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人 IL-8酶联免疫试剂盒
英文名: Human IL-8 ELISA Kit
货号:EK0413
价格:¥4365.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定


人 IL-8酶联免疫试剂盒描述:


       Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.


       ScienCell's human IL-8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. Human IL-8-specific monoclonal antibodies are precoated onto 8 x 12 strips. The human specific detection monoclonal antibodies are biotinylated. The test samples and biotinylated detection antibodies are subsequently added to the wells and then washed with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The intensity of yellow is proportional to the amount of human IL-8 in the sample that is captured in the strips.


产品使用说明:仅供科研研究使用。

货号

EK0413

产地

美国

缩写

hIL-8-ELISA

规格

96 tests

用途

科研

保存

频繁使用储存在4°C,很少使用储存在-20°C.

运输

胶冰

参考文献

1. Modi, W. S.; Dean, M.; Seuanez, H. N.; Mukaida, N.; Matsushima, K.; O'Brien, S. J. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily. Hum. Genet. 84: 185-187, 1990. 
2. Bruun, J. M.; Pedersen, S. B.; Richelsen, B. Regulation of interleukin 8 production and gene expression in human adipose tissue in vitro. J. Clin. Endocr. Metab. 86: 1267-1273, 2001.


       1. Modi, W. S.; Dean, M.; Seuanez, H. N.; Mukaida, N.; Matsushima, K.; O'Brien, S. J. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily. Hum. Genet. 84: 185-187, 1990. 


       2. Bruun, J. M.; Pedersen, S. B.; Richelsen, B. Regulation of interleukin 8 production and gene expression in human adipose tissue in vitro. J. Clin. Endocr. Metab. 86: 1267-1273, 2001.

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