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SH-SY5Y人神经母细胞瘤细胞(STR鉴定)
英文名:SH-SY5Y
货号:ZQ0050
价格:¥1600.00
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推荐组合

SH-SY5Y人神经母细胞瘤细胞(STR鉴定)

¥1600.00
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SH-SY5Y [SHSY-5Y]人神经母细胞瘤细胞专用培养基

¥650.00

配套完培,组合使用,省时省力!

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细胞套餐惊爆价

¥2250 ¥2250.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

SH-SY5Y人神经母细胞瘤细胞

货号

ZQ0050

产品介绍

SH-SY5Y是一种人神经母细胞瘤细胞系,是神经母细胞瘤细胞系SK-N-SH的三次克隆(SK-N-SH-% 3E SH-SY-% 3E SH-SY5-% 3E SH-SY5Y)的亚系,最初于1970年从一位4岁女孩的骨髓中分离建立的,在形态、生理、生化功能上与人体细胞相似,可以转化为神经元样细胞,并表现出一系列具有高度指导意义的神经生物学特性。广泛应用于神经科学研究,包括神经退行性疾病(如帕金森病和阿尔茨海默病)、神经毒性、神经分化、基因功能研究以及信号传导途径研究等领域。它们可以经过特定的分化方案处理,分化为更接近于成熟神经元的细胞类型。是神经科学研究领域中的一个重要工具,它为科学家们提供了一个模拟人类神经母细胞瘤和神经元在体外条件下行为的模型。据报道,它们表现出中等水平的多巴胺β羟化酶活性。

注意事项:
1. 该细胞在培养时以贴壁细胞和悬浮细胞混合形态,这些细胞均为活细胞。如果在培养过程中发现绝大部分细胞呈现贴壁状态,很少有悬浮的活细胞,这是正常情况。我们建议用户按照贴壁细胞的方式进行消化和培养。
2. 如果培养过程中发现悬浮的细胞数量比较多,或者细胞虽然为贴壁状态但容易聚团且生长缓慢,则需要在换液和传代时通过离心收集这些悬浮细胞,并且尽快更换高质量的血清以促进细胞贴壁和生长。
3. 该细胞对血清质量较为敏感,我司建议用户使用高质量胎牛血清或订购该细胞完全培养液。

种属

性别/年龄

/4岁

组织

转移性;骨髓

疾病

成神经细胞瘤

生物安全等级

BSL- 1

STR位点信息

Amelogenin: X

CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
TH01: 7,10
TPOX: 8,11
vWA: 14,18
D3S1358: 15,16
D21S11: 31,31.2
D18S51: 13,16
Penta_E: 7,11
Penta_D: 10,12
D8S1179: 15
FGA: 23.2,24
D19S433: 13,14
D2S1338: 17,19

细胞类型

肿瘤细胞

形态学

上皮细胞样

生长方式

混合、贴壁和悬浮(该细胞培养时贴壁细胞与悬浮细胞同时存在,均为正常形态的活细胞.)

倍增时间

> 55小时 

培养基和添加剂

43.5%MEM(含NEAA)(货号:ZQ-300+43.5%F12(中乔新舟  货号:ZQ-400  +10%FBS(货号:ZQ0500+1%双抗(货号:CSP006+1%Sodium Pyruvate(中乔新舟  货号:CSP003+1%L-alanyl-L-glutamine(中乔新舟   货号:CSP004+1% NEAA  (中乔新舟    货号:CSP008)

推荐完全培养基货号

ZM0050

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

Blood Type A; Rh+

基因表达

*** 

细胞来源

ATCC

供应限制

仅供科研使用

货号

ZQ0050

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


论文标题: The Protective Effects of Osteocyte-Derived Extracellular Vesicles Against Alzheimer's Disease Diminished with Aging

DOI: 10.1002/advs.202105316
发表时间: 2022-05-04
期刊: Advanced Science
影响因子: 16.806
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/advs.202105316


论文标题: Inhibition of miR-331-3p and miR-9-5p ameliorates Alzheimer's disease by enhancing autophagy
DOI: 10.7150/thno.47408
发表时间: 2021-01-01
期刊: Theranostics
影响因子: 11.556
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7797673/


