产品名称	Hacat人永生化表皮细胞
货号	ZQ0044
产品介绍	HaCaT细胞是一种人永生化表皮细胞系，源自一位62岁患有黑色素瘤男性的病灶外围正常皮肤组织。这种细胞系在体外培养后自然转化，获得了永生化特性，能够无限期地增殖而不会自然凋亡。HaCaT细胞保留了正常皮肤细胞的特性和功能，表达角蛋白、角化细胞交联外膜蛋白以及中间丝相关蛋白，这些蛋白在角质形成和皮肤细胞分化中起着重要的作用。HaCaT细胞因其在体外分化和增殖的高能力而被用于人表皮细胞角化的研究，并解决了培养寿命短和细胞系之间可能遇到的变异等问题。此外，它们也被广泛应用于皮肤生物学、免疫学、药物毒性研究、皮肤疾病研究、化妆品和药物测试等领域。 【注意事项】 该细胞传代时需要消化较长时间（5~15分钟），具体时间因胰酶浓度、效价、消化条件有所不同，请以细胞即将脱落为准。
种属	人
性别/年龄	男性/62岁
组织	正常表皮细胞 ,背部、皮肤、表皮
疾病	皮肤癌
生物安全等级	BSL -1
STR位点信息	Amelogenin：X； CSF1PO：9，11； D13S317：10，12； D16S539：9，12； D18S51：12； D19S433：13，14； D21S11：30.2； D2S1338：17，25； D3S1358：16； D5S818：12； D7S820：9，11； D8S1179：14； FGA：24； TH01：9.3； TPOX：11，12； vWA：16，17；
细胞类型	自发永生化细胞系
形态学	上皮样
生长方式	贴壁
倍增时间	大约28小时
培养基和添加剂	90%DMEM高糖（品牌：中乔新舟 货号：ZQ-100）+10%FBS（品牌：中乔新舟 货号：ZQ500-TS）+1%双抗（中乔新舟  货号：CSP006）
推荐完全培养基货号	ZM0044
培养条件	95%空气，5%二氧化碳；37℃
抗原表达/受体表达	***
基因表达	***
保藏机构	CLS；300493
供应限制	仅供科研使用

货号	ZQ0044
发货规格	活细胞：T25培养瓶*1瓶或者1ml 冻存管*1支（细胞量约为5 x 10^5 cells/vial）二选一
发货形式	活细胞：常温运输；冻存管：干冰运输
储存温度	活细胞：培养箱；冻存管：液氮罐
产地	中国
供应限制	仅供科研使用

论文标题： Oat β-glucan ameliorates epidermal barrier disruption by upregulating the expression of CaSR through dectin-1-mediated ERK and p38 signaling pathways
DOI： 10.1016/j.ijbiomac.2021.07.002
发表时间： 2021-07-05
期刊： INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
影响因子： 6.953
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://www.sciencedirect.com/science/article/pii/S0141813021014380

论文标题： Antioxidant-enriched autologous biogel promoted diabetic wound healing by remodeling inherent posttraumatic inflammatory patterning and restoring compromised microenvironment homeostasis
DOI： 10.1093/rb/rbac023
发表时间： 2022-04-08
期刊： Regenerative Biomaterials
影响因子： 6.353
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://academic.oup.com/rb/article-abstract/doi/10.1093/rb/rbac023/6575919

论文标题： Keratinocyte Growth Factor 2 Ameliorates UVB-Induced Skin Damage via Activating the AhR/Nrf2 Signaling Pathway
DOI： 10.3389/fphar.2021.655281
发表时间： 2021-06-07
期刊： Frontiers in Pharmacology
影响因子： 5.811
货号： ZQ0044
产品名称： HaCaT cells

原文链接： https://www.frontiersin.org/articles/10.3389/fphar.2021.655281/full

论文标题： Identification and validation of a ferroptosis-related gene signature for predicting survival in skin cutaneous melanoma
DOI： 10.1002/cam4.4706
发表时间： 2022-04-04
期刊： Cancer Medicine
影响因子： 4.452
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://onlinelibrary.wiley.com/doi/abs/10.1002/cam4.4706

论文标题： Quercetin promotes cutaneous wound healing in mice through Wnt/β-catenin signaling pathway
DOI： 10.1016/j.jep.2022.115066
发表时间： 2022-02-02
期刊： JOURNAL OF ETHNOPHARMACOLOGY
影响因子： 4.36
货号： ZQ0044
产品名称： HaCat cells
原文链接： https://www.sciencedirect.com/science/article/pii/S0378874122001003

论文标题： Carnosine Stimulates Macrophage-Mediated Clearance of Senescent Skin Cells Through Activation of the AKT2 Signaling Pathway by CD36 and RAGE
DOI： 10.3389/fphar.2020.593832
发表时间： 2020-12-16
期刊： Frontiers in Pharmacology
影响因子： 4.225
货号： ZQ0044
产品名称： Immortalized human keratinocyte (HaCaT)
原文链接： https://www.frontiersin.org/articles/10.3389/fphar.2020.593832/full

