产品名称 |
SH-SY5Y人神经母细胞瘤细胞 |
货号 |
ZQ0050 |
产品介绍 |
SH-SY5Y是一种人神经母细胞瘤细胞系,是神经母细胞瘤细胞系SK-N-SH的三次克隆(SK-N-SH-% 3E SH-SY-% 3E SH-SY5-% 3E SH-SY5Y)的亚系,最初于1970年从一位4岁女孩的骨髓中分离建立的,在形态、生理、生化功能上与人体细胞相似,可以转化为神经元样细胞,并表现出一系列具有高度指导意义的神经生物学特性。广泛应用于神经科学研究,包括神经退行性疾病(如帕金森病和阿尔茨海默病)、神经毒性、神经分化、基因功能研究以及信号传导途径研究等领域。它们可以经过特定的分化方案处理,分化为更接近于成熟神经元的细胞类型。是神经科学研究领域中的一个重要工具,它为科学家们提供了一个模拟人类神经母细胞瘤和神经元在体外条件下行为的模型。据报道,它们表现出中等水平的多巴胺β羟化酶活性。
注意事项: |
种属 |
人 |
性别/年龄 |
女/4岁 |
组织 |
转移性;骨髓 |
疾病 |
成神经细胞瘤 |
生物安全等级 |
BSL- 1 |
STR位点信息 |
Amelogenin: X
CSF1PO: 11 |
细胞类型 |
肿瘤细胞 |
形态学 |
上皮细胞样 |
生长方式 |
混合、贴壁和悬浮(该细胞培养时贴壁细胞与悬浮细胞同时存在,均为正常形态的活细胞.) |
倍增时间 |
> 55小时 |
培养基和添加剂 |
43.5%MEM(含NEAA)(货号:ZQ-300)+43.5%F12(中乔新舟 货号:ZQ-400) +10%FBS(货号:ZQ0500)+1%双抗(货号:CSP006)+1%Sodium Pyruvate(中乔新舟 货号:CSP003)+1%L-alanyl-L-glutamine(中乔新舟 货号:CSP004)+1% NEAA (中乔新舟 货号:CSP008) |
推荐完全培养基货号 |
|
培养条件 |
95%空气,5%二氧化碳;37℃ |
抗原表达/受体表达 |
Blood Type A; Rh+ |
基因表达 |
*** |
细胞来源 |
ATCC |
供应限制 |
仅供科研使用 |
货号 |
ZQ0050 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
论文标题: The Protective Effects of Osteocyte-Derived Extracellular Vesicles Against Alzheimer's Disease Diminished with Aging
DOI: 10.1002/advs.202105316
发表时间: 2022-05-04
期刊: Advanced Science
影响因子: 16.806
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202105316
论文标题: Inhibition of miR-331-3p and miR-9-5p ameliorates Alzheimer's disease by enhancing autophagy
DOI: 10.7150/thno.47408
发表时间: 2021-01-01
期刊: Theranostics
影响因子: 11.556
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797673/
论文标题: Movement Disorder and Neurotoxicity Induced by Chronic Exposure to Microcystin-LR in Mice
DOI: 10.1007/s12035-022-02919-y
发表时间: 2022-06-22
期刊: MOLECULAR NEUROBIOLOGY
影响因子: 5.682
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://link.springer.com/article/10.1007/s12035-022-02919-y
论文标题: Sulforaphane alleviates hyperalgesia and enhances analgesic potency of morphine in rats with cancer-induced bone pain
DOI: 10.1016/j.ejphar.2021.174412
发表时间: 2021-08-08
期刊: EUROPEAN JOURNAL OF PHARMACOLOGY
影响因子: 4.432
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0014299921005653
论文标题: The role of Fas-FasL-FADD signaling pathway in arsenic-mediated neuronal apoptosis in vivo and in vitro
DOI: 10.1016/j.toxlet.2021.11.012
发表时间: 2021-12-22
期刊: TOXICOLOGY LETTERS
影响因子: 4.374
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0378427421008936
论文标题: The abnormal phosphorylation of the Rac1, Lim-kinase 1, and Cofilin proteins in the pathogenesis of Hirschsprung’s disease
DOI: 10.1080/21655979.2022.2051882
发表时间: 2022-03-24
期刊: Bioengineered
影响因子: 3.269
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/21655979.2022.2051882
论文标题: Schizandrol A protects against Aβ 1–42 -induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons
DOI: 10.1007/s00210-019-01792-2
发表时间: 2020-01-04
期刊: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
