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C2C12小鼠胚胎肌母细胞(种属鉴定)(建议发冻存管)
英文名:C2C12
货号:ZQ0092
价格:¥1350.00
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推荐组合

C2C12小鼠胚胎肌母细胞(种属鉴定)(建议发冻存管)

¥1350.00
+

C2C12小鼠成肌细胞专用培养基

¥500.00

配套完培,组合使用,省时省力!

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细胞套餐惊爆价

¥1850
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

名称

C2C12小鼠胚胎肌母细胞

货号

ZQ0092

产品介绍

该细胞株是YaffeD,SaxelO建立的小鼠成肌细胞系的亚株。该细胞分化较快,可形成能收缩的微管,产生特异的肌肉蛋白。在骨形态形成蛋白(BMP-2)的作用下,该细胞可由成肌细胞分化为成骨细胞。检测发现该细胞鼠痘病毒阴性。 

注意事项:C2C12细胞生长较快,密度达到80%即可传代。培养时细胞密度不能过高(细胞密度控制在 5000 – 15000 活细胞/平方厘米), 并应及时更换培养液,否则容易分化。

收到邮寄活细胞的,收集上清离心后收集脱落的悬浮细胞和贴壁细胞一起建议T25培养瓶1:2传代培养,参考传代比例根据细胞数量决定。传代周期2-3天,隔天换液。

种属

小鼠

组织来源

骨骼肌组织

形态学

成肌细胞样

细胞类型

自然永生细胞系

倍增时间

 ~18 .4-20 hours

生长方式

贴壁

培养基和添加剂

DMEM高糖(品牌:中乔新舟 货号:ZQ-100+10%FBS(血清灭活)(中乔新舟  货号:ZQ500-A+1%双抗(中乔新舟   货号:CSP006

推荐完全培养基货号

ZM0092

生物安全等级

BSL-1

培养条件

95%空气,5%二氧化碳;37℃

保藏机构

ATCC; CRL-1772

BCRC; 60083

BCRJ; 0058

CCTCC; GDC0175

CLS; 400476

DSMZ; ACC-565

ECACC; 91031101

供应限制

仅供科研使用

货号

ZQ0092

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


1.论文标题: Vitellogenin 2 promotes muscle development and stimulates the browning of white fat
DOI: 10.18632/aging.203590
发表时间: 2021-10-05
期刊: Aging-US
影响因子: 5.682
货号: ZQ0092
产品名称: C2C12 cells

原文链接: https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8544334/


2.论文标题: Programmed cell death 5 improves skeletal muscle insulin resistance by inhibiting IRS-1 ubiquitination through stabilization of MDM2
DOI: 10.1016/j.lfs.2021.119918
发表时间: 2021-09-01
期刊: LIFE SCIENCES
影响因子: 5.037
货号: ZQ0092
产品名称: C2C12 cells
原文链接: https://www.sciencedirect.com/science/article/pii/S002432052100905X


3.论文标题: Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro
DOI: 10.1002/mnfr.201600673
发表时间: 2017-04-21
期刊: MOLECULAR NUTRITION & FOOD RESEARCH
影响因子: 4.323
货号: ZQ0092
产品名称: C2C12 cells
原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.201600673


4.论文标题: Identification of mangiferin as a potential Glucokinase activator by structure-based virtual ligand screening
DOI: 10.1038/srep44681
发表时间: 2017-03-20
期刊: Scientific Reports
影响因子: 4.259
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.nature.com/articles/srep44681


5.论文标题: Effect of sarcolipin-mediated cell transdifferentiation in sarcopenia-associated skeletal muscle fibrosis
DOI: 10.1016/j.yexcr.2020.111890
发表时间: 2020-02-05
期刊: EXPERIMENTAL CELL RESEARCH
影响因子: 3.905
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0014482720300896


6.论文标题: Type 2 diabetes-induced overactivation of P300 contributes to skeletal muscle atrophy by inhibiting autophagic flux
DOI: 10.1016/j.lfs.2020.118243
发表时间: 2020-08-10
期刊: LIFE SCIENCES
影响因子: 3.647
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0024320520309954


7.论文标题: Impaired autophagic flux contributes to muscle atrophy in obesity by affecting muscle degradation and regeneration
DOI: 10.1016/j.bbrc.2020.02.110
发表时间: 2020-02-24
期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
影响因子: 2.985
货号: ZQ0092
产品名称: C2C12 myoblasts
原文链接: https://www.sciencedirect.com/science/article/pii/S0006291X20303843


8.论文标题: Effects of acute hypoxia exposure with different durations on activation of Nrf2-ARE pathway in mouse skeletal muscle
DOI: 10.1371/journal.pone.0208474
发表时间: 2018-12-04
期刊: PLoS One
影响因子: 2.766
货号: ZQ0092
产品名称: C2C12 cells
原文链接: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0208474


9.论文标题: Effect of Hypoxia on the Muscle Fiber Switching Signal Pathways CnA/NFATc1 and Myostatin in Mouse Myocytes
DOI: 10.1016/j.acthis.2019.04.001
发表时间: 2019-04-30
期刊: ACTA HISTOCHEMICA
影响因子: 2.479
货号: ZQ0092
产品名称: C2C12 myoblasts

原文链接: https://www.sciencedirect.com/science/article/pii/S0065128118304355



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C2C12 cells: biophysical, biochemical, and immunocytochemical properties.
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C2C12 murine myoblasts as a model of skeletal muscle development: morpho-functional characterization.
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Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum-free medium AIM-V.
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Evaluation of serum-free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability.
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Humphrey G.W., Mekhedov E., Blank P.S., de Morree A., Pekkurnaz G., Nagaraju K., Zimmerberg J.
GREG cells, a dysferlin-deficient myogenic mouse cell line.
Exp. Cell Res. 318:127-135(2012)


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Willkomm L., Schubert S., Jung R., Elsen M., Borde J., Gehlert S., Suhr F., Bloch W.
Lactate regulates myogenesis in C2C12 myoblasts in vitro.
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18.PubMed=25277546; DOI=10.1186/1471-2164-15-847
Didion J.P., Buus R.J., Naghashfar Z., Threadgill D.W., Morse H.C. III, Pardo-Manuel de Villena F.
SNP array profiling of mouse cell lines identifies their strains of origin and reveals cross-contamination and widespread aneuploidy.
BMC Genomics 15:847.1-847.11(2014)


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