欢迎您访问中乔新舟官网!400-038-9959我的购物车(0)
您当前的位置:首页 > 产品中心
LS 174T人结肠腺癌细胞(STR鉴定)
英文名:LS 174T
货号:ZQ0127
价格:¥1600.00
加入购物车,提交订单信息之后,我们会第一时间与您取得联系!
推荐组合

LS 174T人结肠腺癌细胞(STR鉴定)

¥1600.00
+

LS 174T [LS174T]人结肠腺癌细胞专用培养基

¥350.00 ¥480.00

配套完培,省时省力,单买细胞无优惠

=

细胞套餐惊爆价

¥1950 ¥2080.00
加入购物车
  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

LS 174T人结肠腺癌细胞

货号

ZQ0127

产品介绍

LS 174T是LS 180 (ATCC CL 187)结肠腺癌细胞株的胰蛋白酶化变种。 它比亲本更易传代,象LS 180一样生成大量的癌胚抗原(CEA)。 电镜研究表明有丰富的微丝和细胞质粘液素液泡。 直肠抗原3阳性。 p53抗原表达阴性,但mRNA表达阳性。 与ATCC CL-187来源于同一个肿瘤。LS 174T细胞角蛋白染色阳性。 癌基因c-myc, N-myc, H-ras, N-ras, Myb, 和 fos的表达呈阳性。 癌基因k-ras和sis的表达未做检测。

注意事项:
(1)LS 174T细胞消化传代后细胞24-48小时完全贴壁,培养需48小时后换液操作。细胞消化后细胞贴壁性差,可参考使用CorningCellBIND(3289)培养瓶。或者使用人纤连蛋白(中乔新舟  货号:CSP044)包被培养皿.
(2)细胞呈块状形态生长,细胞低密度生长为上皮样多边形细胞,高密度状态时有圆细胞和多边形细胞两种贴壁细胞形态。消化时若细胞
大部分变小细胞团并脱落即可停止消化。

种属

性别/年龄

女/58岁

组织

结肠

疾病

杜克斯氏B型,结肠直肠腺癌

细胞类型

 肿瘤细胞

形态学

上皮细胞

生长方式

贴壁

倍增时间

大约20~40小时 

培养基和添加剂

DMEM高糖(品牌:中乔新舟 货号:ZQ-100)+10%FBS(品牌:中乔新舟 货号:ZQ500-A)+1%双抗(中乔新舟   货号:CSP006

推荐完全培养基货号

ZM0127

生物安全等级

BSL-1

STR位点信息

Amelogenin: X

CSF1PO: 10, 13, 14

D13S317: 10

D16S539: 11, 13

D5S818: 11, 15, 16

D7S820: 10.3, 11

TH01: 6, 7

TPOX: 8, 9

vWA: 15, 17, 18

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 

基因表达

 

保藏机构

ATCC; CL-188 ATCC; CCL-188 BCRC; 60053 DSMZ; ACC-759 ECACC; 87060401

供应限制

仅供科研使用

货号

ZQ0127

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


1、论文标题: Mechanical stimulation activates Piezo1 to promote mucin2 expression in goblet cells
DOI: 10.1111/jgh.15596
发表时间: 2021-07-06
期刊: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
影响因子: 4.029
货号: ZQ0127
产品名称: LS174T cell line

原文链接: https://onlinelibrary.wiley.com/doi/abs/10.1111/jgh.15596


2、PubMed=24042735; DOI=10.1038/oncsis.2013.35

Ahmed D., Eide P.W., Eilertsen I.A., Danielsen S.A., Eknaes M., Hektoen M., Lind G.E., Lothe R.A.
Epigenetic and genetic features of 24 colon cancer cell lines.
Oncogenesis 2:e71.1-e71.8(2013)


3、PubMed=24755471; DOI=10.1158/0008-5472.CAN-14-0013
Mouradov D., Sloggett C., Jorissen R.N., Love C.G., Li S., Burgess A.W., Arango D., Strausberg R.L., Buchanan D., Wormald S., O'Connor L., Wilding J.L., Bicknell D.C., Tomlinson I.P.M., Bodmer W.F., Mariadason J.M., Sieber O.M.
Colorectal cancer cell lines are representative models of the main molecular subtypes of primary cancer.
Cancer Res. 74:3238-3247(2014)


4、PubMed=24840470; DOI=10.1016/j.jprot.2014.05.002
Chik J.H.L., Zhou J., Moh E.S.X., Christopherson R., Clarke S.J., Molloy M.P., Packer N.H.
Comprehensive glycomics comparison between colon cancer cell cultures and tumours: implications for biomarker studies.
J. Proteomics 108:146-162(2014)


5、PubMed=25485619; DOI=10.1038/nbt.3080
Klijn C., Durinck S., Stawiski E.W., Haverty P.M., Jiang Z.-S., Liu H.-B., Degenhardt J., Mayba O., Gnad F., Liu J.-F., Pau G., Reeder J., Cao Y., Mukhyala K., Selvaraj S.K., Yu M.-M., Zynda G.J., Brauer M.J., Wu T.D., Gentleman R.C., Manning G., Yauch R.L., Bourgon R., Stokoe D., Modrusan Z., Neve R.M., de Sauvage F.J., Settleman J., Seshagiri S., Zhang Z.-M.
A comprehensive transcriptional portrait of human cancer cell lines.
Nat. Biotechnol. 33:306-312(2015)


