INS-1 cells (27) (a gift from C. Wollheim, Geneva, Switzerland) were routinely grown at 5% CO2-95% air at 37 C in RPMI-1640 containing 11.1 mM glucose and supplemented with 10% FBS, 1 mM pyruvate, 10 mM HEPES, 50 M 2-mercaptoethanol, 100 U penicillin/ml, and 100 g streptomycin/ml. In all the experiments described the RPMI-1640medium contained the supplements described above. Cells were passaged weekly after trypsin-EDTA detachment. All studies were performed on INS-1 passages between 70 and 84. 

论文标题： Palmatine ameliorates high fat diet induced impaired glucose tolerance
DOI： 10.1186/s40659-020-00308-0
发表时间： 2020-09-14
期刊： BIOLOGICAL RESEARCH
影响因子： 3.092
货号： ZQ0475
产品名称： INS-1 cells

原文链接： https://biolres.biomedcentral.com/articles/10.1186/s40659-020-00308-0

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