影响因子: 3
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://link.springer.com/content/pdf/10.1007/s00210-019-01792-2.pdf
论文标题: Chlorogenic acid enhances autophagy by upregulating lysosomal function to protect against SH‑SY5Y cell injury induced by H<sub>2</sub>O<sub>2</sub>
DOI: 10.3892/etm.2021.9843
发表时间: 2021-02-26
期刊: Experimental and Therapeutic Medicine
影响因子: 2.447
货号: ZQ0050
产品名称: SH-SY5Y human neuroblastoma cells
原文链接: https://www.spandidos-publications.com/10.3892/etm.2021.9843
论文标题: Aberrant expression of LINC00346 regulates cell migration and proliferation via competitively binding to miRNA-148a-3p/Dnmt1 in Hirschsprung’s disease
DOI: 10.1007/s00383-022-05144-9
发表时间: 2022-07-14
期刊: PEDIATRIC SURGERY INTERNATIONAL
影响因子: 2.003
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://link.springer.com/article/10.1007/s00383-022-05144-9
论文标题: The antidepressant effects of apigenin are associated with the promotion of autophagy via the mTOR/AMPK/ULK1 pathway
DOI: 10.3892/mmr.2019.10491
发表时间: 2019-07-12
期刊: Molecular Medicine Reports
影响因子: 1.851
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.spandidos-publications.com/10.3892/mmr.2019.10491?text=fulltext
论文标题: Downregulation of miR-140-5p affects the pathogenesis of HSCR by targeting EGR2
DOI: 10.1007/s00383-020-04686-0
发表时间: 2020-06-07
期刊: PEDIATRIC SURGERY INTERNATIONAL
影响因子: 1.668
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://link.springer.com/content/pdf/10.1007/s00383-020-04686-0.pdf
PubMed=4748425
Biedler J.L., Helson L., Spengler B.A.
Morphology and growth, tumorigenicity, and cytogenetics of human neuroblastoma cells in continuous culture.
Cancer Res. 33:2643-2652(1973)
PubMed=6137586; DOI=10.1093/jnci/71.4.741
Ross R.A., Spengler B.A., Biedler J.L.
Coordinate morphological and biochemical interconversion of human neuroblastoma cells.
J. Natl. Cancer Inst. 71:741-747(1983)
PubMed=3968181; DOI=10.1083/jcb.100.2.588; PMCID=PMC2113449
Meyers M.B., Spengler B.A., Chang T.-D., Melera P.W., Biedler J.L.
Gene amplification-associated cytogenetic aberrations and protein changes in vincristine-resistant Chinese hamster, mouse, and human cells.
J. Cell Biol. 100:588-597(1985)
PubMed=2846152
Preis P.N., Saya H., Nadasdi L., Hochhaus G., Levin V.A., Sadee W.
Neuronal cell differentiation of human neuroblastoma cells by retinoic acid plus herbimycin A.
Cancer Res. 48:6530-6534(1988)
PubMed=2535691
Ciccarone V.C., Spengler B.A., Meyers M.B., Biedler J.L., Ross R.A.
Phenotypic diversification in human neuroblastoma cells: expression of distinct neural crest lineages.
Cancer Res. 49:219-225(1989)
PubMed=1354203; DOI=10.1002/ijc.2910520116
Gross N., Favre S., Beck D., Meyer M.
Differentiation-related expression of adhesion molecules and receptors on human neuroblastoma tissues, cell lines and variants.