6、PubMed=25877200; DOI=10.1038/nature14397
Yu M., Selvaraj S.K., Liang-Chu M.M.Y., Aghajani S., Busse M., Yuan J., Lee G., Peale F.V., Klijn C., Bourgon R., Kaminker J.S., Neve R.M.
A resource for cell line authentication, annotation and quality control.
Nature 520:307-311(2015)


7、PubMed=25926053; DOI=10.1038/ncomms8002
Medico E., Russo M., Picco G., Cancelliere C., Valtorta E., Corti G., Buscarino M., Isella C., Lamba S., Martinoglio B., Veronese S., Siena S., Sartore-Bianchi A., Beccuti M., Mottolese M., Linnebacher M., Cordero F., Di Nicolantonio F., Bardelli A.
The molecular landscape of colorectal cancer cell lines unveils clinically actionable kinase targets.
Nat. Commun. 6:7002.1-7002.10(2015)


8、PubMed=25944804; DOI=10.1158/1078-0432.CCR-14-2457
Bazzocco S., Dopeso H., Carton-Garcia F., Macaya I., Andretta E., Chionh F., Rodrigues P., Garrido M., Alazzouzi H., Nieto R., Sanchez A., Schwartz S. Jr., Bilic J., Mariadason J.M., Arango D.
Highly expressed genes in rapidly proliferating tumor cells as new targets for colorectal cancer treatment.
Clin. Cancer Res. 21:3695-3704(2015)


9、PubMed=26589293; DOI=10.1186/s13073-015-0240-5
Scholtalbers J., Boegel S., Bukur T., Byl M., Goerges S., Sorn P., Loewer M., Sahin U., Castle J.C.
TCLP: an online cancer cell line catalogue integrating HLA type, predicted neo-epitopes, virus and gene expression.
Genome Med. 7:118.1-118.7(2015)


10、PubMed=26537799; DOI=10.1074/mcp.M115.051235
Holst S., Deuss A.J.M., van Pelt G.W., van Vliet S.J., Garcia-Vallejo J.J., Koeleman C.A.M., Deelder A.M., Mesker W.E., Tollenaar R.A.E.M., Rombouts Y., Wuhrer M.
N-glycosylation profiling of colorectal cancer cell lines reveals association of fucosylation with differentiation and caudal type homebox 1 (CDX1)/villin mRNA expression.
Mol. Cell. Proteomics 15:124-140(2016)


11、PubMed=28196595; DOI=10.1016/j.ccell.2017.01.005
Li J., Zhao W., Akbani R., Liu W.-B., Ju Z.-L., Ling S.-Y., Vellano C.P., Roebuck P., Yu Q.-H., Eterovic A.K., Byers L.A., Davies M.A., Deng W.-L., Gopal Y.N.V., Chen G., von Euw E.M., Slamon D.J., Conklin D., Heymach J.V., Gazdar A.F., Minna J.D., Myers J.N., Lu Y.-L., Mills G.B., Liang H.
Characterization of human cancer cell lines by reverse-phase protein arrays.
Cancer Cell 31:225-239(2017)


12、PubMed=28683746; DOI=10.1186/s12943-017-0691-y
Berg K.C.G., Eide P.W., Eilertsen I.A., Johannessen B., Bruun J., Danielsen S.A., Bjornslett M., Meza-Zepeda L.A., Eknaes M., Lind G.E., Myklebost O., Skotheim R.I., Sveen A., Lothe R.A.
Multi-omics of 34 colorectal cancer cell lines -- a resource for biomedical studies.
Mol. Cancer 16:116.1-116.16(2017)


13、PubMed=29101300; DOI=10.15252/msb.20177701
Frejno M., Zenezini Chiozzi R., Wilhelm M., Koch H., Zheng R.-S., Klaeger S., Ruprecht B., Meng C., Kramer K., Jarzab A., Heinzlmeir S., Johnstone E., Domingo E., Kerr D., Jesinghaus M., Slotta-Huspenina J., Weichert W., Knapp S., Feller S.M., Kuster B.
Pharmacoproteomic characterisation of human colon and rectal cancer.
Mol. Syst. Biol. 13:951-951(2017)


公司简介 / Company profile
上海中乔新舟生物科技有限公司
Shanghai Zhong Qiao Xin Zhou Biotechnology Co.,Ltd.
           上海中乔新舟生物科技有限公司(官网:www.zqxzbio.com)成立于2011年,历经...
联系我们 / Contact
电 话:021-56760357;021-56760351
传 真:
邮 箱:sales@zqxzbio.com
邮 编:200439
地 址:上海市宝山区长江南路180号
Copyright © 2014 ZQXZBIO All rights reserved.
技术支持:中乔新舟沪ICP备14008091号