人星形胶质细胞描述：
在哺乳动物的大脑中，绝大部分细胞是星形胶质细胞。它们形态各异，并与神经元各个部分紧密相连，因此可能具有多种功能。星形胶质细胞为神经元提供结构，营养和代谢上的支持并调节突触活动。通过敲除或其它方式损坏星形胶质细胞可严重影响神经元的存活。此外，星形胶质细胞可提示许多神经疾病的病理过程。脑损伤后，通过神经突生长，突触可塑性和神经元再生的长期恢复过程受星形胶质细胞表面分子的表达和营养因子释放的影响。另外，星形胶质细胞自身存活或死亡可能影响最终的临床结果。星形胶质细胞在中枢神经系统功能的重要性越来越被重视，尤其是对于神经活动的调节。我们所获得的有关星形胶质细胞的知识主要来源于体外研究，星形胶质细胞的培养为探索细胞多样性提供了重要工具。
人星形胶质细胞(HA)提取于人脑(大脑皮层)，小神经胶质细胞(HAc)提取于人小脑组织。原代培养末期冻存，每管含有细胞数>5x106cells/ml，此细胞通过GFAP抗体荧光免疫方法验证，经测试不含有HIV-1、HBV、HCV、支原体、细菌、酵母和真菌。细胞可以达到10-15倍增。
配套培养基：星形胶质细胞培养基（AM.NO.1801)
产品使用说明：仅供科研研究使用
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Approximately how many days does it take to go from initiating the culture to 90% confluence? How many cells can I expect from a 90% confluent T75 flask?
Answers:
Posted by Manoj Sharma on Wednesday, April 20, 2016
Hi,
it takes about 3 to 4 days to go from initiating the culture to 90% confluence and you can expect upto 5 to 6 millions cells.
reply for further question
thank you
Question:2
Hi, I have been using humans astrocytes from your company. I was wondering about the % of astrocytes that are GFAP positive to begin with. Also, do each vial represent cells from different donors ?
Answers:
Posted by Manoj Sharma on Wednesday, April 20, 2016
Hi Priya,
More than 95% of astrocytes are GFAP positive and each vial represent from single donor.
please reply for further questions
Thank You
Question:3
Which genes could represent good housekeeping genes for RT-qPCR analysis of these cells? Any suggestions?
Answers:
Posted by Karishma Shah on Tuesday, January 12, 2016
Hello Laurent,
We recommend β-actin, GAPDH, LDHA, NONO, and PPIH to serve as housekeeping genes for astrocyte qRT-PCR. We also recommend using multiple housekeeping genes for data normalization.
In addition, in a qRT-PCR assay, we recommend using Genomic DNA (gDNA) Control (GDC) to detect possible gDNA contamination, and Positive PCR Control (PPC) to test whether samples contain inhibitors or other factors that
may negatively affect gene expression results.
ScienCell offers GeneQuery™ Human Astrocyte Cell Biology qPCR Array Kit for astrocyte qRT-PCR analysis. For more information, please go to:
http://www.sciencellonline.com/products-services/gene-analysis/genequery-human-astrocyte-cell-biology-qpcr-array-kit.html
http://www.sciencellonline.com/PS/GK009.pdf
Thanks,
SCIENCELL TEAM
Question:4
Are this cell lines？
Answers:
Posted by Manoj Sharma on Thursday, January 7, 2016
Hello,
They are primary cells, not immortalized cell lines. thanks for your interest
SCIENCELL
Question:
What is the recommended maximum number of passages that the primary human astrocytes can be grown to without losing phenotype? Is there a recommended freezing protocol?
Answers:
Posted by Manoj Sharma on Thursday, January 7, 2016
Hi Srabasti,
Human Astrocytes can be subcultured for 4-5 passages. However, for primary cells, the markers such as GFAP may be lost during each passage, even though they are still astrocytes. Therefore, we recommend that customers use the cells at early passage without expanding or passaging too much. We do not recommend re-freezing the cells for future use.
thanks for your interest
SCIENCELL TEAM
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