Int. J. Cancer 52:85-91(1992)
PubMed=7687927
Xu Y.-Y., Wands J.R., de la Monte S.M.
Characterization of thread proteins expressed in neuroectodermal tumors.
Cancer Res. 53:3823-3829(1993)
PubMed=10630978; DOI=10.1667/0033-7587(2000)153[0062:FORIMI]2.0.CO;2
Akudugu J.M., Slabbert J.P., Serafin A.M., Bohm L.
Frequency of radiation-induced micronuclei in neuronal cells does not correlate with clonogenic survival.
Radiat. Res. 153:62-67(2000)
PubMed=11668190; DOI=10.1177/002215540104901105
Quentmeier H., Osborn M., Reinhardt J., Zaborski M., Drexler H.G.
Immunocytochemical analysis of cell lines derived from solid tumors.
J. Histochem. Cytochem. 49:1369-1378(2001)
PubMed=16120276; DOI=10.1016/S1567-7249(01)00007-1
Scheffler N.K., Miller S.W., Carroll A.K., Anderson C.M., Davis R.E., Ghosh S.S., Gibson B.W.
Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from an SH-SY5Y neuroblastoma cell line.
Mitochondrion 1:161-179(2001)
PubMed=12505268; DOI=10.1016/S0165-4608(02)00523-x
Spengler B.A., Biedler J.L., Ross R.A.
A corrected karyotype for the SH-SY5Y human neuroblastoma cell line.
Cancer Genet. Cytogenet. 138:177-178(2002)
PubMed=14676279; DOI=10.1242/jcs.00834
Goldschneider D., Blanc E., Raguenez G., Barrois M., Legrand A., Le Roux G., Haddada H., Benard J., Douc-Rasy S.
Differential response of p53 target genes to p73 overexpression in SH-SY5Y neuroblastoma cell line.
J. Cell Sci. 117:293-301(2004)
PubMed=15150091; DOI=10.1158/0008-5472.CAN-03-0809
Schaefer K.-L., Brachwitz K., Wai D.H., Braun Y., Diallo R., Korsching E., Eisenacher M., Voss R., van Valen F., Baer C., Selle B., Spahn L., Liao S.-K., Lee K.A.W., Hogendoorn P.C.W., Reifenberger G., Gabbert H.E., Poremba C.
Expression profiling of t(12;22) positive clear cell sarcoma of soft tissue cell lines reveals characteristic up-regulation of potential new marker genes including ERBB3.
Cancer Res. 64:3395-3405(2004)
PubMed=15720811; DOI=10.1593/neo.04310; PMCID=PMC1531688
Walton J.D., Kattan D.R., Thomas S.K., Spengler B.A., Guo H.-F., Biedler J.L., Cheung N.-K.V., Ross R.A.
Characteristics of stem cells from human neuroblastoma cell lines and in tumors.
Neoplasia 6:838-845(2004)
PubMed=16822308; DOI=10.1186/1471-2407-6-177; PMCID=PMC1533846
Dam V., Morgan B.T., Mazanek P., Hogarty M.D.
Mutations in PIK3CA are infrequent in neuroblastoma.
BMC Cancer 6:177.1-177.10(2006)
PubMed=17506115; DOI=10.1002/nbm.1181
Peet A.C., McConville C.M., Wilson M.P., Levine B.A., Reed M., Dyer S.A., Edwards E.C., Strachan M.C., McMullan D.J., Wilkes T.M., Grundy R.G.
1H MRS identifies specific metabolite profiles associated with MYCN-amplified and non-amplified tumour subtypes of neuroblastoma cell lines.
NMR Biomed. 20:692-700(2007)
PubMed=18957096; DOI=10.1186/1756-0500-1-95; PMCID=PMC2615028
Nishida Y., Adati N., Ozawa R., Maeda A., Sakaki Y., Takeda T.
Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y.