论文标题: Movement Disorder and Neurotoxicity Induced by Chronic Exposure to Microcystin-LR in Mice
DOI: 10.1007/s12035-022-02919-y
发表时间: 2022-06-22
期刊: MOLECULAR NEUROBIOLOGY
影响因子: 5.682
货号: ZQ0050
产品名称: SH-SY5Y cells

原文链接: https://link.springer.com/article/10.1007/s12035-022-02919-y


论文标题: Sulforaphane alleviates hyperalgesia and enhances analgesic potency of morphine in rats with cancer-induced bone pain
DOI: 10.1016/j.ejphar.2021.174412
发表时间: 2021-08-08
期刊: EUROPEAN JOURNAL OF PHARMACOLOGY
影响因子: 4.432
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0014299921005653


论文标题: The role of Fas-FasL-FADD signaling pathway in arsenic-mediated neuronal apoptosis in vivo and in vitro
DOI: 10.1016/j.toxlet.2021.11.012
发表时间: 2021-12-22
期刊: TOXICOLOGY LETTERS
影响因子: 4.374
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.sciencedirect.com/science/article/pii/S0378427421008936


论文标题: The abnormal phosphorylation of the Rac1, Lim-kinase 1, and Cofilin proteins in the pathogenesis of Hirschsprung’s disease
DOI: 10.1080/21655979.2022.2051882
发表时间: 2022-03-24
期刊: Bioengineered
影响因子: 3.269
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.tandfonline.com/doi/abs/10.1080/21655979.2022.2051882


论文标题: Schizandrol A protects against Aβ 1–42 -induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons
DOI: 10.1007/s00210-019-01792-2
发表时间: 2020-01-04
期刊: NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
影响因子: 3
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://link.springer.com/content/pdf/10.1007/s00210-019-01792-2.pdf


论文标题: Chlorogenic acid enhances autophagy by upregulating lysosomal function to protect against SH‑SY5Y cell injury induced by H<sub>2</sub>O<sub>2</sub>
DOI: 10.3892/etm.2021.9843
发表时间: 2021-02-26
期刊: Experimental and Therapeutic Medicine
影响因子: 2.447
货号: ZQ0050
产品名称: SH-SY5Y human neuroblastoma cells
原文链接: https://www.spandidos-publications.com/10.3892/etm.2021.9843


论文标题: Aberrant expression of LINC00346 regulates cell migration and proliferation via competitively binding to miRNA-148a-3p/Dnmt1 in Hirschsprung’s disease
DOI: 10.1007/s00383-022-05144-9
发表时间: 2022-07-14
期刊: PEDIATRIC SURGERY INTERNATIONAL
影响因子: 2.003
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://link.springer.com/article/10.1007/s00383-022-05144-9


论文标题: The antidepressant effects of apigenin are associated with the promotion of autophagy via the mTOR/AMPK/ULK1 pathway
DOI: 10.3892/mmr.2019.10491
发表时间: 2019-07-12
期刊: Molecular Medicine Reports
影响因子: 1.851
货号: ZQ0050
产品名称: SH-SY5Y cells
原文链接: https://www.spandidos-publications.com/10.3892/mmr.2019.10491?text=fulltext


论文标题: Downregulation of miR-140-5p affects the pathogenesis of HSCR by targeting EGR2
DOI: 10.1007/s00383-020-04686-0
发表时间: 2020-06-07
期刊: PEDIATRIC SURGERY INTERNATIONAL
影响因子: 1.668
货号: ZQ0050
产品名称: SH-SY5Y cells

原文链接: https://link.springer.com/content/pdf/10.1007/s00383-020-04686-0.pdf



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PubMed=18957096; DOI=10.1186/1756-0500-1-95; PMCID=PMC2615028
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Identification and classification of genes regulated by phosphatidylinositol 3-kinase- and TRKB-mediated signalling pathways during neuronal differentiation in two subtypes of the human neuroblastoma cell line SH-SY5Y.
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Next-generation characterization of the Cancer Cell Line Encyclopedia.
Nature 569:503-508(2019)



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