名称 |
C2C12小鼠胚胎肌母细胞 |
货号 |
ZQ0092 |
产品介绍 |
该细胞株是YaffeD,SaxelO建立的小鼠成肌细胞系的亚株。该细胞分化较快,可形成能收缩的微管,产生特异的肌肉蛋白。在骨形态形成蛋白(BMP-2)的作用下,该细胞可由成肌细胞分化为成骨细胞。检测发现该细胞鼠痘病毒阴性。 注意事项:C2C12细胞生长较快,密度达到80%即可传代。培养时细胞密度不能过高(细胞密度控制在 5000 – 15000 活细胞/平方厘米), 并应及时更换培养液,否则容易分化。 收到邮寄活细胞的,收集上清离心后收集脱落的悬浮细胞和贴壁细胞一起建议T25培养瓶1:2传代培养,参考传代比例根据细胞数量决定。传代周期2-3天,隔天换液。 |
种属 |
小鼠 |
组织来源 |
骨骼肌组织 |
形态学 |
成肌细胞样 |
细胞类型 |
自然永生细胞系 |
倍增时间 |
~18 .4-20 hours |
生长方式 |
贴壁 |
培养基和添加剂 |
DMEM高糖(品牌:中乔新舟 货号:ZQ-100)+10%FBS(血清灭活)(中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟 货号:CSP006) |
推荐完全培养基货号 |
ZM0092 |
生物安全等级 |
BSL-1 |
培养条件 |
95%空气,5%二氧化碳;37℃ |
保藏机构 |
ATCC; CRL-1772 BCRC; 60083 BCRJ; 0058 CCTCC; GDC0175 CLS; 400476 DSMZ; ACC-565 ECACC; 91031101 |
供应限制 |
仅供科研使用 |
货号 |
ZQ0092 |
发货规格 |
活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一 |
发货形式 |
活细胞:常温运输;冻存管:干冰运输 |
储存温度 |
活细胞:培养箱;冻存管:液氮罐 |
产地 |
中国 |
供应限制 |
仅供科研使用 |
原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8544334/
2.论文标题: Programmed cell death 5 improves skeletal muscle insulin resistance by inhibiting IRS-1 ubiquitination through stabilization of MDM2
DOI: 10.1016/j.lfs.2021.119918
发表时间: 2021-09-01
期刊: LIFE SCIENCES
影响因子: 5.037
货号: ZQ0092
产品名称: C2C12 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S002432052100905X
3.论文标题: Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro
DOI: 10.1002/mnfr.201600673
发表时间: 2017-04-21
期刊: MOLECULAR NUTRITION & FOOD RESEARCH
影响因子: 4.323
货号: ZQ0092
产品名称: C2C12 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.201600673
4.论文标题: Identification of mangiferin as a potential Glucokinase activator by structure-based virtual ligand screening
DOI: 10.1038/srep44681
发表时间: 2017-03-20
期刊: Scientific Reports
影响因子: 4.259
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.nature.com/articles/srep44681
5.论文标题: Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis
DOI: 10.1016/j.yexcr.2020.111890
发表时间: 2020-02-05
期刊: EXPERIMENTAL CELL RESEARCH
影响因子: 3.905
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0014482720300896
6.论文标题: Type 2 diabetes-induced overactivation of P300 contributes to skeletal muscle atrophy by inhibiting autophagic flux
DOI: 10.1016/j.lfs.2020.118243
发表时间: 2020-08-10
期刊: LIFE SCIENCES
影响因子: 3.647
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0024320520309954
7.论文标题: Impaired autophagic flux contributes to muscle atrophy in obesity by affecting muscle degradation and regeneration
DOI: 10.1016/j.bbrc.2020.02.110
发表时间: 2020-02-24
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子: 2.985
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0006291X20303843
8.论文标题: Effects of acute hypoxia exposure with different durations on activation of Nrf2-ARE pathway in mouse skeletal muscle
DOI: 10.1371/journal.pone.0208474
发表时间: 2018-12-04
期刊: PLoS One
影响因子: 2.766
货号: ZQ0092
产品名称: C2C12 cells
原文链接: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208474
9.论文标题: Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes
DOI: 10.1016/j.acthis.2019.04.001
发表时间: 2019-04-30
期刊: ACTA HISTOCHEMICA
影响因子: 2.479
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0065128118304355
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产品名称 | 价格 | 指令 |
So18小鼠骨骼肌成肌细胞(种属鉴定) | ¥1700.00 | 购物车 》 |
L6大鼠成肌细胞(种属鉴定) | ¥1350.00 | 购物车 》 |
支原体检测试剂盒 | ¥800.00 | 购物车 》 |
支原体清除试剂盒 | ¥600.00 | 购物车 》 |
国产转染试剂 | ¥800.00 | 购物车 》 |
慢病毒介导基因沉默或过表达 | ¥询价 | 购物车 》 |
荧光素酶标记 | ¥询价 | 购物车 》 |
RPMI-1640基础培养基 | ¥56.00 | 购物车 》 |
RPMI-1640完全培养基 | ¥350.00 | 购物车 》 |
优级胎牛血清 | ¥2580(开学促销) | 购物车 》 |
人源细胞STR鉴定服务 | ¥900.00 | 购物车 》 |