产品名称 |
M-14人黑色素瘤细胞 |
货号 |
ZQ0045 |
产品介绍 |
M-14(也称为M14、UCLA-SO-M14、Melanoma14或M-14)是一种人黑色素瘤细胞系,最初是从一位33岁男性患者的无色素性黑色素瘤中分离建立的。这种细胞系在黑色素瘤的研究中具有重要价值,尤其是在药物筛选和肿瘤生物学特性研究方面。是黑色素瘤研究领域中的一个重要工具,它为科学家们提供了一个模拟人类黑色素瘤在体外条件下行为的模型。 |
种属 |
人 |
性别/年龄 |
男/33岁 |
组织 |
转移性;右臀,皮下组织 |
疾病 |
黑素瘤 |
细胞类型 |
肿瘤细胞 |
形态学 |
淋巴母细胞样 |
生长方式 |
半贴半悬 |
倍增时间 |
~26.3小时 |
培养基和添加剂 |
DMEM(高糖)基础培养基(中乔新舟 货号:ZQ-100)+10%FBS(品牌:中乔新舟 货号:ZQ500-A)+1%PS(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
|
生物安全等级 |
BSL - 1 |
STR位点信息 |
Amelogenin:X; |
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
*** |
基因表达 |
*** |
保藏机构 |
CLS;302163 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0045 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
1、PubMed=56444; DOI=10.1093/jnci/56.1.167
Golub S.H., Hanson D.C., Sulit H.L., Morton D.L., Pellegrino M.A., Ferrone S.
Comparison of histocompatibility antigens on cultured human tumor cells and fibroblasts by quantitative antibody absorption and sensitivity to cell-mediated cytotoxicity.
J. Natl. Cancer Inst. 56:167-170(1976)
2、PubMed=954005
Zielske J.V., Golub S.H.
Fetal calf serum-induced blastogenic and cytotoxic responses of human lymphocytes.
Cancer Res. 36:3842-3846(1976)
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Sulit H.L., Golub S.H., Irie R.F., Gupta R.K., Grooms G.A., Morton D.L.
Human tumor cells grown in fetal calf serum and human serum: influences on the tests for lymphocyte cytotoxicity, serum blocking and serum arming effects.
Int. J. Cancer 17:461-468(1976)
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Chee D.O., Boddie A.W. Jr., Roth J.A., Holmes E.C., Morton D.L.
Production of melanoma-associated antigen(s) by a defined malignant melanoma cell strain grown in chemically defined medium.
Cancer Res. 36:1503-1509(1976)
5、PubMed=204583; DOI=10.1002/ijc.2910210308
Saxton R.E., Irie R.F., Ferrone S., Pellegrino M.A., Morton D.L.
Establishment of paired tumor cells and autologous virus-transformed cell lines to define humoral immune responses in melanoma and sarcoma patients.
Int. J. Cancer 21:299-306(1978)
6、PubMed=6876798; DOI=10.1002/jso.2930230404
Chee D.O., Gupta R.K., Morton D.L.
Humoral response of melanoma patients to two different tumor-associated antigens.
J. Surg. Oncol. 23:228-235(1983)
7、PubMed=6470027; DOI=10.1007/BF00402467
Katano M., Saxton R.E., Cochran A.J., Irie R.F.
Establishment of an ascitic human melanoma cell line that metastasizes to lung and liver in nude mice.
J. Cancer Res. Clin. Oncol. 108:197-203(1984)
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Ferrini U., Falcioni R., Delpino A., Cavaliere R., Zupi G., Natali P.G.
Heat-shock proteins produced by two human melanoma cell lines: absence of correlation with thermosensitivity.
Int. J. Cancer 34:651-655(1984)
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Takahashi H., Adachi S., Yoshiya N., Suzuki T., Kanazawa K., Takeuchi S.
Expression of HLA-DR molecules in human gestational choriocarcinoma cell lines and malignant cell lines.
Placenta 8:293-298(1987)
10、PubMed=3674770
Greco C., Zupi G.
Biological features and in vitro chemosensitivity of a new model of human melanoma.
Anticancer Res. 7:839-844(1987)
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Wong J.H., Aguero B., Gupta R.K., Morton D.L.
Recovery of a cell surface fetal antigen from circulating immune complexes of melanoma patients.
Cancer Immunol. Immunother. 27:142-146(1988)
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Quax P.H.A., van Muijen G.N.P., Weening-Verhoeff E.J.D., Lund L.R., Dano K., Ruiter D.J., Verheijen J.H.
Metastatic behavior of human melanoma cell lines in nude mice correlates with urokinase-type plasminogen activator, its type-1 inhibitor, and urokinase-mediated matrix degradation.
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Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines.
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Antitumor effect of c-myc antisense phosphorothioate oligodeoxynucleotides on human melanoma cells in vitro and and in mice.
