/000132342
Popescu N.C., DiPaolo J.A., Amsbaugh S.C.
Integration sites of human papillomavirus 18 DNA sequences on HeLa cell chromosomes.
Cytogenet. Cell Genet. 44:58-62(1987)


PubMed=3180844; DOI=10.1159/000132579
Chen T.-R.
Re-evaluation of HeLa, HeLa S3, and HEp-2 karyotypes.
Cytogenet. Cell Genet. 48:19-24(1988)


PubMed=3371749; DOI=10.1016/0090-8258(88)90029-7
Grenman S.E., Shapira A., Carey T.E.
In vitro response of cervical cancer cell lines CaSki, HeLa, and ME-180 to the antiestrogen tamoxifen.
Gynecol. Oncol. 30:228-238(1988)


PubMed=1522048; DOI=10.1007/BF02634140
Kataoka E., Honma M., Ohnishi K., Sofuni T., Mizusawa H.
Application of highly polymorphic DNA markers to the identification of HeLa cell sublines.
In Vitro Cell. Dev. Biol. Anim. 28:553-556(1992)


PubMed=8380785; DOI=10.1006/gyno.1993.1016
Iwasaka T., Oh-uchida M., Matsuo N., Yokoyama M., Fukuda K., Hara K., Fukuyama K., Hori K., Sugimori H.
Correlation between HPV positivity and state of the p53 gene in cervical carcinoma cell lines.
Gynecol. Oncol. 48:104-109(1993)


PubMed=9892199
Macville M.V.E., Schrock E., Padilla-Nash H.M., Keck C., Ghadimi B.M., Zimonjic D.B., Popescu N.C., Ried T.
Comprehensive and definitive molecular cytogenetic characterization of HeLa cells by spectral karyotyping.
Cancer Res. 59:141-150(1999)


PubMed=10423141; DOI=10.1099/0022-1317-80-7-1725
Meissner J.D.
Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines.
J. Gen. Virol. 80:1725-1733(1999)


PubMed=11416159; DOI=10.1073/pnas.121616198
Masters J.R.W., Thomson J.A., Daly-Burns B., Reid Y.A., Dirks W.G., Packer P., Toji L.H., Ohno T., Tanabe H., Arlett C.F., Kelland L.R., Harrison M., Virmani A.K., Ward T.H., Ayres K.L., Debenham P.G.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc. Natl. Acad. Sci. U.S.A. 98:8012-8017(2001)


PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)


PubMed=12001993; DOI=10.1038/nrc775
Masters J.R.W.
HeLa cells 50 years on: the good, the bad and the ugly.
Nat. Rev. Cancer 2:315-319(2002)


PubMed=12793746; DOI=10.1269/jrr.43.s133
Ohnishi T., Ohnishi K., Takahashi A., Taniguchi Y., Sato M., Nakano T., Nagaoka S.
Detection of DNA damage induced by space radiation in Mir and space shuttle.
J. Radiat. Res. 43:S133-S136(2002)


PubMed=12661003; DOI=10.1002/gcc.10196
Seitz S., Wassmuth P., Plaschke J., Schackert H.K., Karsten U., Santibanez-Koref M.F., Schlag P.M., Scherneck S.
Identification of microsatellite instability and mismatch repair gene mutations in breast cancer cell lines.
Genes Chromosomes Cancer 37:29-35(2003)


PubMed=15302935; DOI=10.1073/pnas.0404720101
Beausoleil S.A., Jedrychowski M.P., Schwartz D., Elias J.E., Villen J., Li J.-X., Cohn M.A., Cantley L.C., Gygi S.P.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004)


PubMed=15531914; DOI=10.1038/sj.onc.1208235
Baldus S.E., Schwarz E., Lohrey C., Zapatka M., Landsberg S., Hahn S.A., Schmidt D., Dienes H.-P., Schmiegel W.H., Schwarte-Waldhoff I.
Smad4 deficiency in cervical carcinoma cells.
Oncogene 24:810-819(2005)


PubMed=15901131; DOI=10.1016/j.prp.2005.01.002
Murai Y., Hayashi S., Takahashi H., Tsuneyama K., Takano Y.
Correlation between DNA alterations and p53 and p16 protein expression in cancer cell lines.
Pathol. Res. Pract. 201:109-115(2005)


