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NCI-H716 [H716]人结直肠腺癌细胞(STR鉴定)
英文名:NCI-H716 [H716]
货号:ZQ0326
价格:¥1800.00
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NCI-H716 [H716]人结直肠腺癌细胞(STR鉴定)

¥1800.00
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NCI-H716 [H716]人结直肠腺癌细胞专用培养基

¥350.00 ¥450.00

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¥2150 ¥2250.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

NCI-H716 [H716]人结直肠腺癌细胞

货号

ZQ0326

产品介绍

NCI-H716细胞系是一种源自结肠的人类腺癌细胞系。它是建立在转移部位的腹水,一个33岁的白人男性。与其它结直肠癌细胞系不同,这株细胞有多巴脱羧酶,细胞质中有核心致密的内分泌型颗粒。 这株细胞不表达TAG-72 或CA19-9抗原,也不生成癌胚抗原(CEA)。

NCI-H716细胞系的一个显著特征是能够表达和分泌肠内分泌激素,特别是胰高血糖素样肽1 (glucagon-like peptide 1, GLP-1),这使得它在肠道激素生理学和肠内分泌系统的研究中具有重要意义。这方面对糖尿病研究至关重要,特别是在研究胰岛素分泌和葡萄糖稳态的激素调节的背景下。
该细胞适合以漂浮聚集体或悬浮培养的方式生长,这对于上皮来源的细胞来说有些不寻常。在悬浮中生长的能力允许在三维培养环境中研究细胞相互作用和信号通路,这比传统的单层培养更能模拟体内条件。NCI-H716细胞系已被广泛用于探索激素分泌、药物反应以及肠道上皮细胞与微生物群相互作用的信号转导途径。使用该细胞系的研究对了解胃肠道疾病的病理生理学和针对肠-脑轴的治疗策略的发展做出了重大贡献。
此外,NCI-H716细胞被用于测试治疗性化合物对分泌和受体反应的潜在影响。它们独特的激素特征也使它们能够用于与代谢紊乱和肥胖相关的药效学研究和药物发现。因此,NCI-H716在胃肠道和代谢疾病的基础研究和临床应用之间架起了桥梁,是转化医学的重要工具。

注意事项:
NCI-H716细胞悬浮聚团生长。细胞传代或换液都应该冲散细胞团块呈单细胞后进行培养。

种属

性别/年龄

男/33岁

组织

盲肠

疾病

结肠直肠腺癌

细胞类型

肿瘤细胞 

形态学

上皮细胞,悬浮并部分聚团生长

生长方式

半贴半悬

倍增时间

大约50小时 

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0326

生物安全等级

BSL-1

STR位点信息

Amelogenin: X

CSF1PO: 11

D13S317: 8,11

D16S539: 11,12

D5S818: 11

D7S820: 10,11

TH01: 6,9.3

TPOX: 8,11

vWA: 16,17

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 ***

基因表达

 ***

保藏机构

ATCC; CCL-251 BCRC; 60517 KCLB; 10251

供应限制

仅供科研使用

货号

ZQ0326

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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