论文标题： Fucoidan from Undaria pinnatifida Ameliorates Epidermal Barrier Disruption via Keratinocyte Differentiation and CaSR Level Regulation
DOI： 10.3390/md17120660
发表时间： 2019-11-24
期刊： Marine Drugs
影响因子： 3.772
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://www.mdpi.com/580830

论文标题： Remodeling of dermal collagen in photoaged skin using low-dose 5-aminolevulinic acid photodynamic therapy occurs via the transforming growth factor-β pathway
DOI： 10.1002/jbio.201700357
发表时间： 2018-03-12
期刊： Journal of Biophotonics
影响因子： 3.768
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://onlinelibrary.wiley.com/doi/abs/10.1002/jbio.201700357

论文标题： MiR-508-3p promotes proliferation and inhibits apoptosis of middle ear cholesteatoma cells by targeting PTEN/PI3K/AKT pathway
DOI： 10.7150/ijms.60907
发表时间： 2021-07-11
期刊： International Journal of Medical Sciences
影响因子： 3.738
货号： ZQ0044
产品名称： human immortalized keratinocytes (HaCaT)
原文链接： https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8364443/

论文标题： Pseudoephedrine alleviates atopic dermatitis-like inflammatory responses in vivo and in vitro
DOI： 10.1016/j.lfs.2020.118139
发表时间： 2020-07-25
期刊： LIFE SCIENCES
影响因子： 3.647
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://www.sciencedirect.com/science/article/pii/S0024320520308900

论文标题： Resveratrol protects HaCaT cells from ultraviolet B-induced photoaging via upregulation of HSP27 and modulation of mitochondrial caspase-dependent apoptotic pathway
DOI： 10.1016/j.bbrc.2018.03.207
发表时间： 2018-03-31
期刊： BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子： 3.575
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://www.sciencedirect.com/science/article/pii/S0006291X18307368

论文标题： Circ_NCKAP1 promotes skin basal cell carcinoma progression by sponging the miR-148b-5p/HSP90 axis
DOI： 10.26355/eurrev_202109_26642
发表时间： 2021-09-01
期刊： European Review for Medical and Pharmacological Sciences
影响因子： 3.507
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://pubmed.ncbi.nlm.nih.gov/34533810/

论文标题： Identification and validation of potential novel biomarkers for oral squamous cell carcinoma
DOI： 10.1080/21655979.2021.1987089
发表时间： 2021-10-26
期刊： Bioengineered
影响因子： 3.269
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://www.tandfonline.com/doi/abs/10.1080/21655979.2021.1987089

论文标题： miR-205-5p inhibits psoriasis-associated proliferation and angiogenesis: Wnt/β-catenin and mitogen-activated protein kinase signaling pathway are involved
DOI： 10.1111/1346-8138.15370
发表时间： 2020-06-11
期刊： JOURNAL OF DERMATOLOGY
影响因子： 3.072
货号： ZQ0044
产品名称： human immortalized keratinocyte cell HaCaT
原文链接： https://onlinelibrary.wiley.com/doi/abs/10.1111/1346-8138.15370

论文标题： Schisandrin B protects human keratinocyte-derived HaCaT cells from tert-butyl hydroperoxide-induced oxidative damage through activating the Nrf2 signaling pathway
DOI： 10.3892/ijmm.2018.3901
发表时间： 2018-09-26
期刊： INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
影响因子： 2.784
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://www.spandidos-publications.com/10.3892/ijmm.2018.3901

论文标题： Protective effect of isopsoralen on UVB-induced injury in HaCaT cells via the ER and p38MAPK signaling pathways
DOI： 10.1111/jfbc.14163
发表时间： 2022-04-12
期刊： JOURNAL OF FOOD BIOCHEMISTRY
影响因子： 2.72
货号： ZQ0044
产品名称： HaCaT cells
原文链接： https://onlinelibrary.wiley.com/doi/abs/10.1111/jfbc.14163

论文标题： Elaeagnus L gum polysaccharides alleviate the impairment of barrier function in the dry skin model mice
DOI： 10.1111/jocd.13541
发表时间： 2020-10-24
期刊： Journal of Cosmetic Dermatology
影响因子： 1.621
货号： ZQ0044
产品名称： Human primary epidermal keratinocytes (HaCaT)
原文链接： https://onlinelibrary.wiley.com/doi/abs/10.1111/jocd.13541

论文标题： MiR-375-3p alleviates the severity of inflammation through targeting YAP1/LEKTI pathway in HaCaT cells
DOI： 10.1080/09168451.2020.1783196
发表时间： 2020-06-21
期刊： BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
影响因子： 1.516
货号： ZQ0044
产品名称： Human keratinocyte HaCaT cells

原文链接： https://www.tandfonline.com/doi/abs/10.1080/09168451.2020.1783196

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