BMC Res. Notes 1:95.1-95.11(2008)
PubMed=20655465; DOI=10.1016/j.cell.2010.06.004; PMCID=PMC2913027
Holzel M., Huang S.-D., Koster J., Ora I., Lakeman A., Caron H.N., Nijkamp W., Xie J., Callens T., Asgharzadeh S., Seeger R.C., Messiaen L.M., Versteeg R., Bernards R.
NF1 is a tumor suppressor in neuroblastoma that determines retinoic acid response and disease outcome.
Cell 142:218-229(2010)
PubMed=22213050; DOI=10.1002/ijc.27415; PMCID=PMC3757132
Gawecka J.E., Geerts D., Koster J., Caliva M.J., Sulzmaier F.J., Opoku-Ansah J., Wada R.K., Bachmann A.S., Ramos J.W.
PEA15 impairs cell migration and correlates with clinical features predicting good prognosis in neuroblastoma.
Int. J. Cancer 131:1556-1568(2012)
PubMed=22460905; DOI=10.1038/nature11003; PMCID=PMC3320027
Barretina J.G., Caponigro G., Stransky N., Venkatesan K., Margolin A.A., Kim S., Wilson C.J., Lehar J., Kryukov G.V., Sonkin D., Reddy A., Liu M., Murray L., Berger M.F., Monahan J.E., Morais P., Meltzer J., Korejwa A., Jane-Valbuena J., Mapa F.A., Thibault J., Bric-Furlong E., Raman P., Shipway A., Engels I.H., Cheng J., Yu G.-Y.K., Yu J.-J., Aspesi P. Jr., de Silva M., Jagtap K., Jones M.D., Wang L., Hatton C., Palescandolo E., Gupta S., Mahan S., Sougnez C., Onofrio R.C., Liefeld T., MacConaill L.E., Winckler W., Reich M., Li N.-X., Mesirov J.P., Gabriel S.B., Getz G., Ardlie K., Chan V., Myer V.E., Weber B.L., Porter J., Warmuth M., Finan P., Harris J.L., Meyerson M.L., Golub T.R., Morrissey M.P., Sellers W.R., Schlegel R., Garraway L.A.
The Cancer Cell Line Encyclopedia enables predictive modelling of anticancer drug sensitivity.
Nature 483:603-607(2012)
PubMed=23421552; DOI=10.1089/ast.2012.0822
Zhang Y.-Q., Wang H.-B., Lai C.-J., Wang L., Deng Y.-L.
Comparative proteomic analysis of human SH-SY5Y neuroblastoma cells under simulated microgravity.
Astrobiology 13:143-150(2013)
PubMed=24466371; DOI=10.1593/tlo.13544; PMCID=PMC3890703
Loschmann N., Michaelis M., Rothweiler F., Zehner R., Cinatl J., Voges Y., Sharifi M., Riecken K., Meyer J., von Deimling A., Fichtner I., Ghafourian T., Westermann F., Cinatl J. Jr.
Testing of SNS-032 in a panel of human neuroblastoma cell lines with acquired resistance to a broad range of drugs.
Transl. Oncol. 6:685-696(2013)
PubMed=25182563; DOI=10.1007/s13577-014-0096-9
Jiang T., Wang H.-L.
Identification of cross-contamination in SH-SY5Y cell line.
Hum. Cell 27:176-178(2014)
PubMed=25730103; DOI=10.1021/pr5011718; PMCID=PMC4516140
Hu Y.-L., Mayampurath A., Khan S., Cohen J.K., Mechref Y., Volchenboum S.L.
N-linked glycan profiling in neuroblastoma cell lines.
J. Proteome Res. 14:2074-2081(2015)
PubMed=25884760; DOI=10.1371/journal.pcbi.1004130; PMCID=PMC4401789
Palacios-Moreno J., Foltz L., Guo A.-L., Stokes M.P., Kuehn E.D., George L., Comb M.J., Grimes M.L.
Neuroblastoma tyrosine kinase signaling networks involve FYN and LYN in endosomes and lipid rafts.