PubMed=17311676; DOI=10.1186/1471-2164-8-53
Kloth J.N., Oosting J., van Wezel T., Szuhai K., Knijnenburg J., Gorter A., Kenter G.G., Fleuren G.J., Jordanova E.S.
Combined array-comparative genomic hybridization and single-nucleotide polymorphism-loss of heterozygosity analysis reveals complex genetic alterations in cervical cancer.
BMC Genomics 8:53.1-53.13(2007)


PubMed=19450234; DOI=10.2144/000113089
Rahbari R., Sheahan T., Modes V., Collier P., Macfarlane C.M., Badge R.M.
A novel L1 retrotransposon marker for HeLa cell line identification.
BioTechniques 46:277-284(2009)


PubMed=19722756; DOI=10.5858/133.9.1463
Lucey B.P., Nelson-Rees W.A., Hutchins G.M.
Henrietta Lacks, HeLa cells, and cell culture contamination.
Arch. Pathol. Lab. Med. 133:1463-1467(2009)



CLPUB00377

Skloot R.L.
The immortal life of Henrietta Lacks.
(In) ISBN 9781400052172; pp.1-400; Random House; New York (2010)




CLPUB00468

Javitt G.H.
Why not take all of me? Reflections on the immortal life of Henrietta Lacks and the status of participants in research using human specimens.
Minn. J. Law Sci. Technol. 11:713-755(2010)



PubMed=19941903; DOI=10.1016/j.jviromet.2009.11.022
Karger A., Bettin B., Lenk M., Mettenleiter T.C.
Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing.
J. Virol. Methods 164:116-121(2010)


PubMed=21269460; DOI=10.1186/1752-0509-5-17
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Burckstummer T., Bennett K.L., Superti-Furga G., Colinge J.
Initial characterization of the human central proteome.
BMC Syst. Biol. 5:17.1-17.13(2011)


PubMed=22068331; DOI=10.1038/msb.2011.81
Nagaraj N., Wisniewski J.R., Geiger T., Cox J., Kircher M., Kelso J., Paabo S., Mann M.
Deep proteome and transcriptome mapping of a human cancer cell line.
Mol. Syst. Biol. 7:548-548(2011)


PubMed=21937730; DOI=10.1074/mcp.M111.011429
Boisvert F.-M., Ahmad Y., Gierlinski M., Charriere F., Lamont D., Scott M., Barton G., Lamond A.I.
A quantitative spatial proteomics analysis of proteome turnover in human cells.
Mol. Cell. Proteomics 11:M111.011429-M111.011429(2012)


PubMed=22278370; DOI=10.1074/mcp.M111.014050
Geiger T., Wehner A., Schaab C., Cox J., Mann M.
Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins.
Mol. Cell. Proteomics 11:M111.014050-M111.014050(2012)


PubMed=22412903; DOI=10.1371/journal.pone.0032667
Vazquez-Mena O., Medina-Martinez I., Juarez-Torres E., Barron V., Espinosa A., Villegas-Sepulveda N., Gomez-Laguna L., Nieto-Martinez K., Orozco L., Roman-Bassaure E., Munoz Cortez S., Borges Ibanez M., Venegas-Vega C.A., Guardado-Estrada M., Rangel-Lopez A., Kofman S., Berumen J.
Amplified genes may be overexpressed, unchanged, or downregulated in cervical cancer cell lines.
PLoS ONE 7:E32667-E32667(2012)



CLPUB00507

Pultarova T.
HeLa cells: immortal space travellers.
Space Saf. Mag. 7:10-12(2013)



PubMed=23205564; DOI=10.1021/pr300859k
Malerod H., Graham R.L.J., Sweredoski M.J., Hess S.
Comprehensive profiling of N-linked glycosylation sites in HeLa cells using hydrazide enrichment.
J. Proteome Res. 12:248-259(2013)



公司简介 / Company profile
上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
           上海中乔新舟生物科技有限公司(官网:www.zqxzbio.com)成立于2011年,历经...
联系我们 / Contact
电 话:021-56760357;021-56760351
传 真:
邮 箱:sales@zqxzbio.com
邮 编:200439
地 址:上海市宝山区长江南路180号
Copyright © 2014 ZQXZBIO All rights reserved.
技术支持:中乔新舟沪ICP备14008091号