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DLD-1人结直肠腺癌上皮细胞(STR鉴定)
英文名:DLD-1
货号:ZQ0325
价格:¥1600.00
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推荐组合

DLD-1人结直肠腺癌上皮细胞(STR鉴定)

¥1600.00
+

DLD-1人结直肠腺癌上皮细胞专用培养基

¥350.00 ¥450.00

配套完培,组合使用,省时省力

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细胞套餐惊爆价

¥1950 ¥2050.00
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  • 产品说明
  • 产品规格
  • 参考文献
  • STR鉴定

产品名称

DLD-1人结直肠腺癌上皮细胞

货号

ZQ0325

产品介绍

DLD-1 是1977-1979年间D.L. Dexter和同事分离的两株结直肠腺癌细胞株中的一株。 在ATCC和其它地方进行的DNA fingerprinting和染色体组型分析表明这株细胞与HCT-15 (CCL-225)相似,说明这两者是来自同一个人的不同克隆。 他们的遗传起源可通过DNA fingerprinting证实,但染色体组型分析显示它们缺乏染色体标记一致改变或数目上一致改变。 细胞的CSAp阴性(CSAp-)。 DLD-1 细胞的 p53 抗原表达呈阳性(p53 抗原产生了一个C -> T点突变导致241位的Ser -> Phe)。 角蛋白免疫过氧化物酶染色阳性。 癌基因c-myc, K-ras, H-ras, N-ras, myb, sis 和fos的表达呈阳性。 癌基因N-myc的表达未做检测。 表达肿瘤特异性核基质蛋白CC-2, CC-3, CC-4, CC-5 和 CC-6。 1979年提交到ATCC的培养物代数不明且污染了支原体。 其后经过12周多种抗生素联合培养处理。 处理之后每周用Hoechst染色和标准培养法检测。 其后连续11个月不加抗生素培养,所有的检测呈阴性。

种属

性别/年龄

男/成年人

组织

结肠

疾病

杜克斯氏C型,结肠直肠腺癌

细胞类型

肿瘤细胞

形态学

上皮的

生长方式

贴壁

倍增时间

大约20~48小时 

培养基和添加剂

RPMI-1640(中乔新舟 货号:ZQ-200)+10%胎牛血清(中乔新舟 货号:ZQ0500)+1%P/S(中乔新舟  货号:CSP006

推荐完全培养基货号

ZM0325

生物安全等级

BSL-1

STR位点信息

Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 8,11

D16S539: 12,13

D5S818: 13

D7S820: 10,12

TH01: 7,9.3

TPOX: 8,11

vWA: 18,19

培养条件

95%空气,5%二氧化碳;37℃

抗原表达/受体表达

 

基因表达

 

保藏机构

ATCC; CCL-221 BCRC; 60132 DSMZ; ACC-278 ECACC; 90102540

供应限制

仅供科研使用

货号

ZQ0325

发货规格

活细胞:T25培养瓶*1瓶或者1ml 冻存管*1支(细胞量约为5 x 10^5 cells/vial)二选一

发货形式

活细胞:常温运输;冻存管:干冰运输

储存温度

活细胞:培养箱;冻存管:液氮罐

产地

中国

供应限制

仅供科研使用


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