PLoS Comput. Biol. 11:e1004130.1-e1004130.33(2015)
PubMed=26342562; DOI=10.1016/j.scr.2015.08.008; PMCID=PMC4601571
Ross R.A., Walton J.D., Han D., Guo H.-F., Cheung N.-K.V.
A distinct gene expression signature characterizes human neuroblastoma cancer stem cells.
Stem Cell Res. 15:419-426(2015)
PubMed=26972028; DOI=10.1016/j.jprot.2016.03.008
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Identification of glycosylphosphatidylinositol-anchored proteins and omega-sites using TiO2-based affinity purification followed by hydrogen fluoride treatment.
J. Proteomics 139:77-83(2016)
PubMed=27141528; DOI=10.1016/j.dib.2016.04.001; PMCID=PMC4838930
Masuishi Y., Kimura Y., Arakawa N., Hirano H.
Data for identification of GPI-anchored peptides and omega-sites in cancer cell lines.
Data Brief 7:1302-1305(2016)
PubMed=28118852; DOI=10.1186/s13024-017-0149-0; PMCID=PMC5259880
Xicoy H., Wieringa B., Martens G.J.M.
The SH-SY5Y cell line in Parkinson's disease research: a systematic review.
Mol. Neurodegener. 12:10.1-10.11(2017)
PubMed=28350380; DOI=10.1038/sdata.2017.33; PMCID=PMC5369315
Harenza J.L., Diamond M.A., Adams R.N., Song M.M., Davidson H.L., Hart L.S., Dent M.H., Fortina P., Reynolds C.P., Maris J.M.
Transcriptomic profiling of 39 commonly-used neuroblastoma cell lines.
Sci. Data 4:170033-170033(2017)
PubMed=28601559; DOI=10.1016/j.cels.2017.05.009; PMCID=PMC5493283
Bekker-Jensen D.B., Kelstrup C.D., Batth T.S., Larsen S.C., Haldrup C., Bramsen J.B., Sorensen K.D., Hoyer S., Orntoft T.F., Lindbjerg Andersen C., Nielsen M.L., Olsen J.V.
An optimized shotgun strategy for the rapid generation of comprehensive human proteomes.
Cell Syst. 4:587-599.e4(2017)
PubMed=29468137; DOI=10.5501/wjv.v7.i1.10; PMCID=PMC5807893
Himmelsbach K., Hildt E.
Identification of various cell culture models for the study of Zika virus.
World J. Virol. 7:10-20(2018)
PubMed=30894373; DOI=10.1158/0008-5472.CAN-18-2747; PMCID=PMC6445675
Dutil J., Chen Z.-H., Monteiro A.N.A., Teer J.K., Eschrich S.A.
An interactive resource to probe genetic diversity and estimated ancestry in cancer cell lines.
Cancer Res. 79:1263-1273(2019)
PubMed=31068700; DOI=10.1038/s41586-019-1186-3; PMCID=PMC6697103
Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. 3rd, Barretina J.G., Gelfand E.T., Bielski C.M., Li H.-X., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y.-L., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.
Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)
产品名称 | 价格 | 指令 |
SK-N-AS人神经母细胞瘤细胞(STR鉴定) | ¥1500.00 | 购物车 》 |
IMR-32人神经母细胞瘤细胞 (STR鉴定) | ¥1350.00 | 购物车 》 |
SK-N-BE(2)人神经母细胞瘤细胞 (STR鉴定) | ¥1350.00 | 购物车 》 |
SK-N-SH人神经母细胞瘤细胞 | ¥1350.00 | 购物车 》 |
支原体检测试剂盒 | ¥800.00 | 购物车 》 |
支原体清除试剂盒 | ¥600.00 | 购物车 》 |
国产转染试剂 | ¥800.00 | 购物车 》 |
优级胎牛血清 | ¥2580(开学促销) | 购物车 》 |
F-12基础培养基 | ¥96.00 | 购物车 》 |
MEM α 基础培养基(含核苷和脱氧核苷) | ¥76.00 | 购物车 》 |
MEM/F-12完全培养基 | ¥850.00 | 购物车 》 |