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Systematic variation in gene expression patterns in human cancer cell lines.
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Human melanoma cells secrete and respond to placenta growth factor and vascular endothelial growth factor.
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Mutations of the BRAF gene in human cancer.
Nature 417:949-954(2002)
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HLA class I and II genotype of the NCI-60 cell lines.
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Mutation analysis of 24 known cancer genes in the NCI-60 cell line set.
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DNA fingerprinting of the NCI-60 cell line panel.
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MDA-MB-435 and M14 cell lines: identical but not M14 melanoma?
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Systems-level modeling of cancer-fibroblast interaction.
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STR profiling of human cell lines: challenges and possible solutions to the growing problem.
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Expression and functional role of CRIPTO-1 in cutaneous melanoma.
Br. J. Cancer 105:1030-1038(2011)
28、PubMed=22068913; DOI=10.1073/pnas.1111840108
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Redefining the relevance of established cancer cell lines to the study of mechanisms of clinical anti-cancer drug resistance.
Proc. Natl. Acad. Sci. U.S.A. 108:18708-18713(2011)
29、PubMed=22347499; DOI=10.1371/journal.pone.0031628
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Mass homozygotes accumulation in the NCI-60 cancer cell lines as compared to HapMap trios, and relation to fragile site location.
PLoS ONE 7:E31628-E31628(2012)
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Identification of cancer cell-line origins using fluorescence image-based phenomic screening.
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The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology.
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Global proteome analysis of the NCI-60 cell line panel.
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34、PubMed=24279929; DOI=10.1186/2049-3002-1-20
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The metabolic demands of cancer cells are coupled to their size and protein synthesis rates.
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High resolution copy number variation data in the NCI-60 cancer cell lines from whole genome microarrays accessible through CellMiner.
PLoS ONE 9:E92047-E92047(2014)
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Iorio F., Knijnenburg T.A., Vis D.J., Bignell G.R., Menden M.P., Schubert M., Aben N., Goncalves E., Barthorpe S., Lightfoot H., Cokelaer T., Greninger P., van Dyk E., Chang H., de Silva H., Heyn H., Deng X.-M., Egan R.K., Liu Q.-S., Mironenko T., Mitropoulos X., Richardson L., Wang J.-H., Zhang T.-H., Moran S., Sayols S., Soleimani M., Tamborero D., Lopez-Bigas N., Ross-Macdonald P., Esteller M., Gray N.S., Haber D.A., Stratton M.R., Benes C.H., Wessels L.F.A., Saez-Rodriguez J., McDermott U., Garnett M.J.
A landscape of pharmacogenomic interactions in cancer.
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A map of mobile DNA insertions in the NCI-60 human cancer cell panel.
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Authentication of M14 melanoma cell line proves misidentification of MDA-MB-435 breast cancer cell line.
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Sini M.C., Doneddu V., Paliogiannis P., Casula M., Colombino M., Manca A., Botti G., Ascierto P.A., Lissia A., Cossu A., Palmieri G.
Genetic alterations in main candidate genes during melanoma progression.
Oncotarget 9:8531-8541(2018)
42、PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
43、PubMed=30971826; DOI=10.1038/s41586-019-1103-9
Behan F.M., Iorio F., Picco G., Goncalves E., Beaver C.M., Migliardi G., Santos R., Rao Y., Sassi F., Pinnelli M., Ansari R., Harper S., Jackson D.A., McRae R., Pooley R., Wilkinson P., van der Meer D.J., Dow D., Buser-Doepner C.A., Bertotti A., Trusolino L., Stronach E.A., Saez-Rodriguez J., Yusa K., Garnett M.J.
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Nature 568:511-516(2019)
产品名称 | 价格 | 指令 |
A375人恶性黑色素瘤细胞(STR鉴定)[套餐促销] | ¥1200.00 | 购物车 》 |
B16-F10小鼠黑色素瘤高转细胞(str鉴定)[套餐促销] | ¥1350.00 | 购物车 》 |
B16小鼠黑色素瘤细胞(种属鉴定) | ¥1000.00 | 购物车 》 |
支原体检测试剂盒 | ¥800.00 | 购物车 》 |
支原体清除试剂盒 | ¥600.00 | 购物车 》 |
国产转染试剂 | ¥800.00 | 购物车 》 |
慢病毒介导基因沉默或过表达 | ¥询价 | 购物车 》 |
细胞基因敲除 | ¥询价 | 购物车 》 |
DMEM(高糖)基础培养基 | ¥56.00 | 购物车 》 |
DMEM(高糖)完全培养基 | ¥350.00 | 购物车 》 |
优级胎牛血清 | ¥2580(开学促销) | 购物车 》 |
人源细胞STR鉴定服务 | ¥900.00 | 购物车 》 |
人黑色素瘤定量PCR芯片试剂盒 | ¥4320.00 | 购